EDTA增强Cr(VI)共存时Burkholderia cepacia降解孔雀绿的效率
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摘要
重金属共存严重抑制了微生物降解染料的效率,本研究拟采用EDTA螯合Cr提高Burkholderia cepacia C09G降解复合污染中孔雀绿的效率.实验结果表明,0.1 mmol·L~(-1)孔雀绿单独存在条件下,24 h的生物降解率达到96.2%;然而,在0.5 mmol·L~(-1)Cr(VI)共存条件下,60 h的降解率仅为6.7%.当加入0.5 mmol·L~(-1)EDTA螯合剂后,60 h时孔雀绿的降解率提高到18.8%.当EDTA浓度为0.5 mmol·L~(-1)时,最佳螯合Cr(VI)的浓度为0.7 mmol·L~(-1),此时,60 h后孔雀绿的降解率达到35.3%,对Cr(VI)的吸附率为24.6%.此外,通过EDS、SEM、XPS分析证明,EDTA可以减小Cr(VI)的毒性,Burkholderia cepacia C09G可将吸附的Cr(VI)还原为Cr(III).
The biodegradation efficiencies of dyes were inhibited in the presence of heavy metals.In this study,EDTA was used as a Cr chelating agent to increase the degradation efficiency of malachite green(MG)by Burkholderia cepacia C09G.The results show that the biodegradation efficiency of MG at0.1 mmol·L~(-1)was 96.2%at 24 h.However,the MG removal efficiency was only 6.7%at 60 h in the presence of 0.5 mmol·L~(-1)of Cr(VI),while 18.8%was obtained if amended with 0.5 mmol·L~(-1)of EDTA.When concentration of EDTA was 0.5 mmol·L~(-1),the optimal chelating concentration of Cr(VI)was 0.7 mmol·L~(-1),where the degradation efficiency of MG was 35.3%and adsorption efficiency of Cr(VI)was 24.6%.In addition,the EDS,SEM and XPS analysis confirm that the adsorbed Cr(VI)was reduced to Cr(III)by Burkholderia cepacia C09G and the toxicity of Cr(VI)alleviated due to the chelation of EDTA.
The biodegradation efficiencies of dyes were inhibited in the presence of heavy metals.In this study,EDTA was used as a Cr chelating agent to increase the degradation efficiency of malachite green(MG)by Burkholderia cepacia C09G.The results show that the biodegradation efficiency of MG at0.1 mmol·L~(-1)was 96.2%at 24 h.However,the MG removal efficiency was only 6.7%at 60 h in the presence of 0.5 mmol·L~(-1)of Cr(VI),while 18.8%was obtained if amended with 0.5 mmol·L~(-1)of EDTA.When concentration of EDTA was 0.5 mmol·L~(-1),the optimal chelating concentration of Cr(VI)was 0.7 mmol·L~(-1),where the degradation efficiency of MG was 35.3%and adsorption efficiency of Cr(VI)was 24.6%.In addition,the EDS,SEM and XPS analysis confirm that the adsorbed Cr(VI)was reduced to Cr(III)by Burkholderia cepacia C09G and the toxicity of Cr(VI)alleviated due to the chelation of EDTA.
引文
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Nandi B,Goswami A,Purkait M.2009.Adsorption characteristics of brilliant green dye on Kaolin[J].Journal of Hazardous Materials,161(1):387-395
Campbell C D,Hird M,Lumsdon D G,et al.2000.The effect of EDTA and fulvic acid on Cd,Zn,and Cu toxicity to a bioluminescent construct(p UCD607)of Escherichia coli[J].Chemosphere,40:319-325
Chaudhari A U,Tapase S R,Markad V L,et al.2013.Simultaneous decolorization of reactive Orange M2R dye and reduction of chromate by Lysinibacillus sp.KMK-A[J].Journal of Hazardous Materials,262:580-588
Chen C H,Chang C F,Liu S M.2010.Partial degradation mechanisms of malachite green and methyl violet B by Shewanella decolorationis NTOU1 under anaerobic conditions[J].Journal of Hazardous Materials,177:281-289
甘莉,周凤妃,陈祖亮.2014.固定化Burkholderia vietnamiensis C09V的生物材料同时去除结晶紫和Cr(VI)[J].环境科学学报,34(1):87-91
Grcman H,Velikonja-Bolta S,Vodnik D,et al.2001.EDTA enhanced heavy metal phytoextraction:metal accumulation,leaching,and toxicity[J].Plant Soil,235:105-114
鞠峰,胡勇有,程建华,等.2011.铁屑内电解法处理EDTA溶液中络合铜离子[J].环境科学学报,31(5):897-904
Gopinath K P,Kathiravan M N,Srinivasan R,et al.2011.Evaluation and elimination of inhibitory effects of salts and heavy metal ions on biodegradation of Congo red by Pseudomonas sp.mutant[J].Bioresource Technology,102(4):3687-3693
Kinoshita H,Sohma Y,Ohtake F,et al.2013.Biosorption of heavy metals by lactic acid bacteria and identification of mercury binding protein[J].Research in Microbiology,164:701-709
Kim S Y,Jin M R,Chung C H,et al.2015.Biosorption of cationic basic dye and cadmium by the novel biosorbent Bacillus catenulatus JB-022strain[J].Journal of Bioscience and Bioengineering,119(4):433-439
Lira-Silva E,Ramírez-Lima I S,Olín-Sandoval V,et al.2011.Removal,accumulation and resistance to chromium in heterotrophic Euglena gracilis[J].Journal of Hazardous Materials,193:216-224
Maszenan A M,Liu Y,Ng W J.2011.Research review paper:Bioremediation of wastewaters with recalcitrant organic compounds and metals by aerobic granules[J].Biotechnology Advances,29:111-123
Nandi B,Goswami A,Purkait M.2009.Adsorption characteristics of brilliant green dye on Kaolin[J].Journal of Hazardous Materials,161(1):387-395