长白落叶松离体再生体系的建立
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摘要
以长白落叶松成熟合子胚诱导出的不定芽为外植体,通过筛选不定芽增殖的最适培养基及激素配比、探讨不同浓度赤霉素(GA_3)及活性炭(AC)对不定芽伸长的作用以及比较不同处理对试管内及试管外生根的影响,最终建立离体再生体系。结果表明:BM+1.0 mg/L TDZ时,不定芽增殖最快,增殖系数为5.0;GA_3对不定芽的伸长有毒害,2.0 g/L活性炭(AC)对不定芽伸长促进作用明显,伸长比率达262%;1/2BM+0.5 mg/L IBA+1.0 mg/L NAA为试管内生根的最佳组合,生根率达26%,不定芽在750 mg/L NAA中浸泡25 s,试管外生根率(37%)最高。
The adventitious buds induced from mature zygotic embryos of Larix olgensis were used as explants. Screened optimum medium and hormone ratio for adventitious bud proliferation, discussed the effects of different concentrations of gibberellin(GA_3) and activated carbon(AC) on elongation of adventitious buds, and effects of different treatments on rooting outside of the test tube and in vitro, finally, regeneration system was established. The results showed that: When it was cultured on BM with 1.0 mg/L TDZ, the proliferation coefficient reached the highest by 5.0; GA_3 had toxic on effects elongation of adventitious buds, but 2.0 g/L activated carbon had a good effect on it and the elongation rate reached 262%; When it was cultured on 1/2 BM medium with 0.5 mg/L IBA and1.0 mg/L NAA for the in vitro rooting, the rooting rate was 26%, but tube external rooting by dipping 25 seconds with 750 mg/L NAA was the highest, up to 37%.
The adventitious buds induced from mature zygotic embryos of Larix olgensis were used as explants. Screened optimum medium and hormone ratio for adventitious bud proliferation, discussed the effects of different concentrations of gibberellin(GA_3) and activated carbon(AC) on elongation of adventitious buds, and effects of different treatments on rooting outside of the test tube and in vitro, finally, regeneration system was established. The results showed that: When it was cultured on BM with 1.0 mg/L TDZ, the proliferation coefficient reached the highest by 5.0; GA_3 had toxic on effects elongation of adventitious buds, but 2.0 g/L activated carbon had a good effect on it and the elongation rate reached 262%; When it was cultured on 1/2 BM medium with 0.5 mg/L IBA and1.0 mg/L NAA for the in vitro rooting, the rooting rate was 26%, but tube external rooting by dipping 25 seconds with 750 mg/L NAA was the highest, up to 37%.
引文
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[10]Van Nieunkerk S P, Zimmerman R H, Ford haml.Hortscience, 1986, 21:516-518.
[11]王光萍,黄敏仁.福建山樱花的组织培养及植株再生[J].南京林业大学学报, 2002, 26(2):73-75.
[12]阙国宁,房建军,葛万川,张守英,毛秋娟.火炬松、湿地松、晚松组培繁殖的研究[J].林业科学研究,1997,10(03):4-9.
[13]黄健秋,卫志明.松属树种的组织培养和原生质体培养[J].植物学通报, 1994, 11(1):34-42.
[14]Jang JC, Tainter FH. Micro propagation of shortleaf Virginia and loblolly X. shortleaf pine hybrids via organogenesis. Plant Cell[J].Tissue and Organ Culture,1991, 25(1):61-67.
[15]Henrique A, Campinhos E N, Ono E O, et al. Effect of plant growth regulators in the rooting of Pinus cuttings[J]. Braz Arch Biol Tech, 2006, 49:189-196.
[2]徐悦丽.长白落叶松群体遗传变异及4CL基因单核苷酸多态性的研究[D].黑龙江:东北林业大学, 2012,1-2.
[3]李际红,邢世岩,周继磊,李建华,唐海霞.黑赤松成熟胚诱导不定芽的研究[J].中国农学通报,2010,26(01):22-26.
[4]吴克贤,李伟,徐妙珍,党常顺,刘越.长白落叶松组织培养的研究[J].林业科学,1996,(02):125-133.
[5]王伟达,李成浩,张含国,刘宝光,庞晓峰.长白落叶松愈伤组织诱导与不定芽分化[J].东北林业大学学报,2008,(02):5-7.
[6]王伟达.落叶松体细胞胚形成与不定芽诱导的研究[D].东北林业大学,2008.
[7]吴丽君.湿地松、火炬松离体培养植株再生技术的研究[D].南京:南京林业大学森林保护学, 2008.
[8]黄健秋,卫志明.松属树种的组织培养和原生质体培养[J].植物学通报, 1994, 11(1):34-42.
[9]张小红,张红燕,武军,闵东红,康冰,李军超.TDZ对香椿愈伤组织诱导及芽增殖生长等的影响[J].西北农林科技大学学报(自然科学版),2002,(05):35-39.
[10]Van Nieunkerk S P, Zimmerman R H, Ford haml.Hortscience, 1986, 21:516-518.
[11]王光萍,黄敏仁.福建山樱花的组织培养及植株再生[J].南京林业大学学报, 2002, 26(2):73-75.
[12]阙国宁,房建军,葛万川,张守英,毛秋娟.火炬松、湿地松、晚松组培繁殖的研究[J].林业科学研究,1997,10(03):4-9.
[13]黄健秋,卫志明.松属树种的组织培养和原生质体培养[J].植物学通报, 1994, 11(1):34-42.
[14]Jang JC, Tainter FH. Micro propagation of shortleaf Virginia and loblolly X. shortleaf pine hybrids via organogenesis. Plant Cell[J].Tissue and Organ Culture,1991, 25(1):61-67.
[15]Henrique A, Campinhos E N, Ono E O, et al. Effect of plant growth regulators in the rooting of Pinus cuttings[J]. Braz Arch Biol Tech, 2006, 49:189-196.