假升麻提取物对大鼠皮肤创伤的治疗作用
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摘要
目的评价假升麻提取物对大鼠皮肤创伤的作用。方法从性状鉴别、DNA分子鉴别两个方面对药材进行种属鉴定;采用打孔法考察假升麻提取物的体外抑菌效果;建立SD大鼠创伤感染模型,大鼠给予假升麻提取物治疗16 d后,以创面形态、创面愈合率、组织病理学、免疫组化为指标评价其治疗效果。结果鉴定药材基原植物为蔷薇科(Rosaceae)假升麻属(Aruncus)植物假升麻(Aruncus sylvester Kostel.);假升麻提取物对金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌均具有一定的体外抑菌作用;动物创伤感染实验发现,大鼠创面感染治疗第12天,给药组伤口结痂完全脱落,伤口边缘处可见粉嫩的新生皮肤,模型组伤口表面干燥,给药组伤口表面脓状物明显少于模型组。治疗第16天,给药组创伤基本愈合,创面愈合率>95%,模型组创面愈合率仍未达愈合标准。组织病理分析表明给药组角质层、表皮层、真皮层规则有序排列,模型组存在严重的表皮增生现象。给药组组织中有明显的血管内皮生长因子的表达,表明有大量的新生血管生成。结论假升麻提取物对细菌感染性皮肤创伤具有一定的促伤口愈合能力,有可能用于治疗皮肤创伤。
Objective To evaluate the effect of Aruncus sylvester extract on the open wound of skin in rats. Methods The plant species of medicinal materials was identified by the morphological investigation and DNA molecular identification. The in vitro antibacterial activity was assayed by the agar hole diffusion test. The traumatic infection model of SD rats was established and divided into model group and drug treatment group. After 16 days of the drug-treatment,the therapeutic effects were evaluated with the wound morphology,wound healing rate,histopathology and immunohistochemical analysis. Results The original plant of the medicinal materials was identified as Aruncus sylvester Kostel.(Rosaceae). The extract of A. sylvester showed a certain inhibitory effect on Staphylococcus aureus,Pseudomonas aeruginosa and Escherichia coli. On the 12 th day of treatment in the animal test,the scab was completely removed from the wound and fresh pink skin was seen on the edge of the wound in the drug treatment group. The wound surface of rats in the model group was dry. The pus on the wound surface in the treatment group was significantly less than those in the model group.On the 16 th day of treatment,the wound healing rate was over 95% and the wound was almost healed up in the treatment group,while the wound healing rate did not reach the healing standard in the model group. Histopathological analysis showed that the cuticle,epidermis and dermis were orderly arranged in the treatment group,while serious epidermal hyperplasia was seen in the model group.The expression of vascular endothelial growth factor was obviously increased in the wound tissues in the treatment group,indicating plentiful angiogenesis. Conclusion The A. sylvester extract showed a potential ability to promote wound healing of infectious skin and was likely used to treat open skin wounds.
Objective To evaluate the effect of Aruncus sylvester extract on the open wound of skin in rats. Methods The plant species of medicinal materials was identified by the morphological investigation and DNA molecular identification. The in vitro antibacterial activity was assayed by the agar hole diffusion test. The traumatic infection model of SD rats was established and divided into model group and drug treatment group. After 16 days of the drug-treatment,the therapeutic effects were evaluated with the wound morphology,wound healing rate,histopathology and immunohistochemical analysis. Results The original plant of the medicinal materials was identified as Aruncus sylvester Kostel.(Rosaceae). The extract of A. sylvester showed a certain inhibitory effect on Staphylococcus aureus,Pseudomonas aeruginosa and Escherichia coli. On the 12 th day of treatment in the animal test,the scab was completely removed from the wound and fresh pink skin was seen on the edge of the wound in the drug treatment group. The wound surface of rats in the model group was dry. The pus on the wound surface in the treatment group was significantly less than those in the model group.On the 16 th day of treatment,the wound healing rate was over 95% and the wound was almost healed up in the treatment group,while the wound healing rate did not reach the healing standard in the model group. Histopathological analysis showed that the cuticle,epidermis and dermis were orderly arranged in the treatment group,while serious epidermal hyperplasia was seen in the model group.The expression of vascular endothelial growth factor was obviously increased in the wound tissues in the treatment group,indicating plentiful angiogenesis. Conclusion The A. sylvester extract showed a potential ability to promote wound healing of infectious skin and was likely used to treat open skin wounds.
引文
[1]Barberán J,Fari?as MC.Daptomycin in complicated skin and soft tissue infections[J].En Ferm Infecc Microbiol Clin,2012,30(1):33-37.
[2]李晓康,王舒,俞洋.皮肤创伤修复研究进展[J].中国中西医结合皮肤性病学杂志,2016,15(1):62-65.
[3]Meng Q,Su YF,Yan SL,et al.Two new phenylpropanoid glycosides from the roots of Aruncus sylvester[J].Asian Nat Prod Res,2014,16(2):158-162.
[4]郭增军.陕西七药[M].西安:陕西科学技术出版社,2003.
[5]Sokikiwsja WA,Nowak R.Phenolic compounds from Aruncus sylvester Kost.[J].Herb Polon,2000,46(4):255-260.
[6]Su YJ,Do YJ,Young HK,et al.Monoterpenoids from the aerial parts of Aruncus dioicus var.kamtschaticus,and their antioxidant and cytotoxic activities[J].Bioorg Med Chem Lett,2011,21(11):3252-3256.
[7]原嫄.假升麻乙醇提取物乙酸乙酯萃取部分、石油醚萃取部分的化学成分研究[D].天津:天津大学,2013.
[8]中国科学院《中国植物志》编辑委员会.中国植物志[M].北京:科学出版社,2004.
[9]蔡恩博,韩佳宏,蕾李.假升麻根乙醇提取物抑菌活性、抗氧化作用及其根中微量元素测定[J].食品科学,2010,31(11):97-101.
[10]罗美蓉,王正伟,安志星.人血管内皮生长因子研究进展[J].中国老年学,2012,32(17):3835-3837.
[11]Moens S,Goveia J,Stapor PC,et al.The multifaceted activity of VEGF in angiogenesis-Implications for therapy responses[J].Cytokine Growth Factor Rev,2014,25(4):473-482.
[12]王韧,候立军.VEGF和内皮抑素对血管生成作用的研究进展[J].重庆医学,2011,40(25):2582-2585.
[13]王艳杰.愈合散对大鼠皮肤创面组织愈合及bFGF的影响研究[D],成都:成都体育学院,2013.20181025 20190108
[2]李晓康,王舒,俞洋.皮肤创伤修复研究进展[J].中国中西医结合皮肤性病学杂志,2016,15(1):62-65.
[3]Meng Q,Su YF,Yan SL,et al.Two new phenylpropanoid glycosides from the roots of Aruncus sylvester[J].Asian Nat Prod Res,2014,16(2):158-162.
[4]郭增军.陕西七药[M].西安:陕西科学技术出版社,2003.
[5]Sokikiwsja WA,Nowak R.Phenolic compounds from Aruncus sylvester Kost.[J].Herb Polon,2000,46(4):255-260.
[6]Su YJ,Do YJ,Young HK,et al.Monoterpenoids from the aerial parts of Aruncus dioicus var.kamtschaticus,and their antioxidant and cytotoxic activities[J].Bioorg Med Chem Lett,2011,21(11):3252-3256.
[7]原嫄.假升麻乙醇提取物乙酸乙酯萃取部分、石油醚萃取部分的化学成分研究[D].天津:天津大学,2013.
[8]中国科学院《中国植物志》编辑委员会.中国植物志[M].北京:科学出版社,2004.
[9]蔡恩博,韩佳宏,蕾李.假升麻根乙醇提取物抑菌活性、抗氧化作用及其根中微量元素测定[J].食品科学,2010,31(11):97-101.
[10]罗美蓉,王正伟,安志星.人血管内皮生长因子研究进展[J].中国老年学,2012,32(17):3835-3837.
[11]Moens S,Goveia J,Stapor PC,et al.The multifaceted activity of VEGF in angiogenesis-Implications for therapy responses[J].Cytokine Growth Factor Rev,2014,25(4):473-482.
[12]王韧,候立军.VEGF和内皮抑素对血管生成作用的研究进展[J].重庆医学,2011,40(25):2582-2585.
[13]王艳杰.愈合散对大鼠皮肤创面组织愈合及bFGF的影响研究[D],成都:成都体育学院,2013.20181025 20190108