基于COI基因分析的双齿蚋分子分类研究
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摘要
为了确定COI基因序列分析在蚋科中的分类地位,本研究测定了双齿蚋(Simulium bidentatum) COI基因序列,分析该基因序列特征;下载与其同源性较高的12个蚋种的COI基因序列15条,进行同源性分析和分子系统发育研究。结果显示,双齿蚋COI基因序列(DQ534946)与S. notiale、S. snowi、S. nyssa等蚋种的COI基因序列同源性最高,在贝叶斯分析所构建的分子系统进化树中,不同蚋组的序列各自聚成一枝,双齿蚋序列(DQ534946)未与其他任何蚋种聚为一枝。研究结果表明,双齿蚋COI基因序列与蚋亚属序列同源性较高,COI基因序列分析能较好建立蚋亚属内组间关系,双齿蚋未与其他蚋组序列聚为一枝,分类至淡足蚋组并不合适,支持分类至阿根蚋组。本研究进一步明确了COI基因序列分析在蚋科分子系统发育研究中的意义,并在一定程度上解决了双齿蚋在蚋科的分类争议。
In order to determine its taxonomic status of Simulium bidentatum in the family by the analysis of COI gene sequence of Simulium bidentatum, the homology and molecular phylogeny for one COI gene sequence of S.bidentatum determined and 15 COI gene sequences of 12 species downloaded from the GenBank were analyzed in this research. Research results showed that COI gene sequence of S. bidentatum and S. notiale, S. snowi, S. nyssa have the highest homology. In the phylogenetic tree based on COI gene, the sequences of the different Simulium groups formed a clade respectively, and the sequence of S. bidentatum(DQ534946) didn't form a clade with any other groups sequences. In conclusion, these results suggested that COI gene sequence of S. bidentatum and Simulium subgenus sequences have higher homology and COI gene could better discriminate Simulium subgenus at the group level. It was more accurate to class S. bidentatum into argentipes group instead of malyschevi group because S. bidentatum wasn't formed a robust clade with malyschevi group. This study further clarified the significance of COI gene sequence in the molecular Phylogeny of Simuliidae, and solved the classification status of S. bidentatum in a certain extent.
In order to determine its taxonomic status of Simulium bidentatum in the family by the analysis of COI gene sequence of Simulium bidentatum, the homology and molecular phylogeny for one COI gene sequence of S.bidentatum determined and 15 COI gene sequences of 12 species downloaded from the GenBank were analyzed in this research. Research results showed that COI gene sequence of S. bidentatum and S. notiale, S. snowi, S. nyssa have the highest homology. In the phylogenetic tree based on COI gene, the sequences of the different Simulium groups formed a clade respectively, and the sequence of S. bidentatum(DQ534946) didn't form a clade with any other groups sequences. In conclusion, these results suggested that COI gene sequence of S. bidentatum and Simulium subgenus sequences have higher homology and COI gene could better discriminate Simulium subgenus at the group level. It was more accurate to class S. bidentatum into argentipes group instead of malyschevi group because S. bidentatum wasn't formed a robust clade with malyschevi group. This study further clarified the significance of COI gene sequence in the molecular Phylogeny of Simuliidae, and solved the classification status of S. bidentatum in a certain extent.
引文
Chen H.,and Chen H.B.,2003,Sequences analysis of cytochrome C oxidase subunitⅠgene in Aedes albopictus from different geographic strains in China,Zhongguo Liuxingbingxue Zazhi(Chinese Journal of Epidemiology),24(6):491-493(陈虹,陈汉彬,2003,中国不同地理株白纹伊蚊细胞色素C氧化酶亚基Ⅰ基因序列比较,中国流行病学杂志,24(6):491-493)
Chen H.B.,and An J.Y.,eds.,2003,The Blackflies of China,Science Press,Beijing,China,pp.257-263(陈汉彬,安继尧,主编,2003,中国黑蝇(双翅目:蚋科),科学出版社,中国,北京,pp.257-263)
Chen H.B.,ed.,2016,Chinese Blackflies(Doptera:Simuliidae),Guizhou Science and Technology Publishing House,Guiyang,China,pp.389-390(陈汉彬,主编,2016,中国蚋科昆虫,贵州科技出版社,中国,贵阳,pp.389-390)
Cheng X.Y.,Zhou H.Z.,and Zhang G.X.,2000,Perspective of molecular biological techniques applied in insect systematics,Dongwu Fenlei Xuebao(Acta Zootaxonomica Sinica),25(2):121-133(成新跃,周红章,张广学,2000,分子生物学技术在昆虫系统学研究中的应用,动物分类学报,25(2):121-133)
He Y.X.,Chen X.D.,Fan S.S.,and Luo H.B.,2017,Molecular phylogeny based on mitochondrial COI gene for identification of simuliidae,Jinyinzuexue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(7):2826-2831(何艳霞,陈晓丹,樊莎莎,罗洪斌,2017,基于COI基因的分子系统发育分析在蚋科昆虫鉴定中的应用,基因组学与应用生物学,36(7):2826-2831)
Jiang L.,Yan J.,Wang T.T.,Guo Q.,Wang X.H.,and Yan C.C.,2015,A molecular phylogeny for some genera of the subfamily Chironominae(Diptera:Chironomidae)based on cytochrome oxidaseⅠgene,Tianjin shifan Daxue Xuebao(Journal of Tianjin Normal University),35(3):7-11,22(姜丽,闫娇,王婷婷,郭琴,王新华,闫春财,2015,基于COI基因的摇蚊亚科部分属的系统发育分析(双翅目:摇蚊科),天津师范大学学报,35(3):7-11,22)
Joy D.A.,and Conn J.E.,2001,Molecular and morphological phylogenetic analysis of an insular radiation in Pacific black flies(Simulium),Syst.Biol.,50(1):18-38
Krueger A.,and Hennings I.C.,2006,Molecular phylogenetics of black flies of the Simulium damnosum complex and cytophylogenetic implications,Mol.Phylogenet Evol.,39(1):83-90
Luo H.B.,Yang M.,and Chen H.B.,2010,Bioinformatics analysis of COI gene Simulium quinquestriatum,Zhongguo Jishengchongxue Yu Jishengchongbing Zazhi(Chinese Journal of Parasitology and Parasitic Diseases),28(6):473-475(罗洪斌,杨明,陈汉彬,2010,五条蚋细胞色素C氧化酶亚基Ⅰ(COI)基因的生物信息学分析,中国寄生虫学与寄生虫病杂志,28(6):473-475)
Pramual P.,Kuvangkadilok C.,Baimai V.,and Walton C.,2005Phylogeography of the black fly Simulium tani(Diptera Simuliidae)from Thailand as inferred from mt DNA sequences,Mol.Ecol.,14(13):3989-4001
Pramual P.,Wong P.K.,and Adler P.H.,2011,Cryptic biodiversity and phylogenetic relationships revealed by DNA barcoding of Oriental black flies in the subgenus Gomphostilbia(Diptera:Simuliidae),Genome,54(1):1-9
Sriphirom P.,Sopaladawan P.N.,Wongpakam K.,and Pramual P.2014,Molecular phylogeny of black flies in the Simulium tuberosum(Diptera:Simuliidae)species group in Thailand Genome,57(1):45-55
Xu X.,Yang M.,and Chen H.B.,2012,Sequence analysis of r D-NA-ITS1 of black flies from inner Mongolia,Guizhou Kexue(Guizhou Science),30(4):25-28(徐旭,杨明,陈汉彬,2012,内蒙古蚋类r DNA-ITS1序列分析,贵州科学,30(4):25-28)
Yang M.,Bi G.H.,and Chen H.B.,2004,Sequences analysis of nuclear ribosomal DNA ITS region of Simulium quinquestratum and S.bidentatun(Diptera:Simuliidae),Jishengchong Yu Yixuekunchong Xuebao(Acta Parasitologica et Medica Entomologica Sinica),11(4):219-222(杨明,毕光辉,陈汉彬,2004,五条蚋和双齿蚋nrDNA-ITS区序列分析(双翅目:蚋科),寄生虫与医学昆虫学报,11(4):219-222)
Yusseff-Vanegas S.Z.,and Agnarsson I.,2017,DNA-barcoding of forensically important blow flies(Diptera:Calliphoridae)in the Caribbean Region,Peer J.,25(5):e3516
Chen H.B.,and An J.Y.,eds.,2003,The Blackflies of China,Science Press,Beijing,China,pp.257-263(陈汉彬,安继尧,主编,2003,中国黑蝇(双翅目:蚋科),科学出版社,中国,北京,pp.257-263)
Chen H.B.,ed.,2016,Chinese Blackflies(Doptera:Simuliidae),Guizhou Science and Technology Publishing House,Guiyang,China,pp.389-390(陈汉彬,主编,2016,中国蚋科昆虫,贵州科技出版社,中国,贵阳,pp.389-390)
Cheng X.Y.,Zhou H.Z.,and Zhang G.X.,2000,Perspective of molecular biological techniques applied in insect systematics,Dongwu Fenlei Xuebao(Acta Zootaxonomica Sinica),25(2):121-133(成新跃,周红章,张广学,2000,分子生物学技术在昆虫系统学研究中的应用,动物分类学报,25(2):121-133)
He Y.X.,Chen X.D.,Fan S.S.,and Luo H.B.,2017,Molecular phylogeny based on mitochondrial COI gene for identification of simuliidae,Jinyinzuexue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(7):2826-2831(何艳霞,陈晓丹,樊莎莎,罗洪斌,2017,基于COI基因的分子系统发育分析在蚋科昆虫鉴定中的应用,基因组学与应用生物学,36(7):2826-2831)
Jiang L.,Yan J.,Wang T.T.,Guo Q.,Wang X.H.,and Yan C.C.,2015,A molecular phylogeny for some genera of the subfamily Chironominae(Diptera:Chironomidae)based on cytochrome oxidaseⅠgene,Tianjin shifan Daxue Xuebao(Journal of Tianjin Normal University),35(3):7-11,22(姜丽,闫娇,王婷婷,郭琴,王新华,闫春财,2015,基于COI基因的摇蚊亚科部分属的系统发育分析(双翅目:摇蚊科),天津师范大学学报,35(3):7-11,22)
Joy D.A.,and Conn J.E.,2001,Molecular and morphological phylogenetic analysis of an insular radiation in Pacific black flies(Simulium),Syst.Biol.,50(1):18-38
Krueger A.,and Hennings I.C.,2006,Molecular phylogenetics of black flies of the Simulium damnosum complex and cytophylogenetic implications,Mol.Phylogenet Evol.,39(1):83-90
Luo H.B.,Yang M.,and Chen H.B.,2010,Bioinformatics analysis of COI gene Simulium quinquestriatum,Zhongguo Jishengchongxue Yu Jishengchongbing Zazhi(Chinese Journal of Parasitology and Parasitic Diseases),28(6):473-475(罗洪斌,杨明,陈汉彬,2010,五条蚋细胞色素C氧化酶亚基Ⅰ(COI)基因的生物信息学分析,中国寄生虫学与寄生虫病杂志,28(6):473-475)
Pramual P.,Kuvangkadilok C.,Baimai V.,and Walton C.,2005Phylogeography of the black fly Simulium tani(Diptera Simuliidae)from Thailand as inferred from mt DNA sequences,Mol.Ecol.,14(13):3989-4001
Pramual P.,Wong P.K.,and Adler P.H.,2011,Cryptic biodiversity and phylogenetic relationships revealed by DNA barcoding of Oriental black flies in the subgenus Gomphostilbia(Diptera:Simuliidae),Genome,54(1):1-9
Sriphirom P.,Sopaladawan P.N.,Wongpakam K.,and Pramual P.2014,Molecular phylogeny of black flies in the Simulium tuberosum(Diptera:Simuliidae)species group in Thailand Genome,57(1):45-55
Xu X.,Yang M.,and Chen H.B.,2012,Sequence analysis of r D-NA-ITS1 of black flies from inner Mongolia,Guizhou Kexue(Guizhou Science),30(4):25-28(徐旭,杨明,陈汉彬,2012,内蒙古蚋类r DNA-ITS1序列分析,贵州科学,30(4):25-28)
Yang M.,Bi G.H.,and Chen H.B.,2004,Sequences analysis of nuclear ribosomal DNA ITS region of Simulium quinquestratum and S.bidentatun(Diptera:Simuliidae),Jishengchong Yu Yixuekunchong Xuebao(Acta Parasitologica et Medica Entomologica Sinica),11(4):219-222(杨明,毕光辉,陈汉彬,2004,五条蚋和双齿蚋nrDNA-ITS区序列分析(双翅目:蚋科),寄生虫与医学昆虫学报,11(4):219-222)
Yusseff-Vanegas S.Z.,and Agnarsson I.,2017,DNA-barcoding of forensically important blow flies(Diptera:Calliphoridae)in the Caribbean Region,Peer J.,25(5):e3516