腹腔注射丹参注射液和血栓通注射液对大鼠睾丸扭转复位后caspase-3蛋白表达的影响
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摘要
目的:探讨腹腔注射丹参注射液和血栓通注射液对大鼠睾丸扭转复位后caspase-3蛋白表达的影响。方法:健康SD雄性大鼠,随机分为假手术组、生理盐水组、单次给药组和连续给药组。建立单侧睾丸扭转复位动物模型。术后6周取双侧睾丸切片行H-E染色;caspase-3蛋白免疫组织化学观察,免疫反应评分(IRS)进行半定量分析。结果:生理盐水组较假手术组caspase-3蛋白IRS明显增高,单次给药组和连续给药组caspase-3蛋白IRS较生理盐水组明显降低,连续给药组caspase-3蛋白IRS较单次给药组明显降低。结论:丹参注射液和血栓通注射液可能通过降低caspase-3蛋白的表达,减轻细胞凋亡,对睾丸缺血再灌注损伤具有明显的保护作用。
Objective:To investigate the effects of Danshen combined with Xueshuantong injection on the expression of caspase-3 following testicular torsion/detorsion in rats. Methods : Healthy male SD rats were randomly divided into singleinjection group, continuous-injection group, sham group and saline group. The animal model of unilateral testicular torsion reduction was established. The bilateral testicular specimens were taken and dyed by hematoxylin-eosin six weeks after the modelling. Caspase-3 was observed by immunohistochemistry and immunoreactive scoring(IRS) for semi-quantitative analysis. Results: Compared with the sham group, the IRS of caspase-3 in the saline group was significantly increased.Compared with the saline group, the IRS of caspase-3 in the single-injection and continuous-injection groups were significantly increased. Compared with the single-injection group, the IRS of caspase-3 in continuous-injection group was significantly reduced. Conclusion: Danshen combined with Xueshuantong injection can obviously reduce the expression of caspase-3 to inhibit the apoptosis and can significantly protect the testicular ischemia-reperfusion injury in rats.
Objective:To investigate the effects of Danshen combined with Xueshuantong injection on the expression of caspase-3 following testicular torsion/detorsion in rats. Methods : Healthy male SD rats were randomly divided into singleinjection group, continuous-injection group, sham group and saline group. The animal model of unilateral testicular torsion reduction was established. The bilateral testicular specimens were taken and dyed by hematoxylin-eosin six weeks after the modelling. Caspase-3 was observed by immunohistochemistry and immunoreactive scoring(IRS) for semi-quantitative analysis. Results: Compared with the sham group, the IRS of caspase-3 in the saline group was significantly increased.Compared with the saline group, the IRS of caspase-3 in the single-injection and continuous-injection groups were significantly increased. Compared with the single-injection group, the IRS of caspase-3 in continuous-injection group was significantly reduced. Conclusion: Danshen combined with Xueshuantong injection can obviously reduce the expression of caspase-3 to inhibit the apoptosis and can significantly protect the testicular ischemia-reperfusion injury in rats.
引文
[1]Li Y,Chopp M,Jiang N,et al.Induction of DNA fragmentationafter 10 to 120 minutes of focal cerebral ischemia in rats[J].Stroke,1995,26(7):1257-1258.
[2]杨斐,杨超玲,余雯文,等.丹血通注射液对大鼠睾丸扭转复位后的保护作用[J].中华男科学杂志,2015,21(9):828-832.
[3]Turner T T,Lysiak J J,Shannon J D,et al.Testicular torsion alters the presense of specific proteins in the mouse testics as well as the phosphorylation status of specific proteins[J].J Androl,2006,27(2):285.
[4]滕若冰,喻爱芳,莫发荣.无精子症睾丸组织中survivin、Caspase-3的表达及意义[J].山东医药,2009,49(46):9-10.
[5]Miyamoto H,Doita M,Nishida K,et al.Effects of cyclic mechanical stress on the production of inflammatory agents by nucleus pulposus and anulus fibrosus derived cells in vitro[J].Spine,2006,31(1):4-9.
[6]Kaur S,Jaggi A S,Singh N.Molecular aspects of ischaemic postconditioning[J].Fund Clin Pharmacol,2009,23(5):521-536.
[7]薛晶,冯加纯,杨艺敏,等.脑缺血再灌注后炎症和自由基作用的实验研究[J].中国老年学杂志,2009,29(6):644-646.
[8]Saikumar P,Dong Z,Weniberg J M,et al.Mechanisms of cell death in hypoxia/reoxygenation injury[J].Oncogene,1998,17(25):3341-3349.
[9]Cosulich S C,Savory P J,Clarke P R.Bcl-2 regulates amplification of caspase activation by cytochrome[J].Curr Biol,1999,9(3):147-150.
[10]Faubel S,Edelstein C L.Caspase as drug targets in ischemic organ injury[J].Curr Drug Targets Immune Endocr Metabol Disord,2005,5(3):269-287.
[11]Ateau Roesch O,Argaud L,Ovize M.Mitochondrial permeability transition pore and postconditioning[J].Cardiovasc Res,2006,70(2):264-273.
[12]徐渊,殷金龙,吴斌.五子衍宗复方改善Sertoli细胞氧化应激损伤的作用[J].现代中西医结合杂志,2013,22(15):1615-1626.
[13]马丙祥,董宠凯.丹参的药理作用研究新进展[J].中国药房,2014,25(7):663-665.
[14]崔红晶,安长新,陈东,等.三七总苷对大鼠自身免疫性睾丸炎的拮抗作用[J].解剖学杂志,2008,31(1):19-21.
[15]陈锐娥,申东艳,李鹏,等.丹参三七药对研究[J].世界科学技术:中医药现代化,2012,14(2):1342-1348.
[16]Zhou L,Bondy S C,Jian L,et al.TanshinoneⅡA attenuates the cerebral ischemic injury-induced increase in levels of GFAP and of caspase-3 and-8[J].Neuroscience,2015,288(5):105-111.
[17]倪世容,徐正衸,王鑫.三七总皂甙降低兔肺缺血再灌注损伤和抑制细胞凋亡[J].基础医学与临床,2012,32(2):170-174.
[2]杨斐,杨超玲,余雯文,等.丹血通注射液对大鼠睾丸扭转复位后的保护作用[J].中华男科学杂志,2015,21(9):828-832.
[3]Turner T T,Lysiak J J,Shannon J D,et al.Testicular torsion alters the presense of specific proteins in the mouse testics as well as the phosphorylation status of specific proteins[J].J Androl,2006,27(2):285.
[4]滕若冰,喻爱芳,莫发荣.无精子症睾丸组织中survivin、Caspase-3的表达及意义[J].山东医药,2009,49(46):9-10.
[5]Miyamoto H,Doita M,Nishida K,et al.Effects of cyclic mechanical stress on the production of inflammatory agents by nucleus pulposus and anulus fibrosus derived cells in vitro[J].Spine,2006,31(1):4-9.
[6]Kaur S,Jaggi A S,Singh N.Molecular aspects of ischaemic postconditioning[J].Fund Clin Pharmacol,2009,23(5):521-536.
[7]薛晶,冯加纯,杨艺敏,等.脑缺血再灌注后炎症和自由基作用的实验研究[J].中国老年学杂志,2009,29(6):644-646.
[8]Saikumar P,Dong Z,Weniberg J M,et al.Mechanisms of cell death in hypoxia/reoxygenation injury[J].Oncogene,1998,17(25):3341-3349.
[9]Cosulich S C,Savory P J,Clarke P R.Bcl-2 regulates amplification of caspase activation by cytochrome[J].Curr Biol,1999,9(3):147-150.
[10]Faubel S,Edelstein C L.Caspase as drug targets in ischemic organ injury[J].Curr Drug Targets Immune Endocr Metabol Disord,2005,5(3):269-287.
[11]Ateau Roesch O,Argaud L,Ovize M.Mitochondrial permeability transition pore and postconditioning[J].Cardiovasc Res,2006,70(2):264-273.
[12]徐渊,殷金龙,吴斌.五子衍宗复方改善Sertoli细胞氧化应激损伤的作用[J].现代中西医结合杂志,2013,22(15):1615-1626.
[13]马丙祥,董宠凯.丹参的药理作用研究新进展[J].中国药房,2014,25(7):663-665.
[14]崔红晶,安长新,陈东,等.三七总苷对大鼠自身免疫性睾丸炎的拮抗作用[J].解剖学杂志,2008,31(1):19-21.
[15]陈锐娥,申东艳,李鹏,等.丹参三七药对研究[J].世界科学技术:中医药现代化,2012,14(2):1342-1348.
[16]Zhou L,Bondy S C,Jian L,et al.TanshinoneⅡA attenuates the cerebral ischemic injury-induced increase in levels of GFAP and of caspase-3 and-8[J].Neuroscience,2015,288(5):105-111.
[17]倪世容,徐正衸,王鑫.三七总皂甙降低兔肺缺血再灌注损伤和抑制细胞凋亡[J].基础医学与临床,2012,32(2):170-174.