翘嘴鲌精子生理特性及超低温冷冻的研究
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摘要
采用试验生态——显微观察方法对翘嘴鲌精子的生理特性进行了研究,并用两步降温法超低温冷冻保存翘嘴鲌精子,筛选出适宜的稀释液与抗冻剂配方。结果表明,翘嘴鲌适宜的盐度范围为0～0.4%,pH值为5.5～8.0时,精子激活率较高,其中pH值为5.5时,精子运动时间与寿命最长;翘嘴鲌精子在4℃条件下保存84 h仍有48.33%的激活率,在25℃条件下保存84 h时精子死亡。以D-15为稀释液、10%乙二醇(EG)为抗冻剂,采用两步降温法超低温冷冻保存翘嘴鲌精子,解冻后精子活力最高。
引文
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[3]朱其广,王健,杨少荣,等.三峡库区木洞江段翘嘴鲌早期生长特征研究[J].水生生物学报,2015,39(5):983-988.
[4]覃亮,熊邦喜,吕光俊.徐家河水库翘嘴鲌的个体生殖力[J].应用生态学报,2009,20(8):1952-1957.
[5]崔中倩,宋洪建,尹海富.翘嘴鲌营养需要研究进展[J].中国饲料,2012(23):12-15.
[6]宋林,樊启学,胡培培,等.饲料蛋能比对翘嘴鲌幼鱼生长性能、肠道和肝胰脏消化酶活性的影响[J].动物营养学报,2013,25(7):1480-1487.
[7]王贵英,张涵,李清,等.黑尾近红鲌精子低温保存方法研究与应用[J].淡水渔业,2016,46(6):3-7,32.
[8]王贵英,李清,祝东梅,等.黑尾近红鲌精子活力研究[J].湖北农业科学,2016,55(8):2062-2065.
[9]黄辨非,罗静波.氯化钠溶液对兴国红鲤精子活力的影响[J].湖北农学院学报,2000,20(1):62-64.
[10]赵维信,姜仁良,刘修英,等.几种鲤科鱼类精子和胚胎冷冻损伤的扫描电镜研究[J].淡水渔业,1992(5):3-5.
[11]陈松林,刘宪亭,鲁大椿,等.鲢、鲤、团头鲂和草鱼精液冷冻保存的研究存[J].动物学报,1992,38(4):413-424.
[12]丁淑燕,严维辉,郝忱,等.兴国红鲤精液超低温冷冻保存及效果分析[J].江苏农业科学,2016,44(2):277-279.
[13]Alavia S M H,cosson J.Sperm motility in fishes.Ⅰ.Effects of temperature and p H:a review[J].Cell Biology International,2005(29):101-110.
[14]Chantzaropoulos A,Nathanailides C,Kokokiris L,et al.A brief exposure to low pH prior to refrigerated storage reduces the motility and viability of goldfish sperm(Carassius auratus,Linnaeus,1758)[J].Journal of Applied Ichthyology,2015(31):89-93.
[15]王晓爱,杨君兴,陈小勇,等.软鳍新光唇鱼精子的超低温冷冻保存[J].动物学研究,2012,33(3):283-289.
[16]王晓爱,杨君兴,陈小勇,等.4种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响[J].水生态学杂志,2012,33(5):88-93.
[17]孟令寀,温海深,韩龙江.大泷六线鱼精液超低温冷冻保存效果分析[J].现代农业科技,2015(22):262-264.
[18]Lahnsteiner F B B,Horvath A E A.Cryopreservation of spermatozoa in cyprinid fishes[J].Theriogenology,2000(54):1477-1498.
[19]Chew P C,Hassan R,Rashid Z A,et al.The current status of sperm cryopreservation of the endangered Probarbus jullieni(Sauvage)in Malaysia[J].Journal of Applied Ichthyology,2010,26(5):797-805.
[20]Dietrich G J,Wolnicki J,Slowinska M,et al.Short-term storage and cryopreservation of lake minnow[Eupallasella percnurus(Pallas,1814)]sperm[J].Journal of Applied Ichthyology,2015,31(1,SI):75-78.
[21]Gilmore J A,Liu J,Gao D Y,et al.Determination of optimal cryoprotectants and procedures for their addition and removal from human spermatozoa[J].Human Reproduction,1997,12(1):112-118.