西洋参果多糖的纯化及DPPH自由基清除活性研究
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摘要
通过水提醇沉法得到西洋参果中总多糖(WQBP),再通过离子交换层析分离纯化,得到中性糖WQBP-N和酸性糖WQBP-A。利用高效液相色谱法对单糖组成进行分析,并检测各级分的1,1-二苯基-2-二硝基苯肼(DPPH)自由基清除活性。结果表明,WQBP主要由鼠李糖(Rhamnose,Rha)、半乳糖醛酸(Galacturonic acid,GalA)、半乳糖(Galactose,Gal)、阿拉伯糖(Arabinose,Ara)和葡萄糖(Glucose,Glc)组成;WQBP-N主要由Gal、Ara、Glc和甘露糖组成,比例为63.9∶19.3∶15.2∶1.5;WQBP-A主要由Rha、GalA、Gal、Ara和Glc构成,比例为13.1∶13.2∶46.4∶19.0∶5.8。WQBP对DPPH自由基清除活性优于其子级分,并且清除率随着浓度的升高而增加。研究结果能够为西洋参果多糖的开发利用提供一定的数据基础。
Extracted and purified polysaccharide fractions from Panax quinquefolius berry,and DPPH radicals scavenging activity was carried on.Polysaccharide fractions(WQBP) from P.quinquefolius berry was dealed with water-extraction, ethanol-precipetation and ion-exchange chromatography,then one neutral(WQBP-N) and one acidic fraction(WQBP-A) were obtained.The monosaccharide composition,molecular weight distribution and DPPH free radicals scavenging activity were analyzed.WQBP is mainly composed of rhamnose, galacturonic acid, galactose, arabinose and glucose. WQBP-N is composed of galactose, arabinose, glucose and mannose with a ratio of63.9∶19.3∶15.2∶1.5; WQBP-A is mainly composed by rhamnose, galacturonic acid,galactose, arabinose and glucose with a ratio of13.1∶13.2∶46.4∶19.0∶5.8.WQBP has more significant DPPH free radicals scavenging activity than neutral and acidic fraction, which has a concentration dependant tendency.WQBP has potential antioxidant activity in vitro, and the results above could provide theory basis for the further application and developments of the P.quinquefolius berry polysaccharide.
Extracted and purified polysaccharide fractions from Panax quinquefolius berry,and DPPH radicals scavenging activity was carried on.Polysaccharide fractions(WQBP) from P.quinquefolius berry was dealed with water-extraction, ethanol-precipetation and ion-exchange chromatography,then one neutral(WQBP-N) and one acidic fraction(WQBP-A) were obtained.The monosaccharide composition,molecular weight distribution and DPPH free radicals scavenging activity were analyzed.WQBP is mainly composed of rhamnose, galacturonic acid, galactose, arabinose and glucose. WQBP-N is composed of galactose, arabinose, glucose and mannose with a ratio of63.9∶19.3∶15.2∶1.5; WQBP-A is mainly composed by rhamnose, galacturonic acid,galactose, arabinose and glucose with a ratio of13.1∶13.2∶46.4∶19.0∶5.8.WQBP has more significant DPPH free radicals scavenging activity than neutral and acidic fraction, which has a concentration dependant tendency.WQBP has potential antioxidant activity in vitro, and the results above could provide theory basis for the further application and developments of the P.quinquefolius berry polysaccharide.
引文
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[12] Liu J, Willfor S, Xu CL. A review of bioactive plant pol-ysaccharides:biological activities, functionalization, and bio-medical applications[J]. Bioactive Carbohydrate Dietary Fib-re, 2015,(5):31-61.
[2] Xie JT,Wu JA,Mehendale S,et al.Anti-hyperglycemic effect ofthe polysaccharides fraction from American ginseng berry ex-tract in ob/ob mice[J]. Phytomedicine, 2004, 11:182-187.
[3] Wang B, Diao QY, Zhang ZY, et al. Antitumor activity of beepollen polysaccharides from Rosa Rugosa[J]. Molecular Medi-cine Reports, 2013, 7(5):1555-1558.
[4] Zhang X, Li SS, Sun L, et al. Further analysis of the structureand immunological activity of an RG-I type pectin from Panaxginseng[J].Carbohydrate Polymers, 2012, 89(2):519-525.
[5]王博,张梦珊,张中玉,等.菊花蜂花粉多糖的分离纯化及抗肿瘤活性研究[J].食品工业科技, 2016, 37(5):358-360,364.
[6] Wang J, Li SS, Fan YY, et al. Anti-fatigue activity of neutral andacidic polysaccharides from Panax ginseng C. A. Meyer[J].Journal of Ethenopharmacol, 2010, 130(2):421-423.
[7] Yu L, Zhang X, Li SS, et al. Rhamnogalacturonan I domainsfrom ginseng pectin[J]. Carbohydrate Polymers, 2010, 79(4):811-817.
[8]刘佳妮,王硕,白米雪,等.响应面法优化丹参药渣多糖提取工艺及其抗氧化活性研究[J].时珍国医国药, 2017, 28(2):454-457.
[9] Morrisn VJ, Belshaw NJ, Waldron KW, et al. The bioactivity ofmodied pectin fragments[J]. Bioactive Carbohydrate DietaryFibre, 2013, 1(1):21-37.
[10]孟思彤,徐艳芝,王振月.多糖的化学修饰对其生物活性影响研究进展[J].天然产物研究与开发, 2014, 26:1901-1905.
[11]李珊珊,金银萍,姚春林,等.人参多糖的结构与活性研究进展[J].中国中药杂志, 2014, 39(24):4709-4715.
[12] Liu J, Willfor S, Xu CL. A review of bioactive plant pol-ysaccharides:biological activities, functionalization, and bio-medical applications[J]. Bioactive Carbohydrate Dietary Fib-re, 2015,(5):31-61.