藏药二十五味儿茶凝胶对大鼠慢性湿疹模型LTB-4、LTC-4因子的影响
|
摘要
目的评价藏药二十五味儿茶凝胶治疗大鼠慢性湿疹的疗效。方法采用2,4-二硝基氯苯(DNCB)诱导大鼠背部慢性湿疹。将72只SD大鼠随机分为两组,取12只大鼠作为空白对照组,另一组共60只造模成功后随机分为模型组、阳性对照组、藏药二十五味儿茶凝胶高、中、低剂量组5组。阳性对照组给予丁酸氢化可的松乳膏(尤卓尔),藏药二十五味儿茶凝胶低(0.14g/kg)、中(0.21g/kg)、高(0.35g/kg)剂量经皮给药,于致敏后给药,观察各组大鼠第4次激发3天后采用ELISA法检测大鼠血清中的LTB-4、LTC-4的含量。结果藏药二十五味儿茶凝胶中剂量能有效减轻慢性湿疹大鼠引起的皮损炎症程度,调节慢性湿疹组大鼠血清中LTB-4和LTC-4水平。结论藏药二十五味儿茶凝胶能降低慢性湿疹模型大鼠血清LTB-4和LTC-4含量,其治疗湿疹的作用机制可能与免疫调节及减轻炎症反应相关。
Objective To evaluate the therapeutic effect of Tibetan drug Ershiwuweiercha gel on chronic eczema in model rats. Methods A total of 72 SD rats with chronic eczema induced by 2,4-dinitrochlorobenzene( DNCB) were randomly divided into two groups: blank control group( n = 12),and the other group consisting of 60 successfully model-made rats was randomly subdivided into model group,positive control group,high,medium and low dose of Tibetan drug Ershiwuweiercha gel group. Positive control group was given hydrocortisone butyrate cream( Youzhuoer),low-dose( 0. 14 g/kg),medium-dose( 0. 21 g/kg),and high-dose( 0. 35 g/kg) group were treated with percutaneously absorbed drugs after sensitization. Three days after observation of the fourth challenge in each group,serum LTB-4 and LTC-4 levels were detected by ELISA. Results The medium-dose Tibetan drug Ershiwuweiercha gel was effective in the amelioration of skin inflammation caused by rats with chronic eczema,and as well as in the adjustment of the levels of ltb-4 and ltc-4 in the serum of rats with chronic eczema. Conclusion Serum ltb-4 and ltc-4 levels in rats with chroniceczema can be decreased by Tibetan drug Ershiwuweiercha gel,and its mechanism may be associated with immune regulation and reducing inflammatory response.
Objective To evaluate the therapeutic effect of Tibetan drug Ershiwuweiercha gel on chronic eczema in model rats. Methods A total of 72 SD rats with chronic eczema induced by 2,4-dinitrochlorobenzene( DNCB) were randomly divided into two groups: blank control group( n = 12),and the other group consisting of 60 successfully model-made rats was randomly subdivided into model group,positive control group,high,medium and low dose of Tibetan drug Ershiwuweiercha gel group. Positive control group was given hydrocortisone butyrate cream( Youzhuoer),low-dose( 0. 14 g/kg),medium-dose( 0. 21 g/kg),and high-dose( 0. 35 g/kg) group were treated with percutaneously absorbed drugs after sensitization. Three days after observation of the fourth challenge in each group,serum LTB-4 and LTC-4 levels were detected by ELISA. Results The medium-dose Tibetan drug Ershiwuweiercha gel was effective in the amelioration of skin inflammation caused by rats with chronic eczema,and as well as in the adjustment of the levels of ltb-4 and ltc-4 in the serum of rats with chronic eczema. Conclusion Serum ltb-4 and ltc-4 levels in rats with chroniceczema can be decreased by Tibetan drug Ershiwuweiercha gel,and its mechanism may be associated with immune regulation and reducing inflammatory response.
引文
[1]阎春林,廖康煌.白三烯在特应性皮炎和慢性荨麻疹发病中的作用[J].临床皮肤科杂志,2003,32(10):625-626.
[2]Relly DM,Parslew R,Sharpe GR,el al.Inflammatory mediators in normal sensitive and diseased skin type[J].Acta Derm Venereol,2000,80(3):171-174.
[3]钟华,郝飞,向明明.湿疹患者血清和皮损内白三烯水平测定[J].中华皮肤科杂志,2005,38(11):702-703.
[4]孙明立,魏敏杰,金万宝,等.两种变应性接触性皮炎动物模型的建立及比较[J].中国实验动物学报,2008,12(2):132.
[5]Malajian D,Belsito DV.Cutaneous delayed-type hypersensitivityin patients with atopic dermatitis[J].J Am Acad Dermatol,2013,69(2):232-237.
[6]梁伟海,黄鹏,李伦,等.苦桉祛疹凝胶对大鼠湿疹模型的影响[J].中国实验方剂学杂志,2016,22(13):97-101.
[7]胡仕宏,宋秋荷,周会祥.湿疹患者血清、皮损和尿液中白三烯以及细胞因子水平的测定[J].西南国防医药,2009,19(6):567-569.
[2]Relly DM,Parslew R,Sharpe GR,el al.Inflammatory mediators in normal sensitive and diseased skin type[J].Acta Derm Venereol,2000,80(3):171-174.
[3]钟华,郝飞,向明明.湿疹患者血清和皮损内白三烯水平测定[J].中华皮肤科杂志,2005,38(11):702-703.
[4]孙明立,魏敏杰,金万宝,等.两种变应性接触性皮炎动物模型的建立及比较[J].中国实验动物学报,2008,12(2):132.
[5]Malajian D,Belsito DV.Cutaneous delayed-type hypersensitivityin patients with atopic dermatitis[J].J Am Acad Dermatol,2013,69(2):232-237.
[6]梁伟海,黄鹏,李伦,等.苦桉祛疹凝胶对大鼠湿疹模型的影响[J].中国实验方剂学杂志,2016,22(13):97-101.
[7]胡仕宏,宋秋荷,周会祥.湿疹患者血清、皮损和尿液中白三烯以及细胞因子水平的测定[J].西南国防医药,2009,19(6):567-569.