孕哺期氧化乳蛋白饮食对子代小鼠肠道氧化还原状态的影响
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摘要
目的:研究孕哺期氧化乳蛋白饮食对子代小鼠肠道氧化还原状态的影响。方法:通过不同处理方法(加热、HClO、H_2O_2-Cu)对全脂乳进行氧化修饰,测定其羰基、巯基含量。雌雄鼠合笼成功即开始饲喂氧化乳蛋白日粮3周,产后母乳喂养,孕哺期间母鼠饲料相同,3周断乳后即测定子代小鼠肠道的抗氧化及氧化损伤指标。结果:乳蛋白经氧化修饰后,羰基含量显著上升(p<0.05),巯基含量显著下降(p<0.05)。孕哺期饲喂氧化乳蛋白日粮,子代小鼠肠道氧化还原状态失衡,与空白对照组相比,氧化修饰组子代小鼠肠道抗氧化酶活力(CAT、SOD)和总抗氧化能力(T-AOC)均呈现不同程度的下降(p<0.05),脂质过氧化物丙二醛(MDA)、4-羟基壬烯酸(HNE)在肠道内大量累积。结论:经不同的氧化修饰,乳蛋白发生不同程度的氧化,导致乳蛋白理化性质和结构发生变化,孕哺期饲喂氧化乳蛋白日粮,引起子代小鼠肠道氧化应激,降低其抗氧化防御能力。
Objective: During processing and storage, dairy protein can be easily oxidized. To investigate the intestinal redox state effects of offspring mice which were treated with oxidation dairy protein during pregnancy and lactation. Methods: We performed oxidized dairy protein modified respectively by heat,HClO and H_2O_2-Cu, determination of the variation on carbonyl content, free thiol group of dairy protein in vitro. 6 weeks feeding of oxidized dairy protein as basal protein caused oxidative stress on intestinal of offspring mice during pregnancy and lactation. Results: After oxidation modified, the carbonyl content of dairy protein was increased(p<0.05), while free thiol group content decreased(p<0.05). After weaning,in intestinal of offspring mice, activities of antioxidant enzymes(SOD, CAT) and T-AOC significantly decreased(p<0.05), moreover, the dates displayed the accumulation of MDA, HNE in intestinal of offspring mice. Conclusion: Oxidation would lead to changes in physical and chemical properties of dairy protein,oxidized dairy protein impairs antioxidant defense system in intestinal of offspring mice.
Objective: During processing and storage, dairy protein can be easily oxidized. To investigate the intestinal redox state effects of offspring mice which were treated with oxidation dairy protein during pregnancy and lactation. Methods: We performed oxidized dairy protein modified respectively by heat,HClO and H_2O_2-Cu, determination of the variation on carbonyl content, free thiol group of dairy protein in vitro. 6 weeks feeding of oxidized dairy protein as basal protein caused oxidative stress on intestinal of offspring mice during pregnancy and lactation. Results: After oxidation modified, the carbonyl content of dairy protein was increased(p<0.05), while free thiol group content decreased(p<0.05). After weaning,in intestinal of offspring mice, activities of antioxidant enzymes(SOD, CAT) and T-AOC significantly decreased(p<0.05), moreover, the dates displayed the accumulation of MDA, HNE in intestinal of offspring mice. Conclusion: Oxidation would lead to changes in physical and chemical properties of dairy protein,oxidized dairy protein impairs antioxidant defense system in intestinal of offspring mice.
引文
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[15]李婷婷,彭军.次氯酸介导的氧化应激及其病理生理学意义[J].中国药理学与毒理学杂志,2010,24(4):307-310.
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[17]Li ZL,Shi Y,DingY,et al.Dietary oxidized tyrosine(TOP)stimulates TGF-β1-induced extracellular matrix production via the JNK/p38 signaling pathway in rat kidneys[J].Amino Acids,2017,49(2):241-260.
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[2]Dalsgaard TK,Nielsen J H,Brown B E,et al.Dityrosine,3,4-dihydroxyphenylalanine(DOPA),and radical formation from tyrosine residues on milk proteins with globular and flexible structuresas a result of riboflavinmediated photo-oxidation[J].Journal of Agricultural and Food Chemistry,2011,59:7939-7947.
[3]莫玲,乐国伟,施用晖.干燥方法对乳蛋白氧化及机体氧化还原状态的影响[J].食品工业科技,2013,(8):170-186.
[4]Xie F,Sun S,Xu A,et al.Advanced oxidation protein products induce intestine epithelial cell death through a redox-dependent,c-jun N-terminal kinase and poly(ADP-ribose)polymerase-1-mediated pathway[J].Cell Death Disease,2014,(5).
[5]Soladoye O P,Juárez M L,Aalhus J L,et al.Protein oxidation in processed meat:Mechanisms and potential implications on human health[J].Comprehensive Reviews in Food Science and Food Safety,2015,14:106-122.
[6]Li ZL,Mo L,Le G,et al.Oxidized casein impairs antioxidant defense system and induces hepatic and renal injury in mice[J].Food and Chemical Toxicology,2014,64(2):86.
[7]Xiangwu Z,Xiaojun W,Jiao L,et al.Studies on the oxidizing property of sodium hypochlorite solution[J].Chlor-Alkai Ind,2006,(8):28-30.
[8]Liu S X,Hou F F,Guo Z J,et al.Advanced oxidation protein products accelerate atherosclerosis through promoting oxidative stress and inflammation[J].Am Heart Ass Inc,2006:1156-1162.
[9]Boatright W L,Hettiarachchy N S.Effect of lipids on soy protein isolate solubilitu[J].J Am oil Chem Soc,1995,72(12):1439-1444.
[10]Oliver C N,Ahn B W,Moerman E J,et al.Age-related changes in oxidized proteins[J].J Biol Chem,1987,262:5488-5491.
[11]Vanhecke T,Wouters A.Reducing compounds equivocally influence oxidation during digestion of a high-fat beef product,which promotes cytotoxicity in colorectal carcinoma cell lines[J].Journal of Agricultural and Food Chemistry,2016,64:1600-1609.
[12]Estévez M,Ventanas S,Heinonen M.Formation of Strecker aldehydes between protein carbonyls-α-aminoadipic andγ-glutamic semialdehydes-and leucine and isoleucine[J].Food Chem,2011,128:1051-1057.
[13]Papac-Milicevic N,Busch J L,Binde r C J.Malondialdehyde epitopes as targets of immunity and the implications for atherosclerosis[J].Advances in Immunology,2016,(02):001.
[14]吴伶燕,李竹青,吴秋萍.氧化酪蛋白对小鼠组织抗氧化能力及血液肽组成的影响[J].世界科技研究与发展,2011,37(33):489-493.
[15]李婷婷,彭军.次氯酸介导的氧化应激及其病理生理学意义[J].中国药理学与毒理学杂志,2010,24(4):307-310.
[16]吴伟.蛋白质氧化对大豆蛋白结构和凝胶性质的影响[D].无锡:江南大学,2010.
[17]Li ZL,Shi Y,DingY,et al.Dietary oxidized tyrosine(TOP)stimulates TGF-β1-induced extracellular matrix production via the JNK/p38 signaling pathway in rat kidneys[J].Amino Acids,2017,49(2):241-260.
[18]Jacobson E,Olsson O,Nystrom T.Progression and specificity of protein oxidation in the life cycle of Arabidopsis thaliana[J].Bio Chem,2004,279:22204-22208.
[19]Reuter S,Gupta S C,Chaturvedi M M,et al.Oxidative stress,inflammation and cancer:how are they linked[J].Free Radic Biol Med,2010,49:1603-1616.
[20]Estévez M,Luna C.Dietary protein oxidation:A silent threat to human health[J].Critical Reviews in Food Science and Nutrition,2016.