高产酒精酵母的分离筛选及耐受性的研究
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摘要
通过富集培养、TTC平板筛选及摇瓶复筛,从龙岩沉缸酒厂提供的发酵菌粉中分离筛选出3株发酵性能较强的菌株:菌株S-21、S-36、S-40。对这3株菌进行耐高温、耐乙醇、耐糖性等耐受性实验,发现这3株菌均具有优良的耐受性。
Three strains(S-21, S-36, S-40) with high fermentation performance were isolated from the fermentation powder provided by Longyan Chengang Winery through accumulated culture, TTC plate screening and shaking flask screening. Then high-temperature tolerance, ethanol tolerance, and sugar tolerance tests were performed on the three strains, and it was demonstrated that all the three strains had excellent tolerance.
Three strains(S-21, S-36, S-40) with high fermentation performance were isolated from the fermentation powder provided by Longyan Chengang Winery through accumulated culture, TTC plate screening and shaking flask screening. Then high-temperature tolerance, ethanol tolerance, and sugar tolerance tests were performed on the three strains, and it was demonstrated that all the three strains had excellent tolerance.
引文
[1]陈洪章,邱卫华.秸秆发酵燃料乙醇关键问题及其发展[J].化学进展,2007,19(7/8):1116-1120.
[2]蔡建军,王清成,王婷.生物质燃料综合应用技术研究进展[J].上海节能,2014(12):29-35.
[3]张美丽,毛勇,张志敏,等.微生物生产清洁能源的研究进展及前景[J].陕西农业科学,2014,60(1):50-52.
[4]李媛,张俊涛,谢红艳.生物能源技术发展和利用的现状研究[J].广东化工,2017,44(13):112.
[5]刘辉,施清.酿酒酵母在酒精发酵过程中耐受环境胁迫影响研究[J].酿酒科技,2015(9):54-57.
[6]张强,郭元,韩德明.酿酒酵母乙醇耐受性的研究进展[J].化工进展,2014,33(1):187-192.
[7]GE X Y,XU Y,CHEN X.Improve carbon metabolic flux in Saccharomyces cerevisiae at high temperature by overexpressed TSL1 gene[J].Journal of industrial microbiology&biotechnology,2013,40(3/4):345-352.
[8]HAMADA A,ONITSUKA N,NOZAKI N,et al.Isolation and some properties of shochu yeast from Hyuga-nada of the Miyazaki coast[J].Journal of the Brewing Society of Japan,2005,100(1):56-64.
[9]徐日益,梁磊,潘琢,等.耐酒精酵母菌株的选育及其交叉耐受性的研究[J].酿酒科技,2007(10):34-36.
[10]刘石雪,王乔平,唐丽薇,等.酿酒酵母乙醇耐受性的研究进展[J].中国生物工程杂志.2013,33(6):105-110.
[11]尤亮,丁东栋,崔志峰.乙醇耐受性酿酒酵母菌株选育的研究进展[J].工业微生物,2016,46(3):51-55.
[12]彭源德,朱作华,唐守伟,等.耐高温、高浓度酒精酵母的选育与耐受性能初步鉴定[J].中国麻业科学,2010,32(3):135-140.
[13]郭利美,马美范.饮料中酒精含量的分析方法研究[J].山东轻工业学院学报,2008,22(3):14-17.
[14]代秀芝.沸点测定仪法测定葡萄酒的酒精度[J].中外葡萄与葡萄酒,2003(2):47-48.
[15]胡嗣明,张天杭.酒精生产分析检验[M].北京:轻工业出版社,1983.
[2]蔡建军,王清成,王婷.生物质燃料综合应用技术研究进展[J].上海节能,2014(12):29-35.
[3]张美丽,毛勇,张志敏,等.微生物生产清洁能源的研究进展及前景[J].陕西农业科学,2014,60(1):50-52.
[4]李媛,张俊涛,谢红艳.生物能源技术发展和利用的现状研究[J].广东化工,2017,44(13):112.
[5]刘辉,施清.酿酒酵母在酒精发酵过程中耐受环境胁迫影响研究[J].酿酒科技,2015(9):54-57.
[6]张强,郭元,韩德明.酿酒酵母乙醇耐受性的研究进展[J].化工进展,2014,33(1):187-192.
[7]GE X Y,XU Y,CHEN X.Improve carbon metabolic flux in Saccharomyces cerevisiae at high temperature by overexpressed TSL1 gene[J].Journal of industrial microbiology&biotechnology,2013,40(3/4):345-352.
[8]HAMADA A,ONITSUKA N,NOZAKI N,et al.Isolation and some properties of shochu yeast from Hyuga-nada of the Miyazaki coast[J].Journal of the Brewing Society of Japan,2005,100(1):56-64.
[9]徐日益,梁磊,潘琢,等.耐酒精酵母菌株的选育及其交叉耐受性的研究[J].酿酒科技,2007(10):34-36.
[10]刘石雪,王乔平,唐丽薇,等.酿酒酵母乙醇耐受性的研究进展[J].中国生物工程杂志.2013,33(6):105-110.
[11]尤亮,丁东栋,崔志峰.乙醇耐受性酿酒酵母菌株选育的研究进展[J].工业微生物,2016,46(3):51-55.
[12]彭源德,朱作华,唐守伟,等.耐高温、高浓度酒精酵母的选育与耐受性能初步鉴定[J].中国麻业科学,2010,32(3):135-140.
[13]郭利美,马美范.饮料中酒精含量的分析方法研究[J].山东轻工业学院学报,2008,22(3):14-17.
[14]代秀芝.沸点测定仪法测定葡萄酒的酒精度[J].中外葡萄与葡萄酒,2003(2):47-48.
[15]胡嗣明,张天杭.酒精生产分析检验[M].北京:轻工业出版社,1983.