黄曲霉毒素饲料基体标准物质研究
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摘要
建立了含黄曲霉毒素B1、B2、G1、G2的饲料基体标准样品制备、定值和不确定度评估方法。饲料样品经加标、干燥、辐照灭菌、混匀、分装、密封制备而得。经过均匀性检验、稳定性检验、实验室联合定值以及不确定度评估等过程,最终确定饲料中黄曲霉毒素B1、B2、G1、G2的量值分别为(71. 23±1. 60)、(75. 53±1. 28)、(74. 87±1. 79)、(75. 77±2. 68)μg/kg。结果表明,饲料基体标准物质的均匀性良好,稳定性符合标准物质技术要求,同时分析并评估了该基体标准物质研制过程中引入的不确定度。该样品可用于饲料样品中黄曲霉毒素B1、B2、G1、G2的定性和定量检测。
A method for preparation,certification and uncertainty assessment of feed matrix reference material with Aflatoxin B1,Aflatoxin B2,Aflatoxin G1 and Aflatoxin G2 was established. Feed samples were prepared by adding standard,drying,irradiation sterilization,mixing,packing and sealing. With homogenization test,stability test,laboratory joint determination and uncertainty evaluation,the amounts of Aflatoxin B1,B2,G1 and G2 in the feed were determined,values were( 71. 23±1. 60),( 75. 53±1. 28),( 74. 87±1. 79),( 75. 77±2. 68) μg/kg.Uniformity of the feed matrix reference material was well,and its stability met the technical requirements of standard materials.Uncertainty caused by development process of the matrix reference material was also measured.The matrix reference material could be used for qualitative and quantitative detection of Aflatoxin B1,Aflatoxin B2,Aflatoxin G1,Aflatoxin G2 in feed samples.
A method for preparation,certification and uncertainty assessment of feed matrix reference material with Aflatoxin B1,Aflatoxin B2,Aflatoxin G1 and Aflatoxin G2 was established. Feed samples were prepared by adding standard,drying,irradiation sterilization,mixing,packing and sealing. With homogenization test,stability test,laboratory joint determination and uncertainty evaluation,the amounts of Aflatoxin B1,B2,G1 and G2 in the feed were determined,values were( 71. 23±1. 60),( 75. 53±1. 28),( 74. 87±1. 79),( 75. 77±2. 68) μg/kg.Uniformity of the feed matrix reference material was well,and its stability met the technical requirements of standard materials.Uncertainty caused by development process of the matrix reference material was also measured.The matrix reference material could be used for qualitative and quantitative detection of Aflatoxin B1,Aflatoxin B2,Aflatoxin G1,Aflatoxin G2 in feed samples.
引文
[1]LEONG Y H,LATIFF A A,AHMAD N I,et al.Exposure measurement of Aflatoxins and Aflatoxin metabolites in human body fluids. A short review[J]. Mycotoxin Res.,2012,28(2):79-87.
[2]LEONG Y H,ROSMA A,LATIFF A A,et al.Associations of serum Aflatoxin B1-lysine adduct level with socio-demographic factors and Aflatoxins intake from nuts and related nut products in Malaysia[J].Int.J.Hyg.Envir.Heal.,2012,215(3):368-372.
[3]LIU Y,CHANG C C H,MARSH G M,et al. Population attributable risk of Aflatoxin-related liver cancer:systematic review and meta-analysis[J]. Eur. J. Cancer,2012,48(14):2 125-2 136.
[4]WALIYAR F,OSIRU M,NTARE B R,et al.Post-harvest management of Aflatoxin contamination in groundnut[J].World Mycotoxin J.,2014,8(2):245-252.
[5]杨瑞琪.饲料中黄曲霉毒素对家畜的危害及防治[J].中国畜牧兽医文摘,2018,34(3):246.
[6]李静娜,伍雅婷,革丽亚,等.高效液相色谱法测定玉米粉中黄曲霉毒素B1含量的不确定度评定[J].公共卫生与预防医学,2015,26(3):22-25.
[7]于丽娜,王明清,张初署,等.黄曲霉毒素B1降解菌株的筛选及鉴定研究[J].食品研究与开发,2018,39(21):167-171.
[8]国家质量监督检验检疫总局,中国计量科学研究院.国家标准物质资源共享平台[DB/OL]. http://www.ncrm.org.cn
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[10]International Organization for Standardization.Reference materials-general and statistical principles for certification:ISO Guide 35—2006[S]. Beijing:China Metrology Press,2008.
[11]国家质量监督检验检疫总局.一级标准物质技术规范:JJF 1006—1994[S].北京:中国计量出版社,1994.
[12]全浩,韩永志.标准物质及其应用技术[M].北京:中国标准出版社,2003.
[13]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.标准样品工作导则:(3)标准样品定值的一般原则和统计方法:GB/T 15000.3—2008[S].北京:中国计量出版社,2008.
[14]中国国家标准化管理委员会.检测实验室中常用不确定度评定方法与表示:GB/T 27411—2012[S].北京:中国计量出版社,2013.
[15]中国实验室国家认可委员会.化学分析中不确定度的评估指南[M].北京:中国计量出版社,2006:31-36.
[16]中华人民共和国卫生部.食品中黄曲霉毒素B族和G族的测定:GB/T 5009.22—2016[S].北京:中国标准出版社,2016.
[17]杨琳芬,符金华,姜文娟,等.液相色谱-串联质谱结合谱库检索法同时测定饲料中4种黄曲霉毒素[J].中国饲料,2017,(14):28-30.
[18]刘飞,任安书,葛萍,等.在线固相萃取/高效液相色谱-四极杆/静电场轨道阱高分辨质谱检测饲料中的黄曲霉毒素[J].分析测试学报,2018,37(6):696-701.
[2]LEONG Y H,ROSMA A,LATIFF A A,et al.Associations of serum Aflatoxin B1-lysine adduct level with socio-demographic factors and Aflatoxins intake from nuts and related nut products in Malaysia[J].Int.J.Hyg.Envir.Heal.,2012,215(3):368-372.
[3]LIU Y,CHANG C C H,MARSH G M,et al. Population attributable risk of Aflatoxin-related liver cancer:systematic review and meta-analysis[J]. Eur. J. Cancer,2012,48(14):2 125-2 136.
[4]WALIYAR F,OSIRU M,NTARE B R,et al.Post-harvest management of Aflatoxin contamination in groundnut[J].World Mycotoxin J.,2014,8(2):245-252.
[5]杨瑞琪.饲料中黄曲霉毒素对家畜的危害及防治[J].中国畜牧兽医文摘,2018,34(3):246.
[6]李静娜,伍雅婷,革丽亚,等.高效液相色谱法测定玉米粉中黄曲霉毒素B1含量的不确定度评定[J].公共卫生与预防医学,2015,26(3):22-25.
[7]于丽娜,王明清,张初署,等.黄曲霉毒素B1降解菌株的筛选及鉴定研究[J].食品研究与开发,2018,39(21):167-171.
[8]国家质量监督检验检疫总局,中国计量科学研究院.国家标准物质资源共享平台[DB/OL]. http://www.ncrm.org.cn
[9]国家质量监督检验检疫总局.标准物质定值的通用原则及统计学原理:JJF 1343—2012[S].北京:中国质检出版社,2012.
[10]International Organization for Standardization.Reference materials-general and statistical principles for certification:ISO Guide 35—2006[S]. Beijing:China Metrology Press,2008.
[11]国家质量监督检验检疫总局.一级标准物质技术规范:JJF 1006—1994[S].北京:中国计量出版社,1994.
[12]全浩,韩永志.标准物质及其应用技术[M].北京:中国标准出版社,2003.
[13]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.标准样品工作导则:(3)标准样品定值的一般原则和统计方法:GB/T 15000.3—2008[S].北京:中国计量出版社,2008.
[14]中国国家标准化管理委员会.检测实验室中常用不确定度评定方法与表示:GB/T 27411—2012[S].北京:中国计量出版社,2013.
[15]中国实验室国家认可委员会.化学分析中不确定度的评估指南[M].北京:中国计量出版社,2006:31-36.
[16]中华人民共和国卫生部.食品中黄曲霉毒素B族和G族的测定:GB/T 5009.22—2016[S].北京:中国标准出版社,2016.
[17]杨琳芬,符金华,姜文娟,等.液相色谱-串联质谱结合谱库检索法同时测定饲料中4种黄曲霉毒素[J].中国饲料,2017,(14):28-30.
[18]刘飞,任安书,葛萍,等.在线固相萃取/高效液相色谱-四极杆/静电场轨道阱高分辨质谱检测饲料中的黄曲霉毒素[J].分析测试学报,2018,37(6):696-701.