小檗碱与多种成分复配及其对番茄灰霉病的抑制作用
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摘要
采用菌丝生长速率法和共毒因子法,测定小檗碱与山豆根、炉甘石、芸薹素内酯、复硝酚钠复配剂对番茄灰霉病的抑制作用。结果表明,小檗碱(0.280 mg/mL)与10%山豆根(2.257mg/mL)、小檗碱(0.1555mg/mL)与炉甘石(0.062 5 mg/mL)复配使用对灰霉病菌抑制效果较佳,小檗碱与芸薹素内酯、复硝酚钠复配能促进灰霉菌生长。电镜观察发现,经小檗碱和山豆根、炉甘石复配处理的灰霉病菌菌丝扭曲、干瘪。田间防效试验结果表明,10%小檗碱可湿性粉剂1 000倍稀释液对灰霉病菌的防效为71.7%,小檗碱与山豆根、炉甘石、复硝酚钠、芸薹素内酯合用均能增加小檗碱对番茄灰霉病的防效。由此可知,小檗碱可与山豆根、炉甘石、芸薹素内酯、复硝酚钠复配用于番茄灰霉病防治。
The antibacterial activity of berberine with radix Sophorae tonkinensis,calamine,brassinolide and compound sodium nitrophenolate on Botrytis cinerea was studied by the method of mycelial growth rate and co-toxicity factor. The results showed that both of complex of berberine(0.280 mg/mL) and radix Sophorae tonkinensis(2.257 mg/mL) or berberine(0.155 5mg/mL) and calamina(0.062 5 mg/mL) had strong inhibition against B. cinerea. However, the mixtures of berberine and brassinolide or compound sodium nitrophenolate could promote the growth of B. cinerea. Scanning electron microscope analysis revealed that the mycelia of B. cinerea manifested abnormity including distortion and collapse. The results of field control experiments showed that the control effect of 10% berberine was 71.7%. Furthermore, berberine combined with radix Sophorae tonkinensis, calamina, brassinolide and compound sodium nitrophenolate could increase the control effect of berberine against Botrytis cinereal. So it could be hypothesized that complex drugs could inhibited the gray mold cell membrane and played an antibacterial effect.
The antibacterial activity of berberine with radix Sophorae tonkinensis,calamine,brassinolide and compound sodium nitrophenolate on Botrytis cinerea was studied by the method of mycelial growth rate and co-toxicity factor. The results showed that both of complex of berberine(0.280 mg/mL) and radix Sophorae tonkinensis(2.257 mg/mL) or berberine(0.155 5mg/mL) and calamina(0.062 5 mg/mL) had strong inhibition against B. cinerea. However, the mixtures of berberine and brassinolide or compound sodium nitrophenolate could promote the growth of B. cinerea. Scanning electron microscope analysis revealed that the mycelia of B. cinerea manifested abnormity including distortion and collapse. The results of field control experiments showed that the control effect of 10% berberine was 71.7%. Furthermore, berberine combined with radix Sophorae tonkinensis, calamina, brassinolide and compound sodium nitrophenolate could increase the control effect of berberine against Botrytis cinereal. So it could be hypothesized that complex drugs could inhibited the gray mold cell membrane and played an antibacterial effect.
引文
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[18]汪晓红,宁伟文,赵伟,等.4种药剂对大豆健康和产量影响的比较研究[J].农药科学与管理,2017,38(8):48-53.
[19] TANG L,DONG L N,PENG X J,et al. Pharmacokinetic characterization of oxymatrine and matrine in rats after oral administration of radix Sophorae tonkinensis extract and oxymatrine by sensitive and robust UPLC-MS/MS method[J].Journal of Pharmaceutical and Biomedical Analysis,2013,83:179-185.
[20]蔡锦源,韦坤华,熊建文,等.山豆根黄酮的提取及抗氧化抑菌活性[J].精细化工,2017,34(3):285-293.
[21]裴国献,顾立强,于立新,等.异体手移植二例报告[J].中华医学杂志,2000,80(6):16-20.
[2]纪军建,张小风,王文桥,等.番茄灰霉病防治研究进展[J].中国农学通报,2012,28(31):109-113.
[3]刘长令,翟煜翥,张运晓,等.防治灰霉病用杀菌剂的开发[J].农药,2000,39(3):1-6.
[4] VAN ASSELT E D,VAN DER SPIEGEL M, NOORDAM M Y,et al. Risk ranking of chemical hazards in food—A case study on antibiotics in the Netherlands[J].Food Research International,2013,54(2):1636-1642.
[5]陈晓明,王程龙,薄瑞.中国农药使用现状及对策建议[J].农药科学与管理,2016,37(2):4-8.
[6]王文桥,马平,韩秀英,等.生物源杀菌剂与化学药剂协调防控番茄病害[J].中国生物防治学报,2011,27(1):104-109,49.
[7]束长龙,曹蓓蓓,袁善奎,等.微生物农药管理现状与展望[J].中国生物防治学报,2017,33(3):297-303.
[8]李波,朱维良,陈凯先.小檗碱及其衍生物的研究进展[J].药学学报,2008,43(8):773-787.
[9] HE C M,CHENG Z H,CHEN D F. Qualitative and quantitative analysis of flavonoids in Sophora tonkinensis by LC/MS and HPLC[J].Chinese Journal of Natural Medicines,2013,11(6):690-698.
[10]杨连菊,张志杰,李娆娆,等.中药炉甘石的成分分析[J].中国中药杂志,2012,37(3):331-334.
[11]蒋卫杰.有机生态型无土栽培番茄营养生理与优化施肥研究[D].北京:中国农业科学院,2007.
[12]孙惠敏,李保同,郭明程,等.几种杀菌剂对柑桔溃疡病的生物活性[J].江西农业大学学报,2011,33(1):38-42.
[13] HOU D Y,YAN C Q,LIU H X,et al. Berberine as a natural compound inhibits the development of brown rot fungus Monilinia fructicola[J].Crop Protection,2010,29(9):979-984.
[14]裴庆慧,王玥,刘宇莹,等.五种中药材乙醇提取物对植物病原真菌的抑制作用研究[J].食品工业科技,2016,37(15):90-95.
[15]纪明山,祁之秋,王英姿,等.番茄灰霉病菌对嘧霉胺的抗药性[J].植物保护学报,2003,30(4):396-400.
[16]陈立,徐汉虹,李云宇,等.农药复配最佳增效配方筛选方法的探讨[J].植物保护学报,2000,27(4):349-354.
[17]张秀珍.农业部发布新的农药田间药效试验准则[J].农化新世纪,2008(2):13.
[18]汪晓红,宁伟文,赵伟,等.4种药剂对大豆健康和产量影响的比较研究[J].农药科学与管理,2017,38(8):48-53.
[19] TANG L,DONG L N,PENG X J,et al. Pharmacokinetic characterization of oxymatrine and matrine in rats after oral administration of radix Sophorae tonkinensis extract and oxymatrine by sensitive and robust UPLC-MS/MS method[J].Journal of Pharmaceutical and Biomedical Analysis,2013,83:179-185.
[20]蔡锦源,韦坤华,熊建文,等.山豆根黄酮的提取及抗氧化抑菌活性[J].精细化工,2017,34(3):285-293.
[21]裴国献,顾立强,于立新,等.异体手移植二例报告[J].中华医学杂志,2000,80(6):16-20.