喹诺酮信号分子和环丙沙星诱导铜绿假单胞菌对环丙沙星最低抑菌浓度影响的比较
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摘要
目的探讨铜绿假单胞菌经喹诺酮信号分子(PQS)和环丙沙星2种诱导方案诱导后对环丙沙星最低抑菌浓度(MIC)的影响。方法收集临床分离的对环丙沙星敏感的铜绿假单胞菌,PQS以10,40,80μmol·L~(-1)3种诱导浓度共诱导5 d,环丙沙星以0.5×MIC,2×MIC和4×MIC 3种诱导浓度共诱导5 d,用琼脂倍比稀释法测定PQS和环丙沙星诱导前后环丙沙星的MIC值。同一诱导方案诱导前后对环丙沙星的MIC值用重复测量方差分析方法统计分析;2种诱导方案MIC值的比较用配对t检验。结果共得到12株对环丙沙星敏感的铜绿假单胞菌,其中包含1株质控菌。PQS的诱导浓度与诱导时间之间有交互作用(P<0.001),环丙沙星的诱导浓度与诱导时间之间有交互作用(P<0.05),2种诱导方案对环丙沙星MIC值的影响差异均有统计学意义(均P<0.05)。结论不同PQS诱导浓度下铜绿假单胞菌对环丙沙星MIC值随诱导时间的延长其变化的趋势不同;不同环丙沙星诱导浓度下MIC值随诱导时间的延长均呈增大的趋势,2种诱导方案对环丙沙星MIC值的影响不同。
Objective To investigate minimal inhibitory concentration( MIC) of ciprofloxacin against Pseudomonas aeruginosa induced by Pseudomonas quinolone signal( PQS) and ciprofloxacin in vitro. Methods Clinical isolates of Pseudomonas aeruginosa sensitive to ciprofloxacin were collected and then induced ciprofloxacin with three concentrations of0. 5 × MIC,2 × MIC and 4 × MIC,and PQS with three concentrations of10,40,80 μmol·L~(-1),respectively for five days. The agar dilution method was used to measure MICs of all strains before and after inductions to ciprofloxacin. The MICs to ciprofloxacin before and after inductions of the same induction scheme were analysed by repeated measures analysis of variance and Paired t-test was used to compare the MICs of two induced schemes. Results Twelve clinical isolates of Pseudomonas aeruginosa sensitive to ciprofloxacin were obtained,among them one was used as the quality control strain included. There had interaction between induction time and induction concentrations of PQS or ciprofloxacin( P < 0. 001 or P < 0. 05). MICs of strains to ciprofloxacin of two induced schemes had statistically significant difference( P < 0. 05). Conclusion Under different concentrations of PQS,the trend of MIC values ofciprofloxacin to Pseudomonas aeruginosa varied by induction time. Under different concentrations of ciprofloxacin,MIC values tended to increase with the prolongation of induction time. The effects of two induction schemes on MIC of ciprofloxacin were different.
Objective To investigate minimal inhibitory concentration( MIC) of ciprofloxacin against Pseudomonas aeruginosa induced by Pseudomonas quinolone signal( PQS) and ciprofloxacin in vitro. Methods Clinical isolates of Pseudomonas aeruginosa sensitive to ciprofloxacin were collected and then induced ciprofloxacin with three concentrations of0. 5 × MIC,2 × MIC and 4 × MIC,and PQS with three concentrations of10,40,80 μmol·L~(-1),respectively for five days. The agar dilution method was used to measure MICs of all strains before and after inductions to ciprofloxacin. The MICs to ciprofloxacin before and after inductions of the same induction scheme were analysed by repeated measures analysis of variance and Paired t-test was used to compare the MICs of two induced schemes. Results Twelve clinical isolates of Pseudomonas aeruginosa sensitive to ciprofloxacin were obtained,among them one was used as the quality control strain included. There had interaction between induction time and induction concentrations of PQS or ciprofloxacin( P < 0. 001 or P < 0. 05). MICs of strains to ciprofloxacin of two induced schemes had statistically significant difference( P < 0. 05). Conclusion Under different concentrations of PQS,the trend of MIC values ofciprofloxacin to Pseudomonas aeruginosa varied by induction time. Under different concentrations of ciprofloxacin,MIC values tended to increase with the prolongation of induction time. The effects of two induction schemes on MIC of ciprofloxacin were different.
引文
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[5]NAKAJIMA A,SUGIMOTO Y,YONEYAMA H,et al.High-level fluoroquilone resistance in Pseudomonas aeruginosa due to interplay of the Mex AB-Opr M efflux pump and the DNA gyrase mutation[J].Microbiol Immunol,2002,46(6):391-395.
[6]郑红达,周丽曼,唐艺丹,等.喹诺酮信号系统研究进展[J].现代生物医学进展,2014,14(11):2170-2172.
[2]CLINICALAND LABORATORY STANDARD INSTITUTE.Performance standards for antimicrobial susceptibility testing.Twentyseventh information supplement[S].Pennsylvania:CLSI,2017:M100-S27.
[3]HUSSLER S,BECKER T.The Pseudomonas quinolone signal(PQS)balances life and death in Pseudomonas aeruginosa,populations[J/OL].PLo S Pathog,2008,4(9):e1000166.2008-09-26[2017-12-10].http://dx.plos.org/10.1371/journal.ppat.1000166.
[4]TOYOFUKU M,NAKAJIMA-KAMBE T,UCHIYAMA H,et al.The effect of a cell-to-cell communication molecule,Pseudomonas quinolone signal(PQS),produced by P.aeruginosa on other bacterial species[J].Microbes Environ,2010,25(1):1-7.
[5]NAKAJIMA A,SUGIMOTO Y,YONEYAMA H,et al.High-level fluoroquilone resistance in Pseudomonas aeruginosa due to interplay of the Mex AB-Opr M efflux pump and the DNA gyrase mutation[J].Microbiol Immunol,2002,46(6):391-395.
[6]郑红达,周丽曼,唐艺丹,等.喹诺酮信号系统研究进展[J].现代生物医学进展,2014,14(11):2170-2172.