基于指纹图谱及化学识别模式的乌梅炮制品质量评价
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摘要
目的建立并比较乌梅不同炮制品的高效液相色谱(HPLC)法指纹图谱。方法通过HPLC法测定乌梅不同炮制品的HPLC指纹图谱,运用中药色谱指纹图谱相似度评价系统(2012版)和SPSS 22. 0统计软件进行相似度评价及主成分分析。结果通过指纹图谱与化学识别模式综合分析,乌梅不同炮制品均有25个指纹特征峰,乌梅及醋乌梅与对照指纹图谱相似度较高,乌梅炭相似度较低。结论该方法建立了乌梅不同炮制品的HPLC指纹图谱,稳定性良好,可为乌梅的质量控制及临床应用提供参考。
Objective To establish and compare the fingerprints of different processed products of Mume Fructus by HPLC. Methods The fingerprints of different processed products of Mume Fructus were determined by HPLC,the similarity was evaluated by the similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( version 2012),and the principal component analysis( PCA) was performed by the SPSS 22. 0 software. Results According to the comprehensive analysis of fingerprint and chemical pattern recognition,25 fingerprint characteristic peaks were found in the different processed products of Mume Fructus,the similarity of Mume Fructus and Mume Fructus processed with vinegar was higher,but the similarity of charcoal of Mume Fructus was lower. Conclusion The fingerprints of the different processed products of Mume Fructus are established. The method is stable,and it can provide reference for quality control and clinical application of Mume Fructus.
Objective To establish and compare the fingerprints of different processed products of Mume Fructus by HPLC. Methods The fingerprints of different processed products of Mume Fructus were determined by HPLC,the similarity was evaluated by the similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( version 2012),and the principal component analysis( PCA) was performed by the SPSS 22. 0 software. Results According to the comprehensive analysis of fingerprint and chemical pattern recognition,25 fingerprint characteristic peaks were found in the different processed products of Mume Fructus,the similarity of Mume Fructus and Mume Fructus processed with vinegar was higher,but the similarity of charcoal of Mume Fructus was lower. Conclusion The fingerprints of the different processed products of Mume Fructus are established. The method is stable,and it can provide reference for quality control and clinical application of Mume Fructus.
引文
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[2]刘梦茵,刘芳,周涛,等.乌梅提取物对蜡状芽孢杆菌的抑菌机理研究[J].食品科学,2012,33(1):103-105.
[3]耿飞,王伟,周涛.乌梅提取液对李斯特菌的抑菌机理[J].食品科学,2011,32(15):88-93.
[4]王小婷,蒋国政,王海滨,等.乌梅肉提取物抗腹泻作用研究[J].中国现代应用药学,2016,33(4):407-410.
[5]刘先琼,许腊英.多指标综合加权评分研究乌梅炭炮制工艺[J].中草药,2009,40(12):1898-1900.
[6]孙晓静,许腊英,陈智国,等.乌梅炭品乙酸乙酯部位特征指纹图谱研究[J].湖北中医药大学学报,2011,13(3):27-29.
[7]唐小龙,罗旭,陈波,等.不同产地贯叶金丝桃高效液相色谱指纹图谱研究[J].中国药业,2017,26(21):12-14.
[8]沈黎.醋蒸五味子高效液相色谱特征指纹图谱研究[J].中国药业,2017,26(16):13-14.
[9]吴玲芳,林琛,袁永兵,等.叶下珠指纹图谱及模式识别研究[J].中草药,2015,46(4):572-575.
[10]靳怡然,杜英峰,田婷婷,等. HPLC-PDA指纹图谱结合主成分分析评价不同产地冬凌草药材的质量[J].中草药,2015,46(15):2291-2295.
[11]魏屹,丁雪娇,宋良科,等. HPLC指纹图谱法研究峨眉黄连与三角叶黄连[J].华西药学杂志,2012,27(2):193-195.
[12]郑彩霞,郏文青,魏屹,等.甘青乌头的HPLC指纹图谱研究[J].华西药学杂志,2017,32(4):408-410.
[13]罗旭,唐小龙,杨宋琪,等.不同产地刺五加药材高效液相色谱指纹图谱研究[J].中国药业,2017,26(20):6-8.
[14]郭明里,张加余,王冰,等.川麦冬高效液相色谱指纹图谱研究[J].中国药业,2017,26(4):1-5.
[15]余鹏.乌梅饮片质量标准的规范化研究[D].武汉:湖北中医学院,2003.