基于代谢组学研究新生儿黄疸患者及对蓝光治疗的反应
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摘要
目的:探讨新生儿黄疸患者光照前后血清代谢谱学变化,为进一步研究新生儿黄疸和光疗反应代谢谱学提高依据。方法:选取本院30例新生儿黄疸患者为新生儿黄疸组,25例健康新生儿为正常对照组。运用核磁共振(NMR)的代谢组学方法来研究生儿黄疸组光疗前后与正常对照组的血清代谢谱差异。数据通过多因素的主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)进行处理。结果:依据PCA和OPLS-DA模型鉴别,新生儿黄疸组与正常对照组比较有缬氨酸、肌醇、赖氨酸、亮氨酸等10种代谢物存在差异;新生儿黄疸组治疗前后有β-葡萄糖、α-葡萄糖、缬氨酸、酪氨酸等28种代谢物存在差异。结论:新生儿黄疸患者的代谢紊乱,且光疗对脂质、氨基酸和能量代谢的影响具有重要的作用。
Objective:To explore the changes of serum metabolic spectrum in neonatal jaundice patients before and after illumination,and to improve the basis for further study of neonatal jaundice and phototherapy response metabolic spectrum.Method:A total of 30 neonatal jaundice patients were selected as neonatal jaundice group and 25 healthy neonates as normal control group.The metabolomics method of nuclear magnetic resonance(NMR)was used to study the difference of serum metabolic spectrum between Jaundice group and normal control group before and after phototherapy.Data were processed by principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).Result:According to the identification of PCA and OPLS-DA models,there were 10 metabolites such as valine,inositol,lysine and leucine in the neonatal jaundice group compared with the normal control group,there were 28 metabolites such as β-glucose,α-glucose,valine and tyrosine in the neonatal jaundice group before and after treatment.Conclusion:Metabolic disorders in neonatal jaundice patients,and the effects of phototherapy on lipid,amino acid and energy metabolism play an important role.
Objective:To explore the changes of serum metabolic spectrum in neonatal jaundice patients before and after illumination,and to improve the basis for further study of neonatal jaundice and phototherapy response metabolic spectrum.Method:A total of 30 neonatal jaundice patients were selected as neonatal jaundice group and 25 healthy neonates as normal control group.The metabolomics method of nuclear magnetic resonance(NMR)was used to study the difference of serum metabolic spectrum between Jaundice group and normal control group before and after phototherapy.Data were processed by principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).Result:According to the identification of PCA and OPLS-DA models,there were 10 metabolites such as valine,inositol,lysine and leucine in the neonatal jaundice group compared with the normal control group,there were 28 metabolites such as β-glucose,α-glucose,valine and tyrosine in the neonatal jaundice group before and after treatment.Conclusion:Metabolic disorders in neonatal jaundice patients,and the effects of phototherapy on lipid,amino acid and energy metabolism play an important role.
引文
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[20]谢辉,谢艳,张若蒙,等.茵栀黄口服液对新生儿黄疸特征标记物影响的研究分析[J].吉林医学,2017,38(6):1052-1054.
[2]Riskin A,Cohen K,Kugelman A,et al.Influence of Changes in the Evaluation of Neonatal Jaundice[J].Am J Perinatol,2014,31(3):203-208.
[3]方衡,张爱华,周小航,等.基于代谢调控通路的京尼平苷干预阳黄证的作用靶标研究[J].世界科学技术-中医药现代化,2016,18(10):1697-1708.
[4]Stokowski L A.Fundamentals of Phototherapy for Neonatal Jaundice[J].Adv Neonatal Care,2011,11(5 Suppl):S10-21.
[5]Kale Y,Aydemir O,Celikü,et al.Effects of phototherapy using different light sources on oxidant and antioxidant status of neonates with jaundice[J].Early Hum Dev,2013,89(12):957-960.
[6]Sivanandan S,Chawla D,Misra S,et al.Effect of sling application on efficacy of phototherapy in healthy term neonates with nonhemolytic jaundice:a randomized conrolled trial[J].Indian Pediatr,2009,46(1):23-28.
[7]史秀云.基于LC-MS的胰腺癌血清代谢组学研究[D].哈尔滨:哈尔滨工业大学,2015.
[8]Romagnoli C,Barone G,Pratesi S,et al.Italian guidelines for management and treatment of hyperbilirubinaemia of newborn infants≥35 weeks’gestational age[J].Ital J Pediatr,2014,40(1):11.
[9]Duchnowski P,Hryniewiecki T,Kusmierczyk M,et al.Red Cell Distribution Width as a Prognostic Marker in Patients Undergoing Valve Surgery[J].Interact Cardiovasc Thorac Surg,2017,25(6):925-929.
[10]Izumi S I,Muano T,Mori A,et al.Common carotid artery stiffness,cardiovascular function and lipid metabolism after menopause[J].Life Sci,2006,78(15):1696-1701.
[11]蔡小蓉,杨建云,肖炳坤,等.茵陈五苓散对α-萘异硫氰酸酯致大鼠黄疸型肝损伤的血清代谢组学研究[J].中国临床药理学杂志,2018,34(7):848-851.
[12]Ichinomiya K,Inoue F,Koizumi A,et al.Problems with using total serum bilirubin as a criterion for phototherapy in extremely low-birthweight infants[J].Pediatr Int,2014,56(5):731-734.
[13]高源.希特林蛋白缺陷症致婴儿胆汁淤积性肝炎四例临床分析[J].中国综合临床,2016,32(5):467-470.
[14]Pazourek J.Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography[J].Carbohydr Res,2014,9(1):55-60.
[15]Franchini M,Targher G,Lippi G.Serum bilirubin levels and cardiovascular disease risk:a Janus Bifrons?[J].Adv Clin Chem,2010,50:47-63.
[16]浦仕彪.基于代谢组学的自身免疫性肝炎生物标志物筛查及中医药干预研究[D].成都:成都中医药大学,2013.
[17]Adamkin D H.Late preterm infants:severe hyperbilirubinemia and postnatal glucose homeostasis[J].J Perinatol,2009,29(Suppl 2):S12-17.
[18]朱洁.基于代谢组学的慢加急(亚急)性乙肝肝衰竭湿热证的证候研究[D].武汉:湖北中医药大学,2013.
[19]Yoshino S,Hamasaki S,Ishida S,et al.Characterization of the effect of serum bilirubin concentrations on coronary endothelial function via measurement of high-sensitivity C-reactive protein and high-density lipoprotein cholesterol[J].Heart Vessels,2013,28(2):157-165.
[20]谢辉,谢艳,张若蒙,等.茵栀黄口服液对新生儿黄疸特征标记物影响的研究分析[J].吉林医学,2017,38(6):1052-1054.