PEG6000模拟干旱胁迫对2种沙冬青种子萌发的影响
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摘要
以蒙古沙冬青和新疆沙冬青为材料,在不同浓度的PEG6000(0%、4%、8%、12%、16%、20%和24%)胁迫处理下,研究干旱胁迫对种子萌发和幼苗生长的影响,探讨经PEG6000处理后复水种子的萌发恢复能力。结果表明,随着PEG6000浓度的增加,蒙古沙冬青和新疆沙冬青的种子萌发率、发芽势、日相对发芽率受到不同程度的抑制,萌发进程推迟,胚芽长和胚根长也受到抑制,萌发种子含水量下降。总体来看,PEG6000浓度越大,各指标受抑制程度越明显,另外,在同等条件的干旱胁迫下,蒙古沙冬青受抑制程度小于新疆沙冬青。经PEG6000浸种处理的种子复水后仍具有较高的恢复萌发率,新疆沙冬青在24%PEG6000处理下无种子萌发,复水后萌发率较高。由此得出,蒙古沙冬青的抗旱性高于新疆沙冬青。
In this study,Ammopiptanthus mongolicus and A.nanus were taken as the test materials to study the effects of drought stress on seed germination and seedling growth under different concentrations of PEG6000(0%,4%,8%,12%,16%,20%and 24%).The germination recovery abilities of the two species through the water addition after PEG6000 treatment was discussed.The results showed that with the increase of PEG6000 concentration,the germination,germination potential,relative daily germination rate of the seeds were inhibited with different degrees,germination process delayed,the radical and germ growth was also inhibited,and seed moisture content reduced.Overall,the effect of each index inhibition became more obvious with the increase of PEG6000 concentration.In addition,under the same conditions of drought stress,the inhibition degree of A.mongolicus was less than A.nanus.Through the water addition after PEG6000 treatment,the germination rate of seeds was still higher,and the germination recovery of A.mongolicus was higher than A.nanus,the seed germination of A.nanus under 24% PEG6000 treatment was zero,and was higher through the water addition.Thus,the drought resistance of A.mongolicus was higher than A.nanus.
In this study,Ammopiptanthus mongolicus and A.nanus were taken as the test materials to study the effects of drought stress on seed germination and seedling growth under different concentrations of PEG6000(0%,4%,8%,12%,16%,20%and 24%).The germination recovery abilities of the two species through the water addition after PEG6000 treatment was discussed.The results showed that with the increase of PEG6000 concentration,the germination,germination potential,relative daily germination rate of the seeds were inhibited with different degrees,germination process delayed,the radical and germ growth was also inhibited,and seed moisture content reduced.Overall,the effect of each index inhibition became more obvious with the increase of PEG6000 concentration.In addition,under the same conditions of drought stress,the inhibition degree of A.mongolicus was less than A.nanus.Through the water addition after PEG6000 treatment,the germination rate of seeds was still higher,and the germination recovery of A.mongolicus was higher than A.nanus,the seed germination of A.nanus under 24% PEG6000 treatment was zero,and was higher through the water addition.Thus,the drought resistance of A.mongolicus was higher than A.nanus.
引文
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[20]张云华,王荣富,阮龙.水分胁迫对甘薯叶绿素荧光和光合特性的研究[J].中国农学通报,2005,21(8):208-210.ZHANG Y H,WANG R F,RUAN L.Effects of water stress on chlorophyⅡfluorescence and photosynthesis characteristics in sweet potato[J].Chinese Agricultural Science Bulletin,2005,21(8):208-210.(in Chinese)
[21]于军,焦培培.聚乙二醇(PEG6000)模拟干旱胁迫抑制矮沙冬青种子的萌发[J].基因组学与应用生物学,2010,29(2):355-360.YU J,JIAO P P.Inhibition of seed germination of Ammmopiptanthus nanus(M.Pop.)Cheng f.under simulated drought stress with polyethylene glycol(PEG6000)[J].Genomics and Applied Biology,2010,29(2):355-360.(in Chinese)
[22]曾彦军,王彦荣,萨仁,等.几种旱生灌木种子萌发对干旱胁迫的响应[J].应用生态学报,2002,13(8):953-956.ZENG Y J,WANG Y R,SA R,et al.Response of seed germination of three xeromorphic shrubs to drought stress[J].Chinese Journal of Applied Ecology,2002,13(8):953-956.(in Chinese)
[23]张勇,薛林贵,高天鹏,等.荒漠植物种子萌发研究进展[J].中国沙漠,2005,25(1):106-112.ZHANG Y,XUE L G,GAO T P,et al.Research advance on seed germination of desert plants[J].Journal of Desert Research,2005,25(1):106-112.(in Chinese)
[24]李新荣,张新时.鄂尔多斯高原荒漠化草原与草原化荒漠灌木类群生物多样性的研究[J],应用生态学报,1999,10(6):665-669.LI X R,ZHANG X S.Biodiverstty of shrub commnity in dtsert steppe and steppe desert on Erdos Plateau[J].Chinese Journal of applied ecology,1999,10(6):665-669.(in Chinese)
[25]杨景宁,王彦荣.PEG模拟干旱胁迫对四种荒漠植物种子萌发的影响[J].草业学报,2012,21(6):23-29.YANG J N,WANG Y R.Effects of drought stress simulated by PEG on seed germination of four desert plant species[J].Acta Prataculturae Sinica,2012,21(6):23-29.(in Chinese)
[26]薛盼盼,张强,魏学智.模拟干旱胁迫对酸枣种子萌发及活力的影响[J].西北植物学报,2016,25(12):1837-1843.XUE P P,ZHANG Q,WEI X Z.Effects of simulated drought stress on germination and vigor of Zizyphus jujubaseeds[J].Acta Agriculturae Boreali-Occidentalis Sinica,2016,25(12):1837-1843.(in Chinese)
[27]李志萍,张文辉,崔豫川.PEG模拟干旱胁迫对栓皮栎种子萌发及生长生理的影响[J].西北植物学报,2013,33(10):2043-2049.LI Z P,ZHANG W H,CUI Y C.Effects of PEG simulated drought stress on seed germination and growth physiology of Quercus variabilis[J].Acta Botanica Boreali-Occidentalia Sinica,2013,33(10):2043-2049.(in Chinese)
[28]史锋厚,朱灿灿,沈永宝,等.PEG6000渗透处理对油松种子发芽的影响[J].浙江林学院学报,2008,25(3):289-292.SHI F H,ZHU C C,SHEN Y B,et al.Germination of Pinus tabulaeformis seeds with PEG 6000osmotic treatments[J].Journal of Zhejiang Forestry College,2008,25(3):289-292.(in Chinese)
[29]秦文静,梁宗锁.四种豆科牧草萌发期对干旱胁迫的响应及抗旱性评价[J].草业学报,2010,19(4):61-70.
[30]罗光宏,王进,颜霞,等.干旱胁迫对唐古特白刺种子吸胀萌发和幼苗生长的影响[J].中国沙漠,2014,34(6):1537-1543.LUO G H,WANG J,YAN X,et al.Effect of drought stress on imbibition,germination and seeding growth of Nitraria tangutorum[J].Journal of Desert Research,2014,34(6):1537-1543.(in Chinese)
[2]张刚,魏典典,邬佳宝,等.干旱胁迫下不同种源文冠果幼苗的生理反应及其抗旱性分析[J].西北林学院学报,2014,29(1):1-7.ZHANG G,WEI D D,WU J B,et al.Effects of drought stress on physiological characteristics of Xanthoceras sorbifolia from different provenances and analysis on drought-resistance in seedling stage[J].Journal of Northwest Forestry University,2014,29(1):1-7.(in Chinese)
[3] JAKAB G,TON J,FLORS V.Enhancing arabidopsis salt and drought stress tolerance by chemical priming for its abscisic acid responses[J].Plant Physiol,2005,139:267-274.
[4]金美芳,朱晓清.NaCl胁迫对油菜种子萌发和幼苗生长的影响[J].种子,2009,28(9):76-79.JIN M F,ZHU X Q.Effects of NaCl stress on seed germination and seedling growth of brassica rapa[J].Seed,2009,28(9):76-79.(in Chinese)
[5]薛建国,韩建国,王显国,等.NaCl和PEG对华北驼绒藜和梭梭种子萌发的影响[J].草地学报,2008,16(5):470-474.XUE J G,HAN J G,WANG X G,et al.Effects of NaCl and PEG on seed germination of two xerophytic species Ceratoides arborescens and Haloxylon ammodendron[J].Acta Agrestia Sinica,2008,16(5):470-474.(in Chinese)
[6]李培英,孙宗玖,阿不来提.PEG模拟干旱胁迫29份偃麦草种质种子萌发期抗旱性评价[J].中国草地学报,2010,32(1):32-39.LI P Y,SUN Z J,ABULAITI.Evaluation of drought resistance of 29accessions of Elytrigria repens at seed germination stage under PEG-6000stress[J].Chinese Journal of Grassland,2010,32(1):32-39.(in Chinese)
[7]廖友媛.PEG对翅荚决明种子萌发和幼苗生长的影响[J].湖南工业大学学报,2009,23(2):24-27.
[8]安永平,强爱玲,张嫒嫒,等.渗透胁迫下水稻种子萌发特性及抗旱性鉴定指标研究[J].植物遗传资源学报,2006,7(4):421-426.AN Y P,QIANG A L,ZHANG Y Y,et al.Study on characteristics of germination and drought-resistance index by osmotic stress in rice[J].Journal of Plant Genetic Resources,2006,7(4):421-426.(in Chinese)
[9]景蕊莲,昌小平.用渗透胁迫鉴定小麦种子萌发期抗旱性的方法分析[J].植物遗传资源学报,2003,4(4):292-296.
[10]李昌龙,尉秋实,李爱德.孑遗植物沙冬青的研究进展与展望[J].中国野生植物资源,2004,23(5):21-24.LI C L,YU Q S,LI A D.Study and application of the endangered plant Ammopiptanthus[J].Chinese Wild Plant Resources,2004,23(5):21-24.(in Chinese)
[11]刘美芹,卢存福,尹伟伦.珍稀濒危植物沙冬青生物学特性及抗逆性研究进展[J].应用与环境生物学报,2004,10(3):384-388.LIU M Q,LU C F,YIN W L.Research progress of biological characteristics and abiotics stress tolerant mechanisms of rare&endangered plant Ammopiptanthus[J].Chinese Journal of Applied and Environmental Biology,2004,10(3):384-388.(in Chinese)
[12]王庆锁,李勇,张灵芝.珍稀濒危植物沙冬青研究概况[J].生物多样性,1995,3(3):153-156.
[13]潘伯荣,尹林克.我国干旱荒漠区珍稀濒危植物资源的综合评价及合理利用[J].干旱区研究,1991,8(3):29-33.
[14]丁琼.共生菌在濒危植物沙冬青引种栽培中的应用研究[D].北京:北京林业大学,2004.
[15]王华.沙冬青与根瘤菌和丛枝菌根真菌(AMF)共生关系影响因素的研究[D].北京:北京林业大学,2008.
[16]王烨,尹林克.两种沙冬青耐盐性测定[J].干旱区研究,1991,8(2):20-22.
[17]许国英,潘伯荣,谢明玲.沙冬青生物碱成分研究[J].干旱区研究,1994,11(1):52-56.
[18]杨阳,刘秉儒,贾倩民,等.赤霉素对干旱胁迫下沙冬青种子萌发的影响[J].江苏农业科学,2014,42(5):271-274.
[19]李进军,薛斌瑞,柴宗政.民勤县荒漠-绿洲交错带梭梭种群的数量动态及分布格局[J].东北林业大学学报,2013,41(8):27-31.LI J J,XUE B R,CHAI Z Z.Quantity dynamics and distribution pattern for Haloxylon ammodendron population of the desert-oasis ecotone in Minqin county of Gansu Province,China[J].Journal of Northeast Forestry University,2013,41(8):27-31.(in Chinese)
[20]张云华,王荣富,阮龙.水分胁迫对甘薯叶绿素荧光和光合特性的研究[J].中国农学通报,2005,21(8):208-210.ZHANG Y H,WANG R F,RUAN L.Effects of water stress on chlorophyⅡfluorescence and photosynthesis characteristics in sweet potato[J].Chinese Agricultural Science Bulletin,2005,21(8):208-210.(in Chinese)
[21]于军,焦培培.聚乙二醇(PEG6000)模拟干旱胁迫抑制矮沙冬青种子的萌发[J].基因组学与应用生物学,2010,29(2):355-360.YU J,JIAO P P.Inhibition of seed germination of Ammmopiptanthus nanus(M.Pop.)Cheng f.under simulated drought stress with polyethylene glycol(PEG6000)[J].Genomics and Applied Biology,2010,29(2):355-360.(in Chinese)
[22]曾彦军,王彦荣,萨仁,等.几种旱生灌木种子萌发对干旱胁迫的响应[J].应用生态学报,2002,13(8):953-956.ZENG Y J,WANG Y R,SA R,et al.Response of seed germination of three xeromorphic shrubs to drought stress[J].Chinese Journal of Applied Ecology,2002,13(8):953-956.(in Chinese)
[23]张勇,薛林贵,高天鹏,等.荒漠植物种子萌发研究进展[J].中国沙漠,2005,25(1):106-112.ZHANG Y,XUE L G,GAO T P,et al.Research advance on seed germination of desert plants[J].Journal of Desert Research,2005,25(1):106-112.(in Chinese)
[24]李新荣,张新时.鄂尔多斯高原荒漠化草原与草原化荒漠灌木类群生物多样性的研究[J],应用生态学报,1999,10(6):665-669.LI X R,ZHANG X S.Biodiverstty of shrub commnity in dtsert steppe and steppe desert on Erdos Plateau[J].Chinese Journal of applied ecology,1999,10(6):665-669.(in Chinese)
[25]杨景宁,王彦荣.PEG模拟干旱胁迫对四种荒漠植物种子萌发的影响[J].草业学报,2012,21(6):23-29.YANG J N,WANG Y R.Effects of drought stress simulated by PEG on seed germination of four desert plant species[J].Acta Prataculturae Sinica,2012,21(6):23-29.(in Chinese)
[26]薛盼盼,张强,魏学智.模拟干旱胁迫对酸枣种子萌发及活力的影响[J].西北植物学报,2016,25(12):1837-1843.XUE P P,ZHANG Q,WEI X Z.Effects of simulated drought stress on germination and vigor of Zizyphus jujubaseeds[J].Acta Agriculturae Boreali-Occidentalis Sinica,2016,25(12):1837-1843.(in Chinese)
[27]李志萍,张文辉,崔豫川.PEG模拟干旱胁迫对栓皮栎种子萌发及生长生理的影响[J].西北植物学报,2013,33(10):2043-2049.LI Z P,ZHANG W H,CUI Y C.Effects of PEG simulated drought stress on seed germination and growth physiology of Quercus variabilis[J].Acta Botanica Boreali-Occidentalia Sinica,2013,33(10):2043-2049.(in Chinese)
[28]史锋厚,朱灿灿,沈永宝,等.PEG6000渗透处理对油松种子发芽的影响[J].浙江林学院学报,2008,25(3):289-292.SHI F H,ZHU C C,SHEN Y B,et al.Germination of Pinus tabulaeformis seeds with PEG 6000osmotic treatments[J].Journal of Zhejiang Forestry College,2008,25(3):289-292.(in Chinese)
[29]秦文静,梁宗锁.四种豆科牧草萌发期对干旱胁迫的响应及抗旱性评价[J].草业学报,2010,19(4):61-70.
[30]罗光宏,王进,颜霞,等.干旱胁迫对唐古特白刺种子吸胀萌发和幼苗生长的影响[J].中国沙漠,2014,34(6):1537-1543.LUO G H,WANG J,YAN X,et al.Effect of drought stress on imbibition,germination and seeding growth of Nitraria tangutorum[J].Journal of Desert Research,2014,34(6):1537-1543.(in Chinese)