鹿气管硫酸软骨素的提取分离与抗氧化活性研究
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摘要
分别采用稀碱-酶解法和稀碱-浓盐法提取鹿气管中的硫酸软骨素,通过比较提取率、提取时间等对2种方法进行考察,最终选取提取率高、耗时短的稀碱-浓盐法提取其中的硫酸软骨素。采用高效液相色谱法对鹿气管中硫酸软骨素含量进行测定,并探讨鹿气管硫酸软骨素及硫酸软骨素寡糖的抗氧化活性。结果发现,稀碱-浓盐法提取鹿气管中硫酸软骨素的得率为21.88%;鹿气管中总硫酸软骨素含量为35.31 g/kg,硫酸软骨素A、B和C含量分别为19.54、2.06 g/kg和13.71 g/kg;鹿气管硫酸软骨素及其寡糖均具有一定的抗氧化活性,且硫酸软骨素寡糖的抗氧化活性显著高于硫酸软骨素。
Dilute alkali-enzymatic hydrolysis method and dilute alkali-concentrated salt method were used to extract chondroitin sulfate from deer trachea by comparing extraction rate and extraction time. Finally, dilute alkali-concentrated salt method with high extraction rate and short time was chosen to extract chondroitin sulfate from that. High performance liquid chromatography(HPLC) was used to determined chondroitin sulfate content in deer trachea, and antioxidant activity of chondroitin sulfate and chondroitin sulfate oligosaccharides from deer tracheal was investigated. The results showed that yield of chondroitin sulfate from deer trachea was 21.88% by dilute alkali-concentrated salt extraction, total chondroitin sulfate content in deer trachea was 35.31 g/kg, and chondroitin sulfate A, B, and C contents were 19.54, 2.06 and 13.71 g/kg, respectively. Chondroitin sulfate and chondroitin sulfate oligosaccharides from deer trachea both have certain antioxidant activity, and antioxidant activity of chondroitin sulfate oligosaccharide was significantly higher than that of chondroitin sulfate from deer tracheal.
Dilute alkali-enzymatic hydrolysis method and dilute alkali-concentrated salt method were used to extract chondroitin sulfate from deer trachea by comparing extraction rate and extraction time. Finally, dilute alkali-concentrated salt method with high extraction rate and short time was chosen to extract chondroitin sulfate from that. High performance liquid chromatography(HPLC) was used to determined chondroitin sulfate content in deer trachea, and antioxidant activity of chondroitin sulfate and chondroitin sulfate oligosaccharides from deer tracheal was investigated. The results showed that yield of chondroitin sulfate from deer trachea was 21.88% by dilute alkali-concentrated salt extraction, total chondroitin sulfate content in deer trachea was 35.31 g/kg, and chondroitin sulfate A, B, and C contents were 19.54, 2.06 and 13.71 g/kg, respectively. Chondroitin sulfate and chondroitin sulfate oligosaccharides from deer trachea both have certain antioxidant activity, and antioxidant activity of chondroitin sulfate oligosaccharide was significantly higher than that of chondroitin sulfate from deer tracheal.
引文
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[3] Campo R D, Romano J E. Changes in cartilage proteoglycans associated with calcification[J]. Calcified Tissue International, 1986,39(3):175-184
[4] Pumphrey C Y, Theus A M, Li S, et al. Neoglycans,carbodiimide-modified glycosaminoglycans:a new class of anticancer agents that inhibit cancer cell proliferation and induce apoptosis[J]. Cancer Research,2002,62(13):3722
[5] Zhou Q L,Guo Y J,Wang L J, et al. Velvet antler polypeptides promoted proliferation of chondrocytes and osteoblast precursors and fracture healing[J]. Acta Pharmaologica Sinica, 1999,20(3):279-282
[6]宫瑞泽,王燕华,孙印石.不同加工方式及不同部位鹿茸中硫酸软骨素的含量分析[J].中国中药杂志,2018,(3):557-562
[7]徐传屯,关瑞章,郑江.硫酸软骨素测定方法的研究进展[J].集美大学学报(自然科学版),2008,13(1):52-56
[8]中华人民共和国药典(2015年版,第2部)[S].北京:中国医药科技出版社,2015,1340
[9]李珊珊,祝贺,祁玉丽,等.人参果多糖的分离纯化及体外抗氧化活性研究[J].食品工业科技,2018,(4):73-76
[10]吕爽,田呈瑞,王虎,等.不同薄荷多酚、总黄酮及体外抗氧化性比较[J].食品工业科技,2011,(8):160-163
[11]张静,张京良,朱常亮,等.酶法制备硫酸软骨素寡糖及其抗氧化活性[J].食品工业科技,2017,13:48-52
[12]王玉田.动物性副产品加工利用[M].北京:化学工业出版社,2009,152
[13]刘莎,沈建如,于荣敏.不同动物来源中硫酸软骨素的提取及降压活性初探[J].中药材,2010,33(2):180-183
[14]熊双丽,金征宇.不同分子量硫酸软骨素的制备和抗氧化活性探讨[J].中成药,2006,28(9):1343-1346
[15]张莲,王金鹏,孔子青,等.硫酸软骨素的降解及其降解产物抗氧化活性的测定[J].食品工业科技,2011,(12):180-182
[16]谢捷,汤君敏,朱兴一,等.不同硫酸酯基含量硫酸软骨素的体外抗氧化活性[J].浙江工业大学学报,2010,38(6):608-610
[17]王俊,曾凡新,程薇,等.不同降解方式对硫酸软骨素寡糖抗氧化活性的影响[J].湖北农业科学,2015,54(22):5707-5710
[18] Deepa S S, Kalayanamitra K, Ito Y, et al. Novel sulfated octa-and decasaccharides from squid cartilage chondroitin sulfate E:sequencing and application for determination of the epitope structure of the monoclonal antibody MO-225[J]. Biochemistry,2007,46(9):2453-2465
[19] Abad L V,Relleve L S,Racadio C D,et al. Antioxidant activity potential of gamma irradiated carrageenan[J].Applied Radiation&Isotopes,2013,79(3):73-79
[20] Campo G M,Avenoso A,Campo S,et al. The antioxidant and antifibrogenic effects of the glycosaminoglycans hyaluronic acid and chondroitin-4-sulphate in a subchronic rat model of carbon tetrachloride-induced liver fibrogenesis[J]. Chemico-biological interactions,2004,148(3):125-138