变温条件下铃木氏果蝇的生长发育规律
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摘要
【目的】明确变温对铃木氏果蝇(Drosophila suzukii)生长发育的影响,为铃木氏果蝇发生的预测预报及综合防治提供科学依据。【方法】以铃木氏果蝇为材料,在室内人工气候箱内设14～20、19～25、24～30、26～32和29～35℃5组变温饲养处理,测定铃木氏果蝇各发育阶段的发育历期,并用表现型(Performance)模型对铃木氏果蝇各阶段发育速率数据进行拟合分析。【结果】14～20、19～25、24～30和26～32℃变温条件下铃木氏果蝇均能完成整个世代生长发育,且各阶段的发育历期均随温度的升高而缩短,全代发育历期分别为33.81±0.64、16.64±0.34、13.16±0.37和12.26±0.69 d,29～35℃变温条件下铃木氏果蝇化蛹后蛹不能正常发育。在变温条件下,铃木氏果蝇世代的生长发育符合Performance非线性模型,该模型对其卵期、幼虫期和蛹期拟合的R2均在0.9000以上,预测得出卵、幼虫和蛹的发育起点温度分别为9.1、10.5和12.3℃,发育终止温度分别为32.2、32.5和29.5℃。【结论】在14～20、19～25、24～30和26～32℃变温条件下铃木氏果蝇均能完成整个世代生长发育,在29～35℃变温条件下不能完成世代生长发育。可运用Performance模型描述变温对铃木氏果蝇生长发育的影响。
【Objective】In order to provide the basic reference for the future population dynamics prediction and integrated control for Drosophila suzukii,this study focused on the growth and development characteristics of D. suzukii under fluctuating temperatures.【Method】D. suzukii population samples was raised in manual climatic box in the lab under five fluctuating temperature conditions,including 14-20,19-25,24-30,26-32 and 29-35 ℃. The differences of the developmental duration of each stage were detected. Then,Performance model was used to fit the growth rates of each stage of D. suzukii.【Result】The whole growth and development stages of D. suzukii could be completed under four fluctuating temperature conditions including 14-20,19-25,24-30 and 26-32 ℃,and developmental durations of each stage were shortened with the increasing temperature. The complete developmental durations of D. suzukii were 33.81±0.64,16.64±0.34,13.16 ± 0.37 and 12.26 ± 0.69 d,respectively under the above four fluctuating temperatures. However,no pupae could develop into adult under 29-35 ℃ situation. Under the fluctuating temperature conditions,the growth of D. suzukii was in line with Performance nonlinear model. The R2 of egg stage,larval stage and pupa stage fitted by this model was above 0.9000. The predicted initial temperatures for development for egg,larva and pupa were 9.1,10.5 and 12.3 ℃,and the stopping temperatures were 32.2,32.5 and 29.5 ℃.【Conclusion】Under the fluctuating temperature conditions(14-20,19-25,24-30 and 26-32 ℃),the whole growth and development stages of D. suzukii can be completed,but D.suzukii cannot develop to adult under 29-35 ℃. Performance model can describe the effects of fluctuating temperatures on development of D. suzukii.
【Objective】In order to provide the basic reference for the future population dynamics prediction and integrated control for Drosophila suzukii,this study focused on the growth and development characteristics of D. suzukii under fluctuating temperatures.【Method】D. suzukii population samples was raised in manual climatic box in the lab under five fluctuating temperature conditions,including 14-20,19-25,24-30,26-32 and 29-35 ℃. The differences of the developmental duration of each stage were detected. Then,Performance model was used to fit the growth rates of each stage of D. suzukii.【Result】The whole growth and development stages of D. suzukii could be completed under four fluctuating temperature conditions including 14-20,19-25,24-30 and 26-32 ℃,and developmental durations of each stage were shortened with the increasing temperature. The complete developmental durations of D. suzukii were 33.81±0.64,16.64±0.34,13.16 ± 0.37 and 12.26 ± 0.69 d,respectively under the above four fluctuating temperatures. However,no pupae could develop into adult under 29-35 ℃ situation. Under the fluctuating temperature conditions,the growth of D. suzukii was in line with Performance nonlinear model. The R2 of egg stage,larval stage and pupa stage fitted by this model was above 0.9000. The predicted initial temperatures for development for egg,larva and pupa were 9.1,10.5 and 12.3 ℃,and the stopping temperatures were 32.2,32.5 and 29.5 ℃.【Conclusion】Under the fluctuating temperature conditions(14-20,19-25,24-30 and 26-32 ℃),the whole growth and development stages of D. suzukii can be completed,but D.suzukii cannot develop to adult under 29-35 ℃. Performance model can describe the effects of fluctuating temperatures on development of D. suzukii.
引文
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李长春,王永,姚国新,戴余军,王立华,李国元.2018.镉在果蝇体内的积累及对其生长发育的影响[J].河南农业科学,47(8):83-87.[Li C C,Wang Y,Yao G X,Dai Y J,Wang L H,Li G Y.2018.Bioaccumulation of cadimium and its effects on the growth and development of drosophila[J].Journal of Henan Agricultural Sciences,47(8):83-87.]
李德友,陈小均,袁洁,胡吉峰.2011.樱桃果蝇生物学特性观察及其防治药剂的筛选[J].贵州农业科学,39(8):92-94.[Li D Y,Chen X J,Yuan J,Hu J F.2011.Biological character obersation and insecticide screening of Drosophila melanogaster on cherry[J].Guizhou Agricultural Science,39(8):92-94.]
廖任娅.2016.铃木氏果蝇的生态适应性研究[D].雅安:四川农业大学.[Liao R Y.2016.Ecological adaptability of Drosophila suzukii(Matsumura)(Diptera:Drosophilidae)[D].Ya’an:Sichuan Agricultural University.]
刘华,江本霞,潘冬,曾正国.2015.斑翅果蝇综合防治技术[J].湖北植保,(6):28-29.[Liu H,Jiang B X,Pan D,Zeng Z G.2015.Integrated management of Drosophila suzukii[J].Hubei Plant Protection,(6):28-29.]
刘庆忠,王晓芳,王甲威,朱东姿.2014.斑翅果蝇在甜樱桃、蓝莓等果树上的发生危害与防治策略[J].落叶果树,46(6):1-3.[Liu Q Z,Wang X F,Wang J W,Zhu D Z.2014.Occurrence and control strategies of Drosophila suzukii on sweet cherry,blueberry and other fruit trees[J].Deciduous Fruits,46(6):1-3.]
潘飞,陈绵才,肖彤斌,吉训聪,谢圣华.2014.变温对昆虫生长发育和繁殖影响的研究进展[J].环境昆虫学报,36(2):240-246.[Pan F,Chen M C,Xiao T B,Ji X C,Xie S H.2014.Research advances on effect of variable temperature on growth,development and reproduction of insect[J].Journal of Environmental Entomology,36(2):240-246.]
时培建,池本孝哉,戈峰.2011.温度与昆虫生长发育关系模型的发展与应用[J].应用昆虫学报,48(5):1149-1160.[Shi P J,Ikemoto T,Ge F.2011.Development and application of models for describing the effects of temperature on insects’growth and development[J].Journal of Applied Entomology,48(5):1149-1160.]
吴军,廖太林,孙鹏,师振华,陈集翰.2013.斑翅果蝇生物学特性研究[J].植物检疫,(5):36-42.[Wu J,Liao T L,Sun P,Shi Z H,Chen J H.2013.Bionomics of Drosophila suzukii Matsumura,1931(Diptera:Drosophilidae)[J]Plant Quarantine,(5):36-42.]
吴坤君,龚佩瑜,阮永明.2009.用非线性模型估测恒温和变温下棉铃虫蛹的发育率[J].昆虫学报,52(6):640-650.[Wu K J,Gong P Y,Ruan Y M.2009.Estimating developmental rates of Helicoverpa armigera(Lepidoptera:Noctuidae)pupae at constant and alternating temperatures by nonlinear models[J].Acta Entomologica Sinica,52(6):640-650.]
伍苏然,太红坤,李正跃,王旭,杨仕生,孙文.2007.樱桃果蝇田间诱捕方法比较[J].云南农业大学学报,22(5):776-778.[Wu S R,Tai H K,Li Z Y,Wang X,Yang S S,Sun W.2007.Field evaluation of different trapping methods of cherry fruit fly Drosophila suzukii[J].Journal of Yunnan Agricultural University,22(5):776-778.]
于毅,王静,陶云荔,国栋,褚栋.2013.铃木氏果蝇不同地理种群中Wolbachia的检测和系统发育分析[J].昆虫学报,56(3):323-328.[Yu Y,Wang J,Tao Y L,Guo D,Chu D.2013.Detection and phylogenetic analysis of Wolbachia in different geographical populations of Drosophila suzukii(Diptera:Drosophilidae)[J].Acta Entomologica Sinica,56(3):323-328.]
赵远鹏,陈晓,陈婷婷,吴道慧,肖春,张帅,张立敏.2018.变温下铃木氏果蝇年龄-阶段两性生命表研究[J].植物保护,44(3):86-91.[Zhao Y P,Chen X,Chen T T,Wu D H,Xiao C,Zhang S,Zhang L M.2018.Age-stage twosex life table of Drosophila suzukii(Diptera:Drosophilidae)at fluctuating temperatures[J].Plant Protection,44(3):86-91.]
张艺馨,郭洁,周锐,廖太林,吴晓丰,师振华.2017.斑翅果蝇卵致死低温研究[J].植物检疫,31(3):21-24.[Zhang YX,Guo J,Zhou R,Liao T L,Wu X F,Shi Z H.2017.Study on the lethal low temperature of the egg of Drosophila suzukii Matsumura[J].Plant Quarantine,31(3):21-24.]
Asplen M K,Anfora G,Biondi A,Choi D S,Chu D,Daane KM,Gibert P,Gutierrez A P,Hoelmer K A,Hutchison WD,Isaacs R,Jiang Z L,Kárpáti Z,Kimura M T,Pascual M,Philips C R,Plantamp C,Ponti L,Vétek G,Vogt H,Walton V M,Yu Y,ZappalàL,Desneux N.2015.Invasion biology of spotted wing Drosophila(Drosophilasuzukii):A global perspective and future priorities[J].Journal of Pest Science,88(3):469-494.
Calabria G,Máca J,B?chli G,Serra L,Pascual M.2012.First records of the potential pest species Drosophila suzukii(Diptera:Drosophilidae)in Europe[J].Journal of Applied Entomology,136(1-2):139-147.
Cini A,Ioriatti C,Anfora G.2012.A review of the invasion of Drosophila suzukii in Europe and a draft research agenda for integrated pest management[J].Bulletin of Insectology,65(1):149-160.
Doucet D,Walker V K,Qin W.2009.The bugs that came in from the cold:Molecular adaptations to low temperatures in insects[J].Cellular and Molecular Life Sciences,66:1404-1418.
Hauser M,GaimariS,Damus M.2009.Drosophila suzukiinew to North America[J].Fly Times,43:12-15.
Hauser M.2011.A historic account of the invasion of Drosophila suzukii(Matsumura)(Diptera:Drosophilidae)in the continental United States,with remarks on their identification[J].Pest Management Science,67(11):1352-1357.
Huey R B,Stevenson R D.1979.Integrating thermal physiology and ecology of ectotherms:A discussion of approaches[J].American Zoologist,19(1):357-366.
Kimura M T.2004.Cold and heat tolerance of drosophilid flies with reference to their latitudinal distributions[J].Oecologia,140(3):442-449.
Mironidis G K.2008.Development,survivorship,and reproduction of Helicoverpa armigera(Lepidoptera:Noctuidae)under fluctuating temperatures[J].Environmental Entomology,37(1):16-28.
Sasaki M,Sato R.1995.Bionomics of the cherry drosophila,Drosophila suzukii Matsumura(Diptera:Drosophilidae)in Fukushima Prefecture[Honshu].2.Overwintering and number of gerenations[J].Annunal Report of the Society of Plant Protection of North Japan,46:167-169.
Shi P J,Ge F.2010.A comparison of different thermal performance functions describing temperature-dependent development rates[J].Journal of Thermal Biology,35(5):225-231.
Shi P P J,Ge F,Sun Y C,Chen C L.2011.A simple model for describing the effect of temperature on insect developmental rate[J].Journal of Asia-Pacific Entomology,14(1):15-20.
Tochen S,Dalton D T,Wiman N,Hamm C,Shearer P W,Walton V M.2014.Temperature-related development and population parameters for Drosophila suzukii(Diptera:Drosophilidae)on cherry and blueberry[J].Environmental Entomology,43(2):501-510.
Walsh D B,Bolda M P,Goodhue R E,Dreves A J,Lee J,Bruck D J,Walton V M,O’Neal S D,Zalom F G.2011.Drosophila suzukii(Diptera:Drosophilidae):Invasive pest of ripening soft fruit expanding its geographic range and damage potential[J].Journal of Integrated Pest Management,2(1):1-7.
李长春,王永,姚国新,戴余军,王立华,李国元.2018.镉在果蝇体内的积累及对其生长发育的影响[J].河南农业科学,47(8):83-87.[Li C C,Wang Y,Yao G X,Dai Y J,Wang L H,Li G Y.2018.Bioaccumulation of cadimium and its effects on the growth and development of drosophila[J].Journal of Henan Agricultural Sciences,47(8):83-87.]
李德友,陈小均,袁洁,胡吉峰.2011.樱桃果蝇生物学特性观察及其防治药剂的筛选[J].贵州农业科学,39(8):92-94.[Li D Y,Chen X J,Yuan J,Hu J F.2011.Biological character obersation and insecticide screening of Drosophila melanogaster on cherry[J].Guizhou Agricultural Science,39(8):92-94.]
廖任娅.2016.铃木氏果蝇的生态适应性研究[D].雅安:四川农业大学.[Liao R Y.2016.Ecological adaptability of Drosophila suzukii(Matsumura)(Diptera:Drosophilidae)[D].Ya’an:Sichuan Agricultural University.]
刘华,江本霞,潘冬,曾正国.2015.斑翅果蝇综合防治技术[J].湖北植保,(6):28-29.[Liu H,Jiang B X,Pan D,Zeng Z G.2015.Integrated management of Drosophila suzukii[J].Hubei Plant Protection,(6):28-29.]
刘庆忠,王晓芳,王甲威,朱东姿.2014.斑翅果蝇在甜樱桃、蓝莓等果树上的发生危害与防治策略[J].落叶果树,46(6):1-3.[Liu Q Z,Wang X F,Wang J W,Zhu D Z.2014.Occurrence and control strategies of Drosophila suzukii on sweet cherry,blueberry and other fruit trees[J].Deciduous Fruits,46(6):1-3.]
潘飞,陈绵才,肖彤斌,吉训聪,谢圣华.2014.变温对昆虫生长发育和繁殖影响的研究进展[J].环境昆虫学报,36(2):240-246.[Pan F,Chen M C,Xiao T B,Ji X C,Xie S H.2014.Research advances on effect of variable temperature on growth,development and reproduction of insect[J].Journal of Environmental Entomology,36(2):240-246.]
时培建,池本孝哉,戈峰.2011.温度与昆虫生长发育关系模型的发展与应用[J].应用昆虫学报,48(5):1149-1160.[Shi P J,Ikemoto T,Ge F.2011.Development and application of models for describing the effects of temperature on insects’growth and development[J].Journal of Applied Entomology,48(5):1149-1160.]
吴军,廖太林,孙鹏,师振华,陈集翰.2013.斑翅果蝇生物学特性研究[J].植物检疫,(5):36-42.[Wu J,Liao T L,Sun P,Shi Z H,Chen J H.2013.Bionomics of Drosophila suzukii Matsumura,1931(Diptera:Drosophilidae)[J]Plant Quarantine,(5):36-42.]
吴坤君,龚佩瑜,阮永明.2009.用非线性模型估测恒温和变温下棉铃虫蛹的发育率[J].昆虫学报,52(6):640-650.[Wu K J,Gong P Y,Ruan Y M.2009.Estimating developmental rates of Helicoverpa armigera(Lepidoptera:Noctuidae)pupae at constant and alternating temperatures by nonlinear models[J].Acta Entomologica Sinica,52(6):640-650.]
伍苏然,太红坤,李正跃,王旭,杨仕生,孙文.2007.樱桃果蝇田间诱捕方法比较[J].云南农业大学学报,22(5):776-778.[Wu S R,Tai H K,Li Z Y,Wang X,Yang S S,Sun W.2007.Field evaluation of different trapping methods of cherry fruit fly Drosophila suzukii[J].Journal of Yunnan Agricultural University,22(5):776-778.]
于毅,王静,陶云荔,国栋,褚栋.2013.铃木氏果蝇不同地理种群中Wolbachia的检测和系统发育分析[J].昆虫学报,56(3):323-328.[Yu Y,Wang J,Tao Y L,Guo D,Chu D.2013.Detection and phylogenetic analysis of Wolbachia in different geographical populations of Drosophila suzukii(Diptera:Drosophilidae)[J].Acta Entomologica Sinica,56(3):323-328.]
赵远鹏,陈晓,陈婷婷,吴道慧,肖春,张帅,张立敏.2018.变温下铃木氏果蝇年龄-阶段两性生命表研究[J].植物保护,44(3):86-91.[Zhao Y P,Chen X,Chen T T,Wu D H,Xiao C,Zhang S,Zhang L M.2018.Age-stage twosex life table of Drosophila suzukii(Diptera:Drosophilidae)at fluctuating temperatures[J].Plant Protection,44(3):86-91.]
张艺馨,郭洁,周锐,廖太林,吴晓丰,师振华.2017.斑翅果蝇卵致死低温研究[J].植物检疫,31(3):21-24.[Zhang YX,Guo J,Zhou R,Liao T L,Wu X F,Shi Z H.2017.Study on the lethal low temperature of the egg of Drosophila suzukii Matsumura[J].Plant Quarantine,31(3):21-24.]
Asplen M K,Anfora G,Biondi A,Choi D S,Chu D,Daane KM,Gibert P,Gutierrez A P,Hoelmer K A,Hutchison WD,Isaacs R,Jiang Z L,Kárpáti Z,Kimura M T,Pascual M,Philips C R,Plantamp C,Ponti L,Vétek G,Vogt H,Walton V M,Yu Y,ZappalàL,Desneux N.2015.Invasion biology of spotted wing Drosophila(Drosophilasuzukii):A global perspective and future priorities[J].Journal of Pest Science,88(3):469-494.
Calabria G,Máca J,B?chli G,Serra L,Pascual M.2012.First records of the potential pest species Drosophila suzukii(Diptera:Drosophilidae)in Europe[J].Journal of Applied Entomology,136(1-2):139-147.
Cini A,Ioriatti C,Anfora G.2012.A review of the invasion of Drosophila suzukii in Europe and a draft research agenda for integrated pest management[J].Bulletin of Insectology,65(1):149-160.
Doucet D,Walker V K,Qin W.2009.The bugs that came in from the cold:Molecular adaptations to low temperatures in insects[J].Cellular and Molecular Life Sciences,66:1404-1418.
Hauser M,GaimariS,Damus M.2009.Drosophila suzukiinew to North America[J].Fly Times,43:12-15.
Hauser M.2011.A historic account of the invasion of Drosophila suzukii(Matsumura)(Diptera:Drosophilidae)in the continental United States,with remarks on their identification[J].Pest Management Science,67(11):1352-1357.
Huey R B,Stevenson R D.1979.Integrating thermal physiology and ecology of ectotherms:A discussion of approaches[J].American Zoologist,19(1):357-366.
Kimura M T.2004.Cold and heat tolerance of drosophilid flies with reference to their latitudinal distributions[J].Oecologia,140(3):442-449.
Mironidis G K.2008.Development,survivorship,and reproduction of Helicoverpa armigera(Lepidoptera:Noctuidae)under fluctuating temperatures[J].Environmental Entomology,37(1):16-28.
Sasaki M,Sato R.1995.Bionomics of the cherry drosophila,Drosophila suzukii Matsumura(Diptera:Drosophilidae)in Fukushima Prefecture[Honshu].2.Overwintering and number of gerenations[J].Annunal Report of the Society of Plant Protection of North Japan,46:167-169.
Shi P J,Ge F.2010.A comparison of different thermal performance functions describing temperature-dependent development rates[J].Journal of Thermal Biology,35(5):225-231.
Shi P P J,Ge F,Sun Y C,Chen C L.2011.A simple model for describing the effect of temperature on insect developmental rate[J].Journal of Asia-Pacific Entomology,14(1):15-20.
Tochen S,Dalton D T,Wiman N,Hamm C,Shearer P W,Walton V M.2014.Temperature-related development and population parameters for Drosophila suzukii(Diptera:Drosophilidae)on cherry and blueberry[J].Environmental Entomology,43(2):501-510.
Walsh D B,Bolda M P,Goodhue R E,Dreves A J,Lee J,Bruck D J,Walton V M,O’Neal S D,Zalom F G.2011.Drosophila suzukii(Diptera:Drosophilidae):Invasive pest of ripening soft fruit expanding its geographic range and damage potential[J].Journal of Integrated Pest Management,2(1):1-7.