游泳运动可抑制HCFD诱导肥胖大鼠模型凝血功能异常
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摘要
目的明确游泳运动是否可抑制高碳水化合物和脂肪饮食(HCFD)诱导肥胖大鼠凝血功能异常。方法 40只雄性Wistar大鼠被随机分为4组,对照组、对照+游泳组、HCFD组和HCFD+游泳组。各组大鼠均饲养15周,其中对照组大鼠用标准实验室饲料喂养15周;HCFD组大鼠HCFD饲养15周。游泳组大鼠在第11周到第15周进行游泳训练。然后分别对各组大鼠炎性标志物和凝血相关指标等进行检测。结果 HCFD导致大鼠血糖、IR、血脂异常和炎性生物标志物显著增高。此外,HCFD显著增加大鼠凝血和血栓形成生物标志物,如纤维蛋白原、纤溶酶原激活剂抑制物-1、凝血酶原时间、活化部分凝血活酶时间、凝血和出血时间以及血小板聚集等,而游泳运动可抑制HCFD大鼠上述指标的增加。结论在肥胖和IR大鼠模型中,通过游泳运动可以改善凝血功能异常。
Objective To determine whether swim exercise can inhibit high carbohydrate and fat diet(HCFD)-induced biomarkers of coagulation. Methods 40 male Wistar rats were randomly divided into 4 groups: control group, control + swimming group, HCFD group, and HCFD+ swimming group. Rats of control group were fed with standard laboratory chow for 15 weeks, and rats of HCFD group were fed HCFD for 15 weeks. Swim exercise-‘treated' rats started swim exercise training from the 11~(th) week until being sacrificed, on week 15.Then inflammatory markers and coagulation related indicators were detected in each group. Results HCFD caused a significant increase in blood glucose, insulin resistance(IR), lipidemia, and inflammatory biomarkers. In addition, HCFD significantly modulated coagulation and thrombosis biomarkers; fibrinogen, plasminogen activator inhibitor-1, prothrombin time, activated partial thromboplastin time, blood clotting and bleeding time, and platelet aggregation that was effectively inhibited by swimming exercises. Conclusions In an animal model of obesity and IR, there is a significant change in hemostasis, which is ameliorated by swim exercise.
Objective To determine whether swim exercise can inhibit high carbohydrate and fat diet(HCFD)-induced biomarkers of coagulation. Methods 40 male Wistar rats were randomly divided into 4 groups: control group, control + swimming group, HCFD group, and HCFD+ swimming group. Rats of control group were fed with standard laboratory chow for 15 weeks, and rats of HCFD group were fed HCFD for 15 weeks. Swim exercise-‘treated' rats started swim exercise training from the 11~(th) week until being sacrificed, on week 15.Then inflammatory markers and coagulation related indicators were detected in each group. Results HCFD caused a significant increase in blood glucose, insulin resistance(IR), lipidemia, and inflammatory biomarkers. In addition, HCFD significantly modulated coagulation and thrombosis biomarkers; fibrinogen, plasminogen activator inhibitor-1, prothrombin time, activated partial thromboplastin time, blood clotting and bleeding time, and platelet aggregation that was effectively inhibited by swimming exercises. Conclusions In an animal model of obesity and IR, there is a significant change in hemostasis, which is ameliorated by swim exercise.
引文
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[12] 王庆福,甘春龙,衣雪洁.游泳对高脂膳食诱导肥胖和肥胖抵抗模型大鼠的作用[J].中国组织工程研究,2016,20(27):4083-4089.
[13] MIN H K,LEE J,JUNG S,et al.The involvement of ginseng berry extract in blood flow via regulation of blood coagulation in rats fed a high-fat diet[J].Journal of Ginseng Research,2017,41(2):120.
[14] HEBER S,VOLF I.Effects of physical (in)activity on platelet function[J].Biomed Research International,2015(3):165078.
[15] EFB C,BONFIM M R,TURI B C,et al.Effect of moderate-intensity exercise on inflammatory markers among postmenopausal women[J].J Phys Act Health,2017,14(6):479-485.
[16] SATCHELL M D,MANN K J,BALAGOPAL P.Do obese children have chronic inflammation & could this contribute to future CVD risk?[J].Current Cardiovascular Risk Reports,2012,6(6):579-590.
[2] KLüNDERKLüNDER M,CRUZ M,GARCíAMACEDO R,et al.Inflammatory cytokines adiponectin,resistin,IL-6 and IFN-γ are associated with insulin resistance in eutrophic and obese children[J].Boletín Médico Del Hospital Infantil De México,2014,71(1):8-14.
[3] BADIMON L,HERNNDEZ V R,VILAHUR G.Atherothrombotic risk in obesity[J].Hamostaseologie,2013,33(4):259-268.
[4] KOTRONEN A,JOUTSI-KORHONEN L,SEVASTIANOVA K,et al.Increased coagulation factor VIII,IX,XI and XII activities in non-alcoholic fatty liver disease[J].Liver International,2011,31(2):176-183.
[5] TARGHER G,ZOPPINI G,MOGHETTI P,et al.Disorders of coagulation and hemostasis in abdominal obesity:emerging role of fatty liver[J].Seminars in Thrombosis & Hemostasis,2010,31(1):41-48.
[6] LEMOS E T D ,FLáVIO R,BAPTISTA S,et al.Exercise training is associated with improved levels of C-reactive protein and adiponectin in ZDF (type 2) diabetic rats[J].Med Sci Monit,2007,13(8):168-174.
[7] BARAZZONI R,DEUTZ N E P,BIOLO G,et al.Carbohydrates and insulin resistance in clinical nutrition:Recommendations from the ESPEN expert group[J].Clinical Nutrition,2016,36(2):355-363.
[8] BARCLAY A W,BRAND-MILLER J.The Australian paradox:a substantial decline in sugars intake over the same timeframe that overweight and obesity have increased[J].Nutrients,2014,6(2):663-664.
[9] 杨莹,王薇薇,李爱科,等.高脂饲粮和高碳水化合物饲粮对大鼠脂肪代谢的影响[J].动物营养学报,2017,29(7):2603-2612.
[10] 李良鸣,杨则宜,魏源,等.运动后前糖原和大糖原的恢复规律及其机制研究[J].体育科学,2005,25(7):25-30.
[11] KO I G,JEONG J W,KIM Y H,et al.Aerobic exercise affects myostatin expression in aged rat skeletal muscles:a possibility of antiaging effects of aerobic exercise related with pelvic floor muscle and urethral rhabdosphincter[J].Int Neurourol J,2014,18(2):77-85.
[12] 王庆福,甘春龙,衣雪洁.游泳对高脂膳食诱导肥胖和肥胖抵抗模型大鼠的作用[J].中国组织工程研究,2016,20(27):4083-4089.
[13] MIN H K,LEE J,JUNG S,et al.The involvement of ginseng berry extract in blood flow via regulation of blood coagulation in rats fed a high-fat diet[J].Journal of Ginseng Research,2017,41(2):120.
[14] HEBER S,VOLF I.Effects of physical (in)activity on platelet function[J].Biomed Research International,2015(3):165078.
[15] EFB C,BONFIM M R,TURI B C,et al.Effect of moderate-intensity exercise on inflammatory markers among postmenopausal women[J].J Phys Act Health,2017,14(6):479-485.
[16] SATCHELL M D,MANN K J,BALAGOPAL P.Do obese children have chronic inflammation & could this contribute to future CVD risk?[J].Current Cardiovascular Risk Reports,2012,6(6):579-590.