苍术炒焦前后正丁醇部位对湿阻中焦证大鼠水盐代谢影响
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摘要
目的:通过研究苍术炒焦前后正丁醇部位对湿阻中焦证大鼠水盐代谢的药效,阐释焦苍术药效改变的机制,为临床合理用药提供客观依据。方法:以湿阻中焦证大鼠为模型,比较不同给药组大鼠血清醛固酮(ALD)、肾小管水孔蛋白2(AQP2)和血浆K~+、Na~+、Cl~-等电解质水平。结果:模型组大鼠血清ALD、肾小管AQP2与正常组相比明显升高,血浆K~+、Na~+和Cl~-浓度均有所降低,以K~+浓度降低较为显著;与模型组比较,各给药组均表现为ALD和AQP2不同程度降低,其中生品正丁醇部位高剂量组(正生高)对ALD和AQP2水平有显著性影响,且其调节血浆电解质水平能力与阳性组无显著性差异(P<0.05)。结论:苍术炒焦以后,燥性得以缓和,焦苍术对模型大鼠水盐代谢的调节作用明显减弱。
Objective:Through the study of n-butanol part extracted from Rhizoma Atractylodis before and after the deep-fired on the water and salt metabolism in rats with syndrome of accumulation of dampness in middle-Jiao,to interpret the mechanism of efficacy change for the focal Rhizoma Atractylodis and provide objective basis for clinical useing drugs reasonably.Methods:Syndrome of Accumulation of Dampness in Middle-Jiao model rats were established to compare each group′s different drug treatment on serum aldosterone(ALD),renal tubular hole protein 2(AQP2) and serum K~+,Na~+,Cl~- electrolyte levels.Results:Compared with normal group,serum ALD and renal tubular AQP2 increased significantly in the model group.At the same time,the concentrations of K~+,Na~+ and Cl– in plasma were all reduced,but the K~+ concentration reduced considerably.Compared with the model group,the levels of ALD and AQP2 were reduced in each group for different drug treatments.Particularly,the high dose group′s extract from raw product had significant difference compared with the model group on impacting on the ALD and AQP2 levels.There was no significant difference in plasma electrolyte levels to the positive group also(P<0.05).Conclusion:After deep-fired,the dry temper of Rhizoma Atractylodis has been eased and its adjusting ability reduced significantly on the water and salt metabolism in rats with syndrome of accumulation of dampness in middle-Jiao.
Objective:Through the study of n-butanol part extracted from Rhizoma Atractylodis before and after the deep-fired on the water and salt metabolism in rats with syndrome of accumulation of dampness in middle-Jiao,to interpret the mechanism of efficacy change for the focal Rhizoma Atractylodis and provide objective basis for clinical useing drugs reasonably.Methods:Syndrome of Accumulation of Dampness in Middle-Jiao model rats were established to compare each group′s different drug treatment on serum aldosterone(ALD),renal tubular hole protein 2(AQP2) and serum K~+,Na~+,Cl~- electrolyte levels.Results:Compared with normal group,serum ALD and renal tubular AQP2 increased significantly in the model group.At the same time,the concentrations of K~+,Na~+ and Cl– in plasma were all reduced,but the K~+ concentration reduced considerably.Compared with the model group,the levels of ALD and AQP2 were reduced in each group for different drug treatments.Particularly,the high dose group′s extract from raw product had significant difference compared with the model group on impacting on the ALD and AQP2 levels.There was no significant difference in plasma electrolyte levels to the positive group also(P<0.05).Conclusion:After deep-fired,the dry temper of Rhizoma Atractylodis has been eased and its adjusting ability reduced significantly on the water and salt metabolism in rats with syndrome of accumulation of dampness in middle-Jiao.
引文
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[12] 黄秀深,刘德芳,陈杰.大鼠中焦湿阻证与抗利尿激素关系的实验研究[J].成都中医药大学学报,2002,25(1):37.
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[14] 伍小燕,陈朝,张过伟,等.泽泻水提物对正常利尿活性及肾脏髓质AQP2作用的研究[J].实用临床医学杂志,2010,14(21):5.
[15] 高清华,吕文亮,李华锋,等.燥湿运脾合剂对小鼠水液代谢影响的实验研究[J].湖北中医杂志,2007,29(11):5-6.
[16] Cuesta A,Vargas-Chacoff L,Arjona F,et al.Effect of sexsteroid hormones′ testosterone and estradiol on humoral immune parameters of gilthead seabream[J].Fish Shellfish Immunol,2007,23(3):693-700.
[17] 季光琼,肖波,刘艳菊,等.苍术麸炒前后正丁醇部位对湿阻中焦证大鼠的药效学研究[J].中成药,2014,36(7):1527-1529.
[2] 龚千锋.中药炮制学[M].9版.北京:中国中医药出版社,2012:161.
[3] 吴慧,赵文龙,单国顺,等.白术茯苓汤中用生白术或熟白术对湿困脾阻模型大鼠的影响[J].中国药房,2014,25(7):587-589.
[4] 汪滢,孙宏文.湿阻中焦证现代机制研究[J].长春中医药大学学报,2015,31(2):297-300.
[5] 刘德芳,黄秀深,张丰华,等.大鼠湿阻中焦证的水盐代谢调节机制及平胃散对其的影响[J].中国中医药信息杂志,2005,12(1):26-27.
[6] 付伟,孙雄杰,李水清,等.苍术炒焦前后对湿阻中焦模型大鼠AQP2,AQP3含量的影响[J].中国实验方剂学杂志,2016,22(19):19-22.
[7] 湖北省食品药品监督管理局.湖北省中药饮片炮制规范:一部[S].武汉:湖北科学技术出版社,2009.
[8] 陈奇.中药药理研究方法学[M].3版.北京:人民卫生出版社,2011:1262.
[9] 刘艳菊,陈雯雯,曾敏,等.苍术炮制前后水提物药效学研究[J].中国中药杂志,2012,37(15):2276-2279.
[10] 王海英,高宇,高长斌,等.水通道蛋白1、3在糖尿病心肌病大鼠心肌组织的表达及意义[J].中国老年学杂志,2010,30(7):953-954.
[11] 张丰华,黄秀深.平胃散对湿阻中焦模型醛固酮和神经降压素的影响[J].时珍国医国药,2008,19(11):2781-2782.
[12] 黄秀深,刘德芳,陈杰.大鼠中焦湿阻证与抗利尿激素关系的实验研究[J].成都中医药大学学报,2002,25(1):37.
[13] Laforenza U.Water channel proteins in the gastrointestinal tract[J].Mol Aspects Med,2012,33(5/6):642-650.
[14] 伍小燕,陈朝,张过伟,等.泽泻水提物对正常利尿活性及肾脏髓质AQP2作用的研究[J].实用临床医学杂志,2010,14(21):5.
[15] 高清华,吕文亮,李华锋,等.燥湿运脾合剂对小鼠水液代谢影响的实验研究[J].湖北中医杂志,2007,29(11):5-6.
[16] Cuesta A,Vargas-Chacoff L,Arjona F,et al.Effect of sexsteroid hormones′ testosterone and estradiol on humoral immune parameters of gilthead seabream[J].Fish Shellfish Immunol,2007,23(3):693-700.
[17] 季光琼,肖波,刘艳菊,等.苍术麸炒前后正丁醇部位对湿阻中焦证大鼠的药效学研究[J].中成药,2014,36(7):1527-1529.