表没食子儿茶素没食子酸酯对病毒性心肌炎小鼠的心肌保护作用
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摘要
目的探讨表没食子儿茶素没食子酸酯(EGCG)对病毒性心肌炎小鼠的心肌保护作用。方法将45只雄性BALA/C小鼠随机分为正常组、对照组及EGCG组,每组15只。对照组、EGCG组小鼠腹腔注射0.1 m L CVB3病毒液。接种成功后,EGCG组予以EGCG 100 mg/(kg·d)灌胃处理,对照组予以等量的生理盐水灌胃。正常组小鼠腹腔注射等量的生理盐水。饲养1周后,摘除眼球取血检测肌酸激酶同工酶(CK-MB)、心肌肌钙蛋白T(c TnT)的活性,然后处死动物取出心脏,HE染色观察心肌细胞形态学改变。结果 EGCG组小鼠的生存率显著高于对照组;对照组血清CK-MB、c Tn T的活性及心肌病理评分显著高于EGCG组。结论 EGCG对病毒性心肌炎具有一定的保护作用。
Objective To investigate the role of epigallocatechin gallate(EGCG) in protecting myocardium of rats with viral myocarditis.Methods A total of 45 male BALA/C rats were randomly divided into normal group,control group and EGCG group,with 15 rats in each group.Rats in the control group and EGCG group were intraperitoneally injected with 0.1 m L CVB3 viral fluid.After successful inoculation,the EGCG group was treated with 100 mg/kg EGCG per day,while the control group was treated with the same amount of saline.Normal rats were injected with the same amount of saline.After feeding for one week,the eyeball blood was removed to detect the activity of creatine kinase isoenzyme(CK-MB) and cardiac troponin T(cTnT).Then the heart was taken out and the morphological changes of cardiac myocytes were observed by HE staining.Results The survival rate of rats in the EGCG group was obviously higher than that in the control group,and the serum CK-MB,cTnT activity and myocardial pathological score in the control group were obviously higher than those in the EGCG group.Conclusion EGCG has a certain protective effect on viral myocarditis.
Objective To investigate the role of epigallocatechin gallate(EGCG) in protecting myocardium of rats with viral myocarditis.Methods A total of 45 male BALA/C rats were randomly divided into normal group,control group and EGCG group,with 15 rats in each group.Rats in the control group and EGCG group were intraperitoneally injected with 0.1 m L CVB3 viral fluid.After successful inoculation,the EGCG group was treated with 100 mg/kg EGCG per day,while the control group was treated with the same amount of saline.Normal rats were injected with the same amount of saline.After feeding for one week,the eyeball blood was removed to detect the activity of creatine kinase isoenzyme(CK-MB) and cardiac troponin T(cTnT).Then the heart was taken out and the morphological changes of cardiac myocytes were observed by HE staining.Results The survival rate of rats in the EGCG group was obviously higher than that in the control group,and the serum CK-MB,cTnT activity and myocardial pathological score in the control group were obviously higher than those in the EGCG group.Conclusion EGCG has a certain protective effect on viral myocarditis.
引文
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[14]胡孝彬,向小节,郭晓聪,等.高敏肌钙蛋白T对儿童病毒性心肌炎诊断价值探讨[J].检验医学,2014,29(4):307-311.
[15]He X R,Gao B,Zhou L,et al.Green tea polyphenol epigallocatechin-3-gallate-alleviated coxsackievirus B3-induced myocarditis through inhibiting viral replication but not through inhibiting inflammatory responses[J].J Cardiovasc Pharmacol,2017,69(1):41-47.
[2]Hendry R G,Bilawchuk L M,Marchant D J.Targeting matrix metalloproteinase activity and expression for the treatment of viral myocarditis[J].J of Cardiovasc Trans Res,2014,7(2):212-225.
[3]王振先,郝芳之,徐成伟,等.复合蛋白锌对病毒致小鼠心肌损伤的保护作用[J].山东大学学报:医学版,2004,42(1):106-109.
[4]Khan N,Mukhtar H.Tea polyphenols in promotion of human health[J].Nutrients,2018,11(1):E39-E45.
[5]Cen Z,Guo Y,Kong Q,et al.IL-10-producing B cells involved in the pathogenesis of Coxsackie virus B3-induced acute viral myocarditis[J].International Journal of Clinical&Experimental Pathology,2015,8(1):830-835.
[6]Chacko S M,Thambi P T,Kuttan R,et al.Beneficial effects of green tea:a literature review[J].Chin Med,2010,5:13-19.
[7]Yang C S,Wang H.Cancer preventive activities of tea catechins[J].Molecules,2016,21(12):E1679-E1685.
[8]Xu J,Xu Z,Zheng W M.A review of the antiviral role of green tea catechins[J].Molecules,2017,22(8):E1337-E1341.
[9]Ide K,Kawasaki Y,Kawakami K,et al.Anti-influenza virus effects of catechins:a molecular and clinical review[J].Curr Med Chem,2016,23(42):4773-4783.
[10]Xu J,Wang J,Deng F,et al.Green tea extract and its major component epigallocatechin gallate inhibits hepatitis B virus in vitro[J].Antiviral Res,2008,78(3):242-249.
[11]Calland N,Albecka A,Belouzard S,et al.Epigallocatechin-3-gallate is a new inhibitor of hepatitis C virus entry[J].Hepatology,2012,55(3):720-729.
[12]Lyu S Y,Rhim J Y,Park W B.Antiherpetic activities of flavonoids against herpes simplex virus type 1(HSV-1)and type2(HSV-2)in vitro[J].Arch Pharm Res,2005,28(11):1293-1301.
[13]钱净,李雪,杨丽琼,等.常见心肌损伤标志物在急性心肌梗死诊断中的应用价值[J].检验医学与临床,2019,16(1):31-34.
[14]胡孝彬,向小节,郭晓聪,等.高敏肌钙蛋白T对儿童病毒性心肌炎诊断价值探讨[J].检验医学,2014,29(4):307-311.
[15]He X R,Gao B,Zhou L,et al.Green tea polyphenol epigallocatechin-3-gallate-alleviated coxsackievirus B3-induced myocarditis through inhibiting viral replication but not through inhibiting inflammatory responses[J].J Cardiovasc Pharmacol,2017,69(1):41-47.