GC-MS法分析得荣县光核桃仁油中脂溶性成分
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摘要
目的采用GC-MS法分析得荣县光核桃Prunus mira Koehne仁油中脂溶性成分。方法热榨法榨取光核桃仁油,GC-MS法分离、鉴定,并测定各个化合物相对含有量,对结果进行聚类分析和主成分分析。结果夺松村的光核桃仁油中有20个化合物,β-谷甾醇、反式角鲨烯和油酸羟丙基酯含有量较高,1个特有化合物(4-甲基壬烷);日堆村的有22个化合物,β-谷甾醇、油酸和γ-生育酚含有量较高,1个特有化合物(1,2,4-三甲基环己烷);绒贡村的有24个化合物,油酸、β-谷甾醇和反式角鲨烯含有量较高;张仁村的有22个化合物,以β-谷甾醇、油酸和γ-生育酚含有量较高,2个特有化合物(反式-2,4-癸二烯醛和二十八醛)。10批样品分为6类。夺松村第一主成分的累积贡献率81.86%。结论得荣县光核桃仁油有35个化合物,以油酸、β-谷甾醇、反式角鲨烯、γ-生育酚和维生素E为主,特有化合物4个。
AIM To analyze fat-soluble components of nuts oil from Prunus mira Koehne in Derong County.METHODS The nuts oil from P. mira was extracted by hot pressing method and then was isolated and identified by GC-MS,together with relative content determination of various compounds. The results were analyzed by cluster analysis and principal component analysis. RESULTS Twenty compounds were isolated from nuts oil in Duosong Village,with higher contents of β-sitosterol,trans-squalene,oleic acid hydroxypropyl ester,and one endemic compound( 4-methyl nonane). Twenty-two compounds were isolated from nuts oil in Ridui Village,with higher contents of β-sitosterol,oleic acid,γ-tocopherol,and one endemic compound( cyclohexane,1,2,4-trimethyl-).Twenty-four compounds were isolated from nuts oil in Ronggong Village,with higher contents of oleic acid,β-sitosterol and trans-squalene. Twenty-two compounds were isolated from nuts oil in Zhangren Village,with higher contents of β-sitosterol,oleic acid,γ-tocopherol,and two endemic compounds( trans-2,4-decadienal and octacosanal). Ten batches of samples were divided into six types. The cumulative contribution rate of the first principal component was 81. 86% in Duosong Village. CONCLUSION Thirty-five compounds in nuts oil from P. mira in Derong County mainly contain oleic acid,β-sitosterol,trans-squalene,γ-tocopherol and vitamin E,with four endemic compounds.
AIM To analyze fat-soluble components of nuts oil from Prunus mira Koehne in Derong County.METHODS The nuts oil from P. mira was extracted by hot pressing method and then was isolated and identified by GC-MS,together with relative content determination of various compounds. The results were analyzed by cluster analysis and principal component analysis. RESULTS Twenty compounds were isolated from nuts oil in Duosong Village,with higher contents of β-sitosterol,trans-squalene,oleic acid hydroxypropyl ester,and one endemic compound( 4-methyl nonane). Twenty-two compounds were isolated from nuts oil in Ridui Village,with higher contents of β-sitosterol,oleic acid,γ-tocopherol,and one endemic compound( cyclohexane,1,2,4-trimethyl-).Twenty-four compounds were isolated from nuts oil in Ronggong Village,with higher contents of oleic acid,β-sitosterol and trans-squalene. Twenty-two compounds were isolated from nuts oil in Zhangren Village,with higher contents of β-sitosterol,oleic acid,γ-tocopherol,and two endemic compounds( trans-2,4-decadienal and octacosanal). Ten batches of samples were divided into six types. The cumulative contribution rate of the first principal component was 81. 86% in Duosong Village. CONCLUSION Thirty-five compounds in nuts oil from P. mira in Derong County mainly contain oleic acid,β-sitosterol,trans-squalene,γ-tocopherol and vitamin E,with four endemic compounds.
引文
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[3]帝玛尔.丹增彭措.晶珠本草[M],1版.上海:上海科学技术出版社,1986:67.
[4]中国科学院西北高原生物研究所.藏药志[M],1版.青海:青海人民出版社,1991:20-22.
[5]国家中医药管理局《中华本草》编委会.中华本草(藏药卷)[M].上海:上海科学技术出版社,2002:247-248.
[6]颜永刚.桃仁质量研究[D].成都:成都中医药大学,2008.
[7]颜永刚,邓翀,刘静,等.桃仁与山桃仁石油醚部位气相色谱-质谱联用分析比较[J].时珍国医国药,2011,22(8):1812-1814.
[8]周建涛,钟永模,王天云,等.川西南光核桃类型及桃的起源[C]//中国园艺学会首届青年学术讨论会论文集.北京:中国园艺学会青年学术讨论会,1994.
[9]张忠泉,邢煜君,胡国强,等.β-谷甾醇诱导人肝癌Hep G2细胞凋亡机制研究[J].中国中药杂志,2011,36(15):2145-2148.
[10]李雪玲,凌玮玮.桃仁中苯甲醛的提取及其抑菌作用研究[J].生物学杂志,2010,27(3):31-33.
[11]梁颖,裴瑾,周明眉,等.GC-MS分析不同品种桃仁的脂溶性成分[J].华西药学杂志,2012,27(2):174-176.
[12]郭婵娟,赵忠,毛巧芝,等.热榨苦杏仁油脂及精油提取最佳条件的选择[J].中南林业科技大学学报,2008,28(5):102-107.
[13]卢斌.黄土高原不同产地苦杏仁和精油含有量差异的原因[D].杨凌:西北农林科技大学,2010.
[14]申烨华,张萍,孔祥虹,等.扁桃油中脂肪酸组成的GC-MS法分析[J].分析试验室,2005,24(9):37-39.
[15]梁颖.红花一桃仁有效组分及制剂的质量评价研究[D].成都:成都中医药大学,2011.