姜黄素与β-葡聚糖的结合研究
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摘要
通过紫外可见吸收光谱法探究了吐温80对姜黄素和姜黄素/β-葡聚糖复合物溶解性和稳定性的影响,同时通过激光粒度仪确定了复合物形成的超分子聚集体及其粒径变化,并利用X-射线衍射和红外光谱研究了姜黄素与β-葡聚糖结合前后结构的变化.结果表明:姜黄素在吐温80乙醇溶液中比在乙醇溶液的溶解性和稳定性都好,通过X-射线衍射和红外光谱从一定程度上证实了姜黄素与燕麦β-葡聚糖只是物理结合,通过动态光散射粒径测定吐温80可以促进姜黄素和β-葡聚糖的分散.
The UV-Vis absorption spectroscopy method is used to study the dissolution of curcumin and curcumin/β-glucan complex by Tween 80. The effects of properties and stability, and the supramolecular aggregates formed by the complexes and their particle size changes were determined by laser particle size analyzer. Curcumin and β-glucan were studied by X-ray diffraction and infrared spectroscopy. After combining changes in the structure before and after, the results showed that the solubility and stability of curcumin in Tween 80 ethanol solution was better than that in ethanol solution. There was no change in the effect of spectrum both in absorbance and shift of the wavelength, respectively.X-ray diffraction and infrared spectroscopy, to some extent, confirmed that curcumin and oat β-glucan are only physically bound. Particle size measurement showed that Tween 80 promoted the dispersion of curcumin and β-glucan.
The UV-Vis absorption spectroscopy method is used to study the dissolution of curcumin and curcumin/β-glucan complex by Tween 80. The effects of properties and stability, and the supramolecular aggregates formed by the complexes and their particle size changes were determined by laser particle size analyzer. Curcumin and β-glucan were studied by X-ray diffraction and infrared spectroscopy. After combining changes in the structure before and after, the results showed that the solubility and stability of curcumin in Tween 80 ethanol solution was better than that in ethanol solution. There was no change in the effect of spectrum both in absorbance and shift of the wavelength, respectively.X-ray diffraction and infrared spectroscopy, to some extent, confirmed that curcumin and oat β-glucan are only physically bound. Particle size measurement showed that Tween 80 promoted the dispersion of curcumin and β-glucan.
引文
[1]申瑞玲.燕麦β-葡聚糖的提取纯化及功能特性研究[D].无锡:江南大学,2005.
[2]SKENDI A,BILIADERIS C,LAZARIDOU A,et al.Structure and rheological properties of water soluble β-glucans from oat cultivars of Avena sativa and Avena bysantina[J].Journal of Cereal Science,2003,38(1):15-31.
[3]PAPAGEORGIOU M ,LAKHDARA N ,LAZARIDOU A ,et al.Water extractable (1→3,1→4)-β-d-glucans from barley and oats:an intervarietal study on their structural features and rheological behaviour[J].Journal of Cereal Science,2005,42(2):213-224.
[4]KIRKPATRICK U J ,ADAMS R A ,LARDI A ,et al.Rheological properties and function of blood cells in stored bank blood and salvaged blood[J].British Journal of Haematology,1998,101(2):364–368.
[5]KAHLON T S,CHOW F I,KNUCKLES B E,et al.Cholesterol-lowering effects in hamsters of beta-glucan-enriched barley fraction,dehulled whole barley,rice bran,and oat bran and their combinations[J].Cereal Chemistry,1993,70(4):435-440.
[6]JENKINS D,WOLEVER T,TAYLOR R H,et al.Glycemic index of foods:a physiological basis for carbohydrate exchange[J].The American Journal of Clinical Nutrition,1981,34(3):362-366.
[7]周坚,肖安红.功能性膳食纤维食品[M].北京:化学工业出版社,2005.
[8]BELL S,GOLDMAN V M,BISTRIAN B R,et al.Effect of β-glucan from oats and yeast on serum lipids[J].Critical Reviews in Food Science and Nutrition,1999,39(2):189-202.
[9]方瑜,向柏,潘振,等.姜黄素缓释滴丸的制备及体外释放度考察[J].中药材,2010,33(1):111-114.
[10]刘敏,赵远航,王丽娟,等.载带姜黄素的卵磷脂/果胶锌凝胶球的性能[J].天津科技大学学报,2014(4):44-47.
[11]夏书芹,许时婴.吐温80增溶-紫外分光光度法测定辅酶Q10脂质体的载量及包封率[J].食品与发酵工业,2005,31(10):131-135.
[12]ROHMAN A,SUDJADI,DEVI,et al.Analysis of curcumin in curcuma longa and curcuma xanthorriza using FTIR spectroscopy and chemometrics[J].Research Journal of Medicinal Plant,2015(3):155-160.
[13]邵君飞,姜志峰,孙骏,等.姜黄素载药纳米微球的制备、表征及性质[J].江苏医药,2010,36(20):2435-2437.
[14]KATSUYAMA Y,KITA T,FUNA N,et al.Curcuminoid biosynthesis by two type Ⅲ polyketide synthases in the herb lurcuma longa[J].Journal of Biological Chemistry,2009,284(17):11160-11170.
[15]张维杰.糖复合物生化研究技术[M].杭州:浙江大学出版社,1999:193-198.
[16]黄秀旺,许建华,温彩霞.姜黄素-聚维酮固体分散体的制备及溶出度的测定[J].中国医院药学杂志,2008,28(21):1819-1822.
[17]HRMOVA M,GEOFFREY B FINCHER.Structure-function relationships of β-D-glucan endo-and exohydrolases from higher plants[J].Plant Molecular Biology,2001,47(1/2):73-91.
[2]SKENDI A,BILIADERIS C,LAZARIDOU A,et al.Structure and rheological properties of water soluble β-glucans from oat cultivars of Avena sativa and Avena bysantina[J].Journal of Cereal Science,2003,38(1):15-31.
[3]PAPAGEORGIOU M ,LAKHDARA N ,LAZARIDOU A ,et al.Water extractable (1→3,1→4)-β-d-glucans from barley and oats:an intervarietal study on their structural features and rheological behaviour[J].Journal of Cereal Science,2005,42(2):213-224.
[4]KIRKPATRICK U J ,ADAMS R A ,LARDI A ,et al.Rheological properties and function of blood cells in stored bank blood and salvaged blood[J].British Journal of Haematology,1998,101(2):364–368.
[5]KAHLON T S,CHOW F I,KNUCKLES B E,et al.Cholesterol-lowering effects in hamsters of beta-glucan-enriched barley fraction,dehulled whole barley,rice bran,and oat bran and their combinations[J].Cereal Chemistry,1993,70(4):435-440.
[6]JENKINS D,WOLEVER T,TAYLOR R H,et al.Glycemic index of foods:a physiological basis for carbohydrate exchange[J].The American Journal of Clinical Nutrition,1981,34(3):362-366.
[7]周坚,肖安红.功能性膳食纤维食品[M].北京:化学工业出版社,2005.
[8]BELL S,GOLDMAN V M,BISTRIAN B R,et al.Effect of β-glucan from oats and yeast on serum lipids[J].Critical Reviews in Food Science and Nutrition,1999,39(2):189-202.
[9]方瑜,向柏,潘振,等.姜黄素缓释滴丸的制备及体外释放度考察[J].中药材,2010,33(1):111-114.
[10]刘敏,赵远航,王丽娟,等.载带姜黄素的卵磷脂/果胶锌凝胶球的性能[J].天津科技大学学报,2014(4):44-47.
[11]夏书芹,许时婴.吐温80增溶-紫外分光光度法测定辅酶Q10脂质体的载量及包封率[J].食品与发酵工业,2005,31(10):131-135.
[12]ROHMAN A,SUDJADI,DEVI,et al.Analysis of curcumin in curcuma longa and curcuma xanthorriza using FTIR spectroscopy and chemometrics[J].Research Journal of Medicinal Plant,2015(3):155-160.
[13]邵君飞,姜志峰,孙骏,等.姜黄素载药纳米微球的制备、表征及性质[J].江苏医药,2010,36(20):2435-2437.
[14]KATSUYAMA Y,KITA T,FUNA N,et al.Curcuminoid biosynthesis by two type Ⅲ polyketide synthases in the herb lurcuma longa[J].Journal of Biological Chemistry,2009,284(17):11160-11170.
[15]张维杰.糖复合物生化研究技术[M].杭州:浙江大学出版社,1999:193-198.
[16]黄秀旺,许建华,温彩霞.姜黄素-聚维酮固体分散体的制备及溶出度的测定[J].中国医院药学杂志,2008,28(21):1819-1822.
[17]HRMOVA M,GEOFFREY B FINCHER.Structure-function relationships of β-D-glucan endo-and exohydrolases from higher plants[J].Plant Molecular Biology,2001,47(1/2):73-91.