姜及其相关产品中主要姜辣素成分的HPLC检测分析
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摘要
建立了一种快速、宽线性范围和较低检测限的高效液相色谱(HPLC)方法,分析姜及其相关产品中4种主要姜辣素——6-、8-、10-姜酚及6-姜醇含量,用于生姜、干姜、终端产品液态姜汁饮料和固态速溶姜茶,以及超临界CO2萃取得到富含姜辣素的姜油树脂和进一步分离纯化得到的高6-姜酚含量样品的检测及分析比较。结果表明:生姜中6-、8-、10-姜酚含量分别为1.461、0.325mg/g和0.568mg/g,未检测到6-姜醇;干姜中6-、8-、10-姜酚及6-姜醇的含量分别为8.587、1.612、3.504mg/g和0.662mg/g;3种固态速溶姜茶中的6-、8-、10-姜酚和6-姜醇含量范围在(0.432～0.453)、(0.108～0.122)、(0.185～0.234)mg/g和(0.094～0.109)mg/g,无显著性差异(P>0.05);姜汁饮料中只检测到少量6-姜酚,含量为0.004mg/L;姜油树脂中6-、8-、10-姜酚及6-姜醇的含量分别为212.803、50.831、88.004mg/g和11.411mg/g;在进一步分离纯化的高纯度6-姜酚样品中仅检测到6-、8-、10-姜酚,含量分别为891.329、79.145、18.717mg/g。检测结果满意,可实际用于姜及其各类产品中主要姜辣素含量的检测。
A high-performance liquid chromatographic(HPLC) method was established to fast analyze the content of four main gingerols(6-, 8-, 10-gingerol and 6-shogaol) in ginger and its related products, which has a wide linear range and low detection limit. The method was applied to detecting and analysising fresh ginger, dried ginger, ginger juice beverage, instant ginger tea, gingerol-rich ginger oleoresin obtained by supercritical CO_2 and further separation and purification of high 6-gingerol content sample.The results showed that the contents of 6-, 8-, 10-gingerol in fresh ginger were 1.461, 0.325 mg/g and 0.568 mg/g, respectively. No 6-gingerol was detected. The contents of 6-, 8-, 10-gingerol and 6-shogaol in dried ginger were 8.587, 1.612, 3.504 mg/g and 0.662 mg/g. Respectively, the contents of 6-, 8-, 10-gingerol and 6-shogaol in three solid instant ginger teas ranged from(0.432～0.453),(0.108～0.122),(0.1854～0.234)mg/g and(0.094～0.109)mg/g, no significant difference(P>0.05); only a small amount of 0.004 mg/L of6-gingerol was detected in ginger juice beverage; the contents of 6-, 8-, 10-gingerol and 6-shogaol in ginger oleoresin were 212.803, 50.831, 88.004 mg/g and 11.411 mg/g; and only 6-, 8-, 10-gingerol was detected in further purified 6-gingerol samples. The contents were 891.329, 79.145 mg/g and 18.717 mg/g respectively. The results were satisfactory and could be applied to the determination of the main content gingerols in ginger and its products.
A high-performance liquid chromatographic(HPLC) method was established to fast analyze the content of four main gingerols(6-, 8-, 10-gingerol and 6-shogaol) in ginger and its related products, which has a wide linear range and low detection limit. The method was applied to detecting and analysising fresh ginger, dried ginger, ginger juice beverage, instant ginger tea, gingerol-rich ginger oleoresin obtained by supercritical CO_2 and further separation and purification of high 6-gingerol content sample.The results showed that the contents of 6-, 8-, 10-gingerol in fresh ginger were 1.461, 0.325 mg/g and 0.568 mg/g, respectively. No 6-gingerol was detected. The contents of 6-, 8-, 10-gingerol and 6-shogaol in dried ginger were 8.587, 1.612, 3.504 mg/g and 0.662 mg/g. Respectively, the contents of 6-, 8-, 10-gingerol and 6-shogaol in three solid instant ginger teas ranged from(0.432～0.453),(0.108～0.122),(0.1854～0.234)mg/g and(0.094～0.109)mg/g, no significant difference(P>0.05); only a small amount of 0.004 mg/L of6-gingerol was detected in ginger juice beverage; the contents of 6-, 8-, 10-gingerol and 6-shogaol in ginger oleoresin were 212.803, 50.831, 88.004 mg/g and 11.411 mg/g; and only 6-, 8-, 10-gingerol was detected in further purified 6-gingerol samples. The contents were 891.329, 79.145 mg/g and 18.717 mg/g respectively. The results were satisfactory and could be applied to the determination of the main content gingerols in ginger and its products.
引文
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[8]胡巍耀,李赓,丁海燕,等.不同品牌姜酒中5种姜辣素的RP-HPLC检测方法研究[J].食品工业,2016,(9):285-287.
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[12]曾凡逵,黄雪松,李爱军.超临界二氧化碳萃取姜油树脂与溶剂浸提的比较[J].食品科学,2006,27(6):155-157.
[2]杨宇萍,杜晶,邓仙梅,等.姜及其不同炮制品中姜酚的含量测定[J].现代医药卫生,2015,31(22):3369-3370.
[3]张志浩.一种姜酒的制造方法[P].CN:101275107A,2008.
[4]郑灿龙,张月明,王良.姜茶保健饮料开发研究[J].食品工业科技,2006,(2):112-114.
[5]刘绍军,周丽艳,赵希艳,等.生姜大枣功能饮料的工艺研究[J].食品科学,2011,(s1):120-122.
[6]马龙利,李岗,叶菲菲,等.6-姜酚的分离纯化及抗氧化能力研究[J].食品科技,2016,(8):206-209.
[7]王丽,徐美霞,王梅,等.HPLC法测定母姜与子姜中的6-姜酚,8-姜酚和10-姜酚[J].山东科学,2013,26(1):12-15.
[8]胡巍耀,李赓,丁海燕,等.不同品牌姜酒中5种姜辣素的RP-HPLC检测方法研究[J].食品工业,2016,(9):285-287.
[9]张永鑫,李俊松,陈丽华,等.高效液相色谱法同时测定姜及其不同炮制品中5种姜辣素的含量[J].中国药学杂志,2012,47(6):471-474.
[10]Bhattarai S,Tran V H,Duke C C.Stability of[6]-gingerol and[6]-shogaol in simulated gastric and intestinal fluids[J].J Pharm Biomed Anal,2007,45(4):648-653.
[11]陈艳,周冬翠,张梅.3种方法提取生姜有效部位群并测定6-姜辣素含量[J].中国药师,2014,17(7):1099-1102.
[12]曾凡逵,黄雪松,李爱军.超临界二氧化碳萃取姜油树脂与溶剂浸提的比较[J].食品科学,2006,27(6):155-157.