菜豆愈伤组织的诱导效果及其凝集素水平分析
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摘要
以‘Bean Saint esprit a oeil rouge’‘Bean White rice’‘97-5架豆’为材料,以菜豆组培苗的子叶、茎、叶作为外植体,分别在添加不同浓度激素(2,4-D、6-BA、KT)配比的MS培养基中进行培养,观察其愈伤组织诱导情况,并选取长势较好的愈伤组织进行凝集素水平测定。结果表明:3个菜豆品种的子叶、茎、叶愈伤组织的适宜培养基均为MS+3 mg/L 2,4-D+0. 5 mg/L 6-BA+0. 5 mg/L KT。‘Bean Saint esprit a oeil rouge’的子叶与叶愈伤组织中菜豆凝集素的凝集活力最高,为0. 27 HU。因此,使用‘Bean Saint esprit a oeil rouge’的子叶或叶为外植体,在MS+3 mg/L 2,4-D+0. 5 mg/L 6-BA+0. 5 mg/L KT中进行培养可提取较多的菜豆凝集素,为进一步研究基于菜豆凝集素的生物农药提供了试验基础与数据支持。
The ′Bean Saint esprit a oeil rouge′ ′Bean White rice′ ′97-5 racks′ of common bean cultivars were chosen as raw materials. After seed germination,the cotyledons,stems and leaves of the beans were chosen as explants,respectively,and to culture them in MS medium with different concentrations of hormone(2,4-D,6-BA,KT)to observe the induction of callus and determine lectin level in callus. The results showed that the suitable medium of cotyledon,stem and leaf callus in the three common bean cultivars were MS + 3 mg/L 2,4-D + 0. 5 mg/L 6-BA + 0. 5 mg/L KT.The agglutination activity of plant lectin in the cotyledons and leaves callus of ′Bean Saint esprit a oeil rouge′ was the highest,it was 0. 27 HU. In conclusion,the cotyledons or leaves of ′Bean Saint esprit a oeil rouge′ were selected in medium of MS + 3 mg/L 2,4-D + 0. 5 mg/L 6-BA + 0. 5 mg/L KT which could produce the largest amount of lectin,it will provide the experimental basis and data support for further research of artificial production of bean lectin.
The ′Bean Saint esprit a oeil rouge′ ′Bean White rice′ ′97-5 racks′ of common bean cultivars were chosen as raw materials. After seed germination,the cotyledons,stems and leaves of the beans were chosen as explants,respectively,and to culture them in MS medium with different concentrations of hormone(2,4-D,6-BA,KT)to observe the induction of callus and determine lectin level in callus. The results showed that the suitable medium of cotyledon,stem and leaf callus in the three common bean cultivars were MS + 3 mg/L 2,4-D + 0. 5 mg/L 6-BA + 0. 5 mg/L KT.The agglutination activity of plant lectin in the cotyledons and leaves callus of ′Bean Saint esprit a oeil rouge′ was the highest,it was 0. 27 HU. In conclusion,the cotyledons or leaves of ′Bean Saint esprit a oeil rouge′ were selected in medium of MS + 3 mg/L 2,4-D + 0. 5 mg/L 6-BA + 0. 5 mg/L KT which could produce the largest amount of lectin,it will provide the experimental basis and data support for further research of artificial production of bean lectin.
引文
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[13]尚蕊,吴华,郭瑞,等.不同菜豆品种4种抗营养因子水平差异分析[J].园艺学报,2015,42(11):2163-2173.
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[15]任三国,陈高,郭瑞,等.菜豆种质资源的植物凝集素含量测定与评价[J].长江蔬菜,2017(16):36-38.
[2]李斌,陈树良,秦超,等.普通菜豆种质资源研究现状及进展(英文)[J].农业科学与技术(英文版),2016,17(11):2572-2577.
[3] PORCH T G,CICHY K,WANG W J,et al. Nutritional composition and cooking characteristics of tepary bean(Phaseolus acutifolius Gray)in comparison with common bean(Phaseolus vulgaris L.)[J]. Genetic Resources and Crop Evolution,2017,64(5):935-953.
[4]张小霞,曹素梅,梁振普,等.植物凝集素在抗刺吸式害虫转基因工程中的应用[J].植物保护,2010,36(4):23-28.
[5]邓青,陈巨莲,程登发,等.植物凝集素及其抗蚜作用研究进展[J].应用昆虫学报,2013,50(1):268-275.
[6]唐克轩,沈乾,付雪晴,等.植物次生代谢产物生物反应器研究进展[J].中国农业科技导报,2014,16(1):7-15.
[7]韩启厚,古瑜,于海龙.菜豆组织培养及遗传转化研究进展[J].中国农学通报,2013,29(10):97-101.
[8]朱广廉.植物组织培养中灭菌和无菌操作的几个问题[J].植物生理学通讯,1995(5):373-375.
[9]刘庆昌,吴国良.植物细胞组织培养[M].北京:中国农业大学出版社,2010:1,31.
[10]王超.豆类凝集素的提取分离及纯化[D].哈尔滨:哈尔滨工业大学,2013.
[11]杨明亮,王继安.大豆凝集素含量测定及聚类分析[J].大豆科技,2009(5):20-23.
[12]朱有光,奚惕.菜豆的组织培养和植株再生[J].植物生理学通讯,1988(2):60.
[13]尚蕊,吴华,郭瑞,等.不同菜豆品种4种抗营养因子水平差异分析[J].园艺学报,2015,42(11):2163-2173.
[14]包英华,白音,王羽梅,等.豇豆再生体系的建立[J].广东农业科学,2006(4):31-33.
[15]任三国,陈高,郭瑞,等.菜豆种质资源的植物凝集素含量测定与评价[J].长江蔬菜,2017(16):36-38.