深圳市大鹏新区德国小蠊抗药性及与抗药性相关的几种酶活性关系的研究
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摘要
目的及时了解并掌握深圳市大鹏新区内德国小蠊抗药性的现状,同时探讨德国小蠊抗性产生的生化机理与其体内相关酶活性的关系,为指导德国小蠊综合防治提供理论参考和科学依据。方法采用药膜法测定采自大鹏新区内盛华源餐厅,葵涌市场,葵兴小区,大鹏山庄,较场尾,南澳南天阁海域酒店,大鹏妇幼保健院,沙岗村民宿区8个采样点并混合饲养至F1代的德国小蠊对6种常用杀虫剂的抗性水平;采用分光光度法测定7种酶的活力。结果新区内德国小蠊对氯菊酯、β-氯氰菊酯、溴氰菊酯、残杀威、乙酰甲胺磷和毒死蜱6种杀虫剂的抗性倍数(R/S)分别为5.61、3.10、10.34、2.36、6.90和3.93;对德国小蠊野外和敏感品系体内7种酶(GSTs、AChE、AKP、ACP、SOD、Na-K-ATP、Ca-ATP)活性测定结果显示野外品系均高于敏感品系,经统计学分析,野外品系体内GSTs、AChE和SOD活性与敏感品系相比差异有异统计学意义(P<0.05)。结论深圳市大鹏新区德国小蠊对6种常用杀虫剂均产生了抗药性,其中对溴氰菊酯产生高度抗性,其余均处于中低抗水平,化学防治上建议停用溴氰菊酯,慎用氯菊酯,改用抗性较低的β-氯氰菊酯;德国小蠊体内的GSTs、AChE和SOD与抗药性的形成关系密切,其活性的升高,导致了德国小蠊抗性的产生和发展。
Objective To keep abreast of the current resistance status of Blattella germanica,and to explore the related of the resistance mechanism of Blattella germanica its enzyme activity in Dapeng new district of Shenzhen,so as to provide a scientific basis to use insecticides reasonably.Methods Residual film was used to identify the resistance level of Blattella germanicato to six kinds of insecticides.The samples were collected from 8 points and raised to the F1 generation.The enzyme activity was tested by spectrophotometry.Results The resistance index(R/S) of Blattella germanica topermethrin,beta-cypermethrin,deltamethrin,propoxur,acephate and chlorpyrifos were 5.61,3.10,10.34,2.36,6.90 and 3.93,respectively.The results of the enzymes(GSTs,AChE,AKP,ACP,SOD,Na-K-ATP,Ca-ATP) activity test in the wild strains were higher than sensitive strains,as the GSTs,AchE and SOD activity compared with sensitive strains had a statistically significant difference(P<0.05).Conclusion Among the six used insecticides,the resistance level to deltamethrin is the highest,the rest are in middle and low level in Dapeng new district of Shenzhen.It is recommended to replace the deltamethrin with the beta-cypermethrin in chemical control.Meanwhile,the development of resistance is closely related to the enzyme of GSTs,AChE and SOD in which activity has an increasing trend resulted in the generation and development of Blattella germanica resistance.
Objective To keep abreast of the current resistance status of Blattella germanica,and to explore the related of the resistance mechanism of Blattella germanica its enzyme activity in Dapeng new district of Shenzhen,so as to provide a scientific basis to use insecticides reasonably.Methods Residual film was used to identify the resistance level of Blattella germanicato to six kinds of insecticides.The samples were collected from 8 points and raised to the F1 generation.The enzyme activity was tested by spectrophotometry.Results The resistance index(R/S) of Blattella germanica topermethrin,beta-cypermethrin,deltamethrin,propoxur,acephate and chlorpyrifos were 5.61,3.10,10.34,2.36,6.90 and 3.93,respectively.The results of the enzymes(GSTs,AChE,AKP,ACP,SOD,Na-K-ATP,Ca-ATP) activity test in the wild strains were higher than sensitive strains,as the GSTs,AchE and SOD activity compared with sensitive strains had a statistically significant difference(P<0.05).Conclusion Among the six used insecticides,the resistance level to deltamethrin is the highest,the rest are in middle and low level in Dapeng new district of Shenzhen.It is recommended to replace the deltamethrin with the beta-cypermethrin in chemical control.Meanwhile,the development of resistance is closely related to the enzyme of GSTs,AChE and SOD in which activity has an increasing trend resulted in the generation and development of Blattella germanica resistance.
引文
[1] 张园园,霍新北,刘浩,等.德国小蠊抗药性与生物适合度[J].预防医学论坛,2013,19(10):762-763,766.
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[3] 杨闳智,钟积成,刘坚基,等.2016-2017年深圳市大鹏新区蜚蠊密度监测结果分析[J].现代预防医学,2018,45(5):916-919.
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[5] 苏庆盛,罗林峰,林琳,等.深圳市德国小蠊和家蝇对常用杀虫剂的抗性研究[J].现代预防医学,2014,41(4):698-699,703.
[6] 张彦,张东彦,郭郁,等.北京市丰台区德国小蠊对常用杀虫剂的抗药性调查[J].中国媒介生物学及控制杂志,2013,24(5):439-441.
[7] 吴志凤,王以燕,顾宝根.我国卫生杀虫剂的现状与发展前景[J].农药科学与管理,2011,32(5):16-19.
[8] 孙俊,杨维芳,徐燕.我国蜚蠊及其防治研究概述[J].中国媒介生物学及控制杂志,2009,20(4):275-280.
[9] 朱光峰.德国小蠊抗药性及其抗性衰退情况研究[J].浙江预防医学,2004,16(9):5-6.
[10] Jeong KY,Jeong KJ,Yi MH,et al.Allergenicity of sigma and delta class glutathione S-Transferases from the German cockroach[J].International Archives of Allergy and Immunology,2009,148(1):59-64.
[11] 马义,霍新北,马民,等.高效氯氰菊酯对德国小蠊超氧化物歧化酶诱导作用研究[J].寄生虫与医学昆虫学报,2005,12(1):37-40.
[12] 唐振华.昆虫抗药性的遗传与进化[M].北京:科学技术文献出版社,2000:190-191.
[13] 郭丽,沈孝兵.德国小蠊DDVP抗性与相关酶活性的关系[J].中国公共卫生,2005,48(8):938-939.
[2] 赵霞,张仁利,张韶华,等.德国小蠊抗药性及抗性机理的研究进展[J].热带医学杂志,2009,9(4):455-459.
[3] 杨闳智,钟积成,刘坚基,等.2016-2017年深圳市大鹏新区蜚蠊密度监测结果分析[J].现代预防医学,2018,45(5):916-919.
[4] Lee LC,Lee CY.Insecticide resistance profiles and possible underlying mechanisms in German cockroaches,Blattella germanica (Linnaeus)(Dictyoptera:Blattellidae) from Peninsular Malaysia[J].Medical Entomology and Zoology,2016,55(2):77-93.
[5] 苏庆盛,罗林峰,林琳,等.深圳市德国小蠊和家蝇对常用杀虫剂的抗性研究[J].现代预防医学,2014,41(4):698-699,703.
[6] 张彦,张东彦,郭郁,等.北京市丰台区德国小蠊对常用杀虫剂的抗药性调查[J].中国媒介生物学及控制杂志,2013,24(5):439-441.
[7] 吴志凤,王以燕,顾宝根.我国卫生杀虫剂的现状与发展前景[J].农药科学与管理,2011,32(5):16-19.
[8] 孙俊,杨维芳,徐燕.我国蜚蠊及其防治研究概述[J].中国媒介生物学及控制杂志,2009,20(4):275-280.
[9] 朱光峰.德国小蠊抗药性及其抗性衰退情况研究[J].浙江预防医学,2004,16(9):5-6.
[10] Jeong KY,Jeong KJ,Yi MH,et al.Allergenicity of sigma and delta class glutathione S-Transferases from the German cockroach[J].International Archives of Allergy and Immunology,2009,148(1):59-64.
[11] 马义,霍新北,马民,等.高效氯氰菊酯对德国小蠊超氧化物歧化酶诱导作用研究[J].寄生虫与医学昆虫学报,2005,12(1):37-40.
[12] 唐振华.昆虫抗药性的遗传与进化[M].北京:科学技术文献出版社,2000:190-191.
[13] 郭丽,沈孝兵.德国小蠊DDVP抗性与相关酶活性的关系[J].中国公共卫生,2005,48(8):938-939.