葡萄霜霉病菌长期保存方法的研究
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摘要
为了解决葡萄霜霉病菌的活体保存问题,本文开展了多种保存方法的研究。采用5%二甲基亚砜低温冷冻保存、液氮冷冻保存和琼脂粉包裹冷冻保存等三种方式,调查不同低温条件和不同保存时间对孢子囊致病性的影响。结果表明:用不同保护剂处理后葡萄霜霉病菌单斑病叶保存于-80℃中30 d,5%二甲基亚砜处理的孢子囊经回接后发病面积百分率为69.32%,其致病性明显高于液氮冷冻处理和琼脂粉包裹处理;低温保存条件下,-80℃中保存的孢子囊回接后致病性明显高于-20℃的处理;三种保存方式均表现随着保存时间的延长,致病性逐渐降低。
In order to solve the problem of Plasmopara viticola preservation, pathogenicity of sporocysts under different low temperature conditions and different storage time for 5% dimethyl sulfoxide cryopreservation, liquid nitrogen cryopreservation and agar wrapped cryopreservation influences were studied. The results showed that the sporangia preserved at-80 ℃ for 30 days, and the virulent area of sporangia treated with 5% dimethyl sulfoxide cryopreservation was 69.32%, the infected was significantly higher than that of frozen in nitrogen liquid and agar refrigeration conditions; the sporangia preserved at-80 ℃ higher than those kept at-20 ℃. Three methods pathogenicity were decreased with the extension of time, the prolongation of the pathogenicity was gradually reduced.
In order to solve the problem of Plasmopara viticola preservation, pathogenicity of sporocysts under different low temperature conditions and different storage time for 5% dimethyl sulfoxide cryopreservation, liquid nitrogen cryopreservation and agar wrapped cryopreservation influences were studied. The results showed that the sporangia preserved at-80 ℃ for 30 days, and the virulent area of sporangia treated with 5% dimethyl sulfoxide cryopreservation was 69.32%, the infected was significantly higher than that of frozen in nitrogen liquid and agar refrigeration conditions; the sporangia preserved at-80 ℃ higher than those kept at-20 ℃. Three methods pathogenicity were decreased with the extension of time, the prolongation of the pathogenicity was gradually reduced.
引文
[1]周婷婷,金恭喜,岳永亮,等.葡萄霜霉病菌离体保存方法的研究[J].新疆农业科学, 2015, 52(12):2268-2272.
[2]GILLTS,DAVIDSONJA.Apreservationmethodfor Peronosporaviciaeconidia[J]. AustralasianPlantPathology,2005, 34(2):259-260.
[3]GULYA TJ,MASIREVICS, THOMASCE.Preservationof airdrieddownymildewsporangiainliquidnitrogenwithout cryoprotectants or controlled freezing[J]. Mycological Research,1993, 97(3):240-244.
[4]SATONM.Studiesonphysiologicalspecializationofdowny mildew of crucifers caused by Peronospora parasitica[J]. Journal of General Plant Pathology, 2000, 66(2):70-71.
[5] BROMFIELD K R, SCHMITT C G. Cryogenic storage of conidia of Peronospora tabacina[J]. Phytopathology, 1967, 57:1133.
[6]郝永娟,王万立,刘梗春,等.黄瓜霜霉病菌生物测定技术研究[J].华北农学报, 2006, 21(3):95-99.
[7]张艳菊,秦智伟,周秀艳,等.黄瓜霜霉病菌保存方法[J].植物病理学报, 2007, 37(4):438-441.
[8]杜兴兰,李正楠,姬惜珠,等.葡萄生轴霜霉菌孢子囊的长期保存[J].菌物学报, 2008, 27(6):908-914.
[9]王翠花,王洪久,王世祥.大白菜霜霉病菌保藏方法的初步研究[J].山东农业科学, 1989(4):38.
[10]尤敏,管福来,王萍,等.医学微生物教学用菌种保存方法的比较[J].潍坊医学院学报, 2004, 26(1):27-28.
[11]顾金刚,李世贵,姜瑞波.真菌保藏技术研究进展[J].菌物学报, 2007, 26(2):316-320.
[2]GILLTS,DAVIDSONJA.Apreservationmethodfor Peronosporaviciaeconidia[J]. AustralasianPlantPathology,2005, 34(2):259-260.
[3]GULYA TJ,MASIREVICS, THOMASCE.Preservationof airdrieddownymildewsporangiainliquidnitrogenwithout cryoprotectants or controlled freezing[J]. Mycological Research,1993, 97(3):240-244.
[4]SATONM.Studiesonphysiologicalspecializationofdowny mildew of crucifers caused by Peronospora parasitica[J]. Journal of General Plant Pathology, 2000, 66(2):70-71.
[5] BROMFIELD K R, SCHMITT C G. Cryogenic storage of conidia of Peronospora tabacina[J]. Phytopathology, 1967, 57:1133.
[6]郝永娟,王万立,刘梗春,等.黄瓜霜霉病菌生物测定技术研究[J].华北农学报, 2006, 21(3):95-99.
[7]张艳菊,秦智伟,周秀艳,等.黄瓜霜霉病菌保存方法[J].植物病理学报, 2007, 37(4):438-441.
[8]杜兴兰,李正楠,姬惜珠,等.葡萄生轴霜霉菌孢子囊的长期保存[J].菌物学报, 2008, 27(6):908-914.
[9]王翠花,王洪久,王世祥.大白菜霜霉病菌保藏方法的初步研究[J].山东农业科学, 1989(4):38.
[10]尤敏,管福来,王萍,等.医学微生物教学用菌种保存方法的比较[J].潍坊医学院学报, 2004, 26(1):27-28.
[11]顾金刚,李世贵,姜瑞波.真菌保藏技术研究进展[J].菌物学报, 2007, 26(2):316-320.