离子液体[C_8mim]Cl对泥鳅肝脏的氧化损伤效应
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摘要
以泥鳅为实验材料,研究离子液体氯化1-辛基-3-甲基咪唑([C8mim]Cl)对其肝脏的氧化损伤效应.结果显示:各浓度组中活性氧(ROS)含量在染毒1d、4d和8d后与对照组相比显著升高,1d时含量达到峰值;脂质过氧化物(LPO)含量在染毒1d时其低、中、高浓度组与对照组相比显著升高,随着暴露时间的延长,在染毒4d和8d时各浓度组LPO含量下降并低于对照组水平;谷丙转氨酶(GPT)含量与对照组相比在低、中、高不同浓度组染毒1d、4d和8d后,均显著下降;谷胱甘肽还原酶(GR)活力在染毒1d、4d和8d后各浓度组与对照组相比均呈现出显著下降趋势,在第8d时,达到最低水平.结果表明离子液体[C8mim]Cl对泥鳅肝脏有明显的氧化损伤效应.本研究结果对于人们正确认识"绿色溶剂"离子液体的安全性及其安全使用具有重要参考价值.
In this paper,we studied oxidative damage effect of the ionic liquid 1-octyl-3-methyl imidazole chloride([C8mim]Cl)on the loach livers.Results showed that compared with control group:Reactive oxygen species(ROS)content significantly increased,and the highest level was at 1d.Compared with control group,content of lipid peroxide(LPO)at 1d significantly increased.With increasing exposures time,the content of LPO level decreased and was lower than the control group in 4d or 8d.Compared with control group,the content of GPT,were significantly decreased at 1d,4d and 8d after exposed to Ionic Liquids.Glutathione reductase(GR)activity showed a significant declining trend,and the lowest level was at 8 d.These results showed that the ionic liquid[C8mim]Cl had obvious oxidative damage effects on the liver of loach.The results of this paper have important reference for people to proply understand the safety and correct use of the "green solvent" ionic liquid.
In this paper,we studied oxidative damage effect of the ionic liquid 1-octyl-3-methyl imidazole chloride([C8mim]Cl)on the loach livers.Results showed that compared with control group:Reactive oxygen species(ROS)content significantly increased,and the highest level was at 1d.Compared with control group,content of lipid peroxide(LPO)at 1d significantly increased.With increasing exposures time,the content of LPO level decreased and was lower than the control group in 4d or 8d.Compared with control group,the content of GPT,were significantly decreased at 1d,4d and 8d after exposed to Ionic Liquids.Glutathione reductase(GR)activity showed a significant declining trend,and the lowest level was at 8 d.These results showed that the ionic liquid[C8mim]Cl had obvious oxidative damage effects on the liver of loach.The results of this paper have important reference for people to proply understand the safety and correct use of the "green solvent" ionic liquid.
引文
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[19]李效宇,曾师虎,张伟红,等.离子液体溴化1-辛基-3-甲基咪唑对太行隆肛蛙蝌蚪的毒性及其抗氧化系统的影响[J].河南师范大学学报(自然科学版),2010,38(1):138-141.
[2]Jungnickel C,uczak J,Ranke J,et al.Micelle formation of imidazolium ionic liquids in aqueous solution[J].Colloids&Surfaces A Physicochemical&Engineering Aspects,2012,316(1/3):278-284.
[3]赵卫星,姜红波,王艳,等.绿色溶剂---离子液体及其毒性[J].山东化工,2010,39(8):21-24.
[4]吴波,张玉梅,王华平.离子液体的安全性研究进展[J].化工进展,2008,27(6):814-818.
[5]石长应,王凡,张合彩,等.不同烃链长度咪唑类离子液体对日本三角涡虫的急性毒性[J].河南师范大学学报(自然科学版),2014(4):178-182.
[6]井长勤,刘振华,王燕,等.氯化1-辛基-3-甲基咪唑对EMT6细胞的毒性及其DNA损伤作用[J].河南师范大学学报(自然科学版),2014(2):124-130.
[7]Salazar-Lugo R,Mata C,Oliveros A,et al.Histopathological changes in gill,liver and kidney of neotropical fish Colossoma macropomum exposed to paraquat at different temperatures[J].Environmental Toxicology&Pharmacology,2011,31(3):490-495.
[8]Min S K,Kim J H,Kim S,et al.Various biomarker and bioindicator responses in muddy loach(Misgurnus anguillicadatus)exposed to endosulfan for 21days[J].Toxicology and Environmental Health Sciences,2010,2(2):125-131.
[9]李兴太,张春英,仲伟利,等.活性氧的生成与健康和疾病关系研究进展[J].食品科学,2016,37(13):257-270.
[10]Chen M,Yin J,Liang Y,et al.Oxidative stress and immunotoxicity induced by graphene oxide in zebrafish[J].Aquatic Toxicology,2016,174:54-60.
[11]姜晓东,吴旭干,张金彪,等.三种饵料模式对中华绒螯蟹种早期养殖性能、非特异免疫性能及抗病力的影响[J].动物学杂志,2017(1):85-96.
[12]Jiang J,Qin C,Shu X,et al.Effects of copper on induction of thiol-compounds and antioxidant enzymes by the fruiting body of Oudemansiella radicata.[J].Ecotoxicology&Environmental Safety,2015,111(2):60-65.
[13]Lin L,Zeng X L,Zhang J.Effect of profenofos poisoning on liver lipid peroxidation and liver function in rabbits[J].Chinese Journal of Clinical Rehabilitation,2004,8(21):4380-4381.
[14]Jia R,Cao L P,Du J L,et al.Effects of carbon tetrachloride on oxidative stress,inflammatory response and hepatocyte apoptosis in common carp(Cyprinus carpio)[J].Aquatic Toxicology,2014,152(152C):11-19.
[15]Xia X H,Xia X P,Huo W R,et al.Toxic effects of imidacloprid on adult loach(Misgurnus anguillicaudatus)[J].Environmental Toxicology&Pharmacology,2016,45:132-139.
[16]Bonnail E,Buruaem L M,Araujo G S,et al.Multiple Biomarker Responses in Corbicula fluminea Exposed to Copper in Laboratory Toxicity Tests[J].Archives of Environmental Contamination&Toxicology,2016,71(2):1-8.
[17]刘晓麒,曹恩华.脂质过氧化引起的DNA损伤研究进展[J].生物化学与生物物理进展,1994,21(3):218-222.
[18]佘永新,格桑顿珠,杨小梅,等.氟中毒鸡血液中SOD和LPO含量的变化[J].西南农业学报,2002,15(2):114-116.
[19]李效宇,曾师虎,张伟红,等.离子液体溴化1-辛基-3-甲基咪唑对太行隆肛蛙蝌蚪的毒性及其抗氧化系统的影响[J].河南师范大学学报(自然科学版),2010,38(1):138-141.