高致病性猪繁殖与呼吸综合征病毒人工感染荣昌猪的试验研究
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摘要
为了评价中国地方猪种对高致病性猪繁殖与呼吸综合征病毒(HP-PRRSV)的易感性,使用1×106TCID50/m L的SCwhn09CD毒株人工感染中国地方品种荣昌猪,记录荣昌猪在试验期间的临床症状、体温变化、体重和死亡率。采用q PCR方法检测感染后第4、7、11和14天血清中的病毒拷贝数。病理切片观察感染后第14天肺部组织的损伤情况。结果显示:感染后荣昌猪表现为精神沉郁、饮食废绝、腹式呼吸、末梢皮肤发绀、持续高温、死亡、鼻腔内有黏性液体等典型猪繁殖与呼吸障碍综合征(PRRS)症状。感染后第3天直肠温度上升至40℃并一直持续到第14天,并在第12天达到峰值41.2℃。试验期间荣昌猪死亡率达66.7%。第4天血清中的病毒拷贝数达到峰值1.63×106拷贝/m L,随后缓慢下降至4.12×105拷贝/m L,但第4、7、11、14天间血清中的病毒拷贝数不存在显著性差异(P>0.05)。肺部组织出现了严重的支气管肺炎病变,肺泡壁显著增厚,致多数肺泡腔闭锁。
引文
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[12]Guo B,Lager K M,Schlink S N,et al.Chinese and Vietnamese strains of HP-PRRSV cause different pathogenic outcomes in United States high health swine[J].Virology,2013,446(1/2):238-250.
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[2]Prieto C,Suarez P,Bautista J M,et al.Semen changes in boars after experimental infection with porcine reproductive and respiratory syndrome(PRRS)virus[J].Theriogenology,1996,15;45(2):383-395.
[3]Hill H.Overview and history of Mystery Swine Disease(Swine infertility/respiratory syndrome)[C]//Proceedings of the Mystery Swine Disease Committee Meeting,Livestock Conservation Institute,Denver,Colorado,1990:29-31.
[4]Duan X,Nauwynck H J,Pensaert M B.Virus quantification and identification of cellular targets in the lungs and lymphoid tissues of pigs at different time intervals after inoculation with porcine reproductive and respiratory syndrome virus(PRRSV)[J].Vet Micro-biol,1997,56(1/2):9-19.
[5]Nathanson N,Ahmend R,Gonzailez-scarano F,et al.Viral Pathogenesis[M].Ahmed R,Morrison L A,Knipe D M.Viral persistence.Philadelphia:Lippincott-Raven,1997:181-206.
[6]Buddaert W,Van Reeth K,Pensaert M.In vivo and in vitro interferon(IFN)studies with the porcine reproductive and respiratory syndrome virus(PRRSV)[J].Adv Exp Med Biol,1998,440:461-467.
[7]Yoon K J,Wu L L,Zimmerman J J,et al.Antibody-dependent enhancement(ADE)of porcine reproductive and respiratory syndrome virus(PRRSV)infection in pigs[J].Viral Immunol,1996,9(1):51-63.
[8]Halbur P G,Rothschild M F,Thacker B J,et al.Differences in susceptibility of Duroc,Hampshire,and Meishan pigs to infection with a high virulence strain(VR2385)of porcine reproductive and respiratory syndrome virus(PRRSV)[J].J Anim Breed Genet,1998,115:181-189.
[9]Zhou Y S,Yang X,Wang H N.Molecular characterization of a complete genome and 12 Nsp2 genes of PRRSV of Southwestern China[J].Food Environ Virol,2012,4(3):102-114.
[10]Reiner G,Willems H,Pesch S.Variation in resistance to the porcine reproductive and respiratory syndrome virus(PRRSV)in pietrain and miniature pigs[J].J Anim Breed Genet,2010,127:100-106.
[11]Halbur P G,Paul P S,Meng X J,et al.A Comparative pathogenicity of nine US porcine reproductive and respiratory syndrome virus(PRRSV)isolates in a five-week-old cesarean-derived,colostrum-deprived pig model[J].J Vet Diagn Invest,1996,8(1):11-20.
[12]Guo B,Lager K M,Schlink S N,et al.Chinese and Vietnamese strains of HP-PRRSV cause different pathogenic outcomes in United States high health swine[J].Virology,2013,446(1/2):238-250.
[13]Linden I F A V D,Voermans J J M,Linde-Bril E M V D,et al.Virological kinetics and immunological responses to a porcine reproductive and respiratory syndrome virus infection of pigs at different ages[J].Vaccine,2003,21(17/18):1952-1957.