山萸肉饮片标准汤剂研究
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摘要
目的制备山萸肉饮片标准汤剂并进行质量研究,为相关研究与药物开发提供参考。方法根据中药饮片标准汤剂制备原则,制备15批不同产地的山萸肉饮片标准汤剂,以莫诺苷、马钱苷和山茱萸新苷作为定量检测指标,计算转移率与出膏率,并建立超高液相色谱(UPLC)特征图谱分析方法。结果 15批山萸肉饮片标准汤剂的出膏率为43.9%～48.2%,莫诺苷转移率为55.8%～90.8%,马钱苷转移率为52.8%～68.3%,山茱萸新苷转移率为47.2%～61.6%。采用《中药色谱指纹图谱相似度评价系统(2012版)》软件进行特征图谱分析,标定了13个共有峰,对15批山萸肉饮片及标准汤剂进行相似度评价,其相似度均大于0.9。结论本研究山萸肉饮片标准汤剂制备规范,测定方法准确可靠,能够反映山萸肉水煎制剂的内在质量,可为山萸肉配方颗粒的质量控制提供参考。
Objective To prepare Corni Fructus standard decoction and conduct research on quality; To provide references for research and development of related medicine. Methods Fifteen batches of Corni Fructus standard decoction were prepared according to the standardization method of TCM decoction pieces. With morroniside, loganin and cornuside as the detection indices, the transfer rate and extraction rate were calculated, and the UPLC characteristic chromatograms analysis method was established. Results According to the measurement of 15 batches of Corni Fructus standard decoctions, the extractum rate was at the range of 43.9%–48.2%, and the transfer rates of morroniside, loganin and cornuside were 55.8%–90.8%, 52.8%–68.3% and 47.2%–61.6%, respectively. Besides, Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012 edition) was used to analyze and compare the characteristic chromatograms, and 13 common peaks were determined. The similarity was over 0.9. Conclusion The preparation of Corni Fructus standard decoction is standard. The determination method is reliable and accurate, which can be used to reflect the quality of Corni Fructus standard decoctions and provide references for the quality control of Corni Fructus formula granules.
Objective To prepare Corni Fructus standard decoction and conduct research on quality; To provide references for research and development of related medicine. Methods Fifteen batches of Corni Fructus standard decoction were prepared according to the standardization method of TCM decoction pieces. With morroniside, loganin and cornuside as the detection indices, the transfer rate and extraction rate were calculated, and the UPLC characteristic chromatograms analysis method was established. Results According to the measurement of 15 batches of Corni Fructus standard decoctions, the extractum rate was at the range of 43.9%–48.2%, and the transfer rates of morroniside, loganin and cornuside were 55.8%–90.8%, 52.8%–68.3% and 47.2%–61.6%, respectively. Besides, Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012 edition) was used to analyze and compare the characteristic chromatograms, and 13 common peaks were determined. The similarity was over 0.9. Conclusion The preparation of Corni Fructus standard decoction is standard. The determination method is reliable and accurate, which can be used to reflect the quality of Corni Fructus standard decoctions and provide references for the quality control of Corni Fructus formula granules.
引文
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[2]叶贤胜,赫军,张佳琳,等.山茱萸的化学成分研究[J].中国中药杂志,2016,41(24):4605-4609.
[3]梁晋如.山茱萸的化学成分及其生物活性研究[D].西安:西北大学,2014.
[4]杨明明,袁晓旭,赵桂琴,等.山茱萸化学成分和药理作用的研究进展[J].承德医学院学报,2016,33(5):398-400.
[5]李雅梅,李华,李春荣.山茱萸化学成分及其药理作用研究进展[J].武警医学院学报,2010,19(6):500-502.
[6]袁菊丽,姜红波.山茱萸的主要化学成分及药理作用[J].化学与生物工程,2011,28(5):7-9.
[7]张兰桐,任雷鸣,温进坤.山茱萸提取液抗心律失常有效部位的研究[J].中草药,2001,32(11):47-50.
[8]谢靖屹.中药配方颗粒特点分析[J].广州医药,2011,42(2):40-42.
[9]连红兵.中药配方颗粒在临床上的利弊分析[J].内蒙古中医药,2016,35(2):101-102.
[10]孟凡辰,郭兆安.中药配方颗粒与传统中药汤剂的对比[J].中国医药科学,2015,5(3):89-90.
[11]杨晓日,孙巍.中药配方颗粒探讨[J].中国药业,2007,16(14):60.
[12]陈士林,刘安,李琦,等.中药饮片标准汤剂研究策略[J].中国中药杂志,2016,41(8):1367-1375.