肉苁蓉多糖对D-半乳糖致衰老小鼠抗疲劳作用及机制研究
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摘要
目的研究肉苁蓉多糖(CDP)对D-半乳糖致衰老小鼠抗疲劳的作用及其机制。方法将80只昆明种小鼠随机分为正常对照组、模型组、CDP低剂量组和CDP高剂量组,每组20只。模型组、CDP低剂量组和CDP高剂量组予D-半乳糖100 mg/kg颈背部皮下注射造模,正常对照组予等剂量0. 9%氯化钠注射液颈背部皮下注射,连续30 d。造模同时,CDP低剂量组及CDP高剂量组分别予CDP 100、400 mg/kg灌胃,对照组和模型组予100 mg/kg蒸馏水灌胃,每日1次,连续30 d。比较4组小鼠负重游泳时间,血清尿素氮(BUN)、乳酸(LA)水平,肝糖原、肌糖原含量,肝组织超氧化物歧化酶(SOD)、丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH-PX)活性。结果模型组小鼠负重游泳时间少于正常对照组(P <0. 05);与模型组相比,CDP低剂量组及CDP高剂量组小鼠负重游泳时间明显增加(P <0. 05)。模型组小鼠血清BUN及LA水平明显高于正常对照组(P <0. 05);与模型组相比,CDP低剂量组及CDP高剂量组小鼠血清BUN及LA水平明显降低(P <0. 05)。模型组肝糖原及肌糖原含量明显低于正常对照组(P <0. 05);与模型组相比,CDP低剂量组及CDP高剂量组肝糖原及肌糖原含量明显升高(P <0. 05)。与正常对照组相比,模型组小鼠肝组织SOD及GSH-PX活性降低(P <0. 05),MDA活性升高(P <0. 05);与模型组相比,CDP低剂量组及CDP高剂量组小鼠肝组织SOD及GSH-PX活性升高(P <0. 05),MDA活性降低(P <0. 05)。结论 CDP对D-半乳糖致衰老小鼠具有抗疲劳的作用,这种作用可能与降低血清BUN、LA含量,增加肝糖原、肌糖原含量,增强抗自由基损伤有关。
Objective To explore the anti-fatigue effect and mechanism of cistanche deserticola polysaccharide( CDP) on D-galactose-induced aging mice. Methods 80 Kunming mice were randomly divided into normal control group,model group,CDP low-dose group and CDP high-dose group,with 20 rats in each group.The model group,CDP low-dose group and CDP high-dose group were given D-galactose 100 mg/kg for the injection of the back of the neck,and the control group was injected subcutaneously with the same dose of 0. 9% sodium chloride injection for 30 days. At the same time,the CDP low-dose group and the CDP high-dose group were given CDP 100 and 400 mg/kg by gavage,respectively. the control group and the model group were given 100 mg/kg distilled water by gavage. All groups were treated once a day for 30 days. The heavy swimming time,the levels of serum urea nitrogen( BUN) and lactic acid( LA) in serum,the hepatic glycogen and muscle glycogen content,liver tissue superoxide dismutase( SOD),malondialdehyde( MDA)and glutathione peroxidase( GSH-PX) activities were compared between the four groups. Results The weight-bearing swimming time of the model group was lower than that of the control group( P < 0. 05). Compared with the model group,the weight-bearing swimming time was significantly increased in CDP low-dose group and CDP high-dose group( P < 0. 05). The serum BUN and LA levels in the model group were significantly higher than those in the control group( P < 0. 05). Compared with the model group,serum BUN and LA levels were significantly decreased in CDP low-dose group and CDP high-dose group( P < 0. 05). The hepatic glycogen and muscle glycogen content in the model group were significantly lower than those in the control group( P < 0. 05). Compared with the model group,the hepatic glycogen and muscle glycogen content were significantly decreased in CDP low-dose group and CDP high-dose group( P < 0. 05). Compared with the control group,the SOD and GSH-PX activities in the liver of the model group were decreased( P < 0. 05),and the MDA activity was increased( P < 0. 05). Compared with the model group,the activity of SOD and GSH-PX in liver tissue were increased in CDP low-dose group and CDP high-dose group( P < 0. 05),and MDA activity was decreased( P < 0. 05). Conclusion The CDP has anti-fatigue effect on aging mice induced by D-galactose. This effect may be related to the decrease of BUN and LA in serum,the increase of liver glycogen and muscle glycogen,and the enhancement of anti-free radical damage.
Objective To explore the anti-fatigue effect and mechanism of cistanche deserticola polysaccharide( CDP) on D-galactose-induced aging mice. Methods 80 Kunming mice were randomly divided into normal control group,model group,CDP low-dose group and CDP high-dose group,with 20 rats in each group.The model group,CDP low-dose group and CDP high-dose group were given D-galactose 100 mg/kg for the injection of the back of the neck,and the control group was injected subcutaneously with the same dose of 0. 9% sodium chloride injection for 30 days. At the same time,the CDP low-dose group and the CDP high-dose group were given CDP 100 and 400 mg/kg by gavage,respectively. the control group and the model group were given 100 mg/kg distilled water by gavage. All groups were treated once a day for 30 days. The heavy swimming time,the levels of serum urea nitrogen( BUN) and lactic acid( LA) in serum,the hepatic glycogen and muscle glycogen content,liver tissue superoxide dismutase( SOD),malondialdehyde( MDA)and glutathione peroxidase( GSH-PX) activities were compared between the four groups. Results The weight-bearing swimming time of the model group was lower than that of the control group( P < 0. 05). Compared with the model group,the weight-bearing swimming time was significantly increased in CDP low-dose group and CDP high-dose group( P < 0. 05). The serum BUN and LA levels in the model group were significantly higher than those in the control group( P < 0. 05). Compared with the model group,serum BUN and LA levels were significantly decreased in CDP low-dose group and CDP high-dose group( P < 0. 05). The hepatic glycogen and muscle glycogen content in the model group were significantly lower than those in the control group( P < 0. 05). Compared with the model group,the hepatic glycogen and muscle glycogen content were significantly decreased in CDP low-dose group and CDP high-dose group( P < 0. 05). Compared with the control group,the SOD and GSH-PX activities in the liver of the model group were decreased( P < 0. 05),and the MDA activity was increased( P < 0. 05). Compared with the model group,the activity of SOD and GSH-PX in liver tissue were increased in CDP low-dose group and CDP high-dose group( P < 0. 05),and MDA activity was decreased( P < 0. 05). Conclusion The CDP has anti-fatigue effect on aging mice induced by D-galactose. This effect may be related to the decrease of BUN and LA in serum,the increase of liver glycogen and muscle glycogen,and the enhancement of anti-free radical damage.
引文
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[3] Sun C,Fan S,Wang X,et al. Purple sweet potato color inhibits endothelial premature senescence by blocking the NLRP3 inflammasome[J]. Journal of Nutritional Biochemistry,2015,26(10):1029-1040.
[4]王建治,王长虹,周福波,等.姜黄对小鼠运动性疲劳的影响[J].医药导报,2009,28(12):1530-1532.
[5]农生斌,梁华益,黄明丽,等.百香果果汁对衰老小鼠负重游泳及抗疲劳的实验研究[J].现代预防医学,2017,44(7):1281-1284.
[6]宋昊翀,孙冉冉,张惠敏,等.衰老的中医理论研究[J].中华中医药杂志,2015,30(6):1889-1893.
[7]杨峻山.沙漠人参——肉苁蓉[J].中国药学杂志,2011,46(12):881.
[8]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2010:126.
[9]高晓霞,陈君,彭艳丽.肉苁蓉多糖药理作用研究概况[J].食品与药品,2015,17(2):136-139.
[10]许扬,吴涛,顾佳黎,等. D-半乳糖诱导衰老动物模型研究进展[J].中国老年学杂志,2009,29(13):1710-1713.
[11]王丽英,库宝善,姚海燕,等.七月七胶囊对负重游泳小鼠的抗疲劳作用[J].中国组织工程研究,2004,8(27):5940-5941.
[12]梁馨元,韦姗姗,李礼顺,等.芒柄花黄素对小鼠体内SOD、MDA、GSH-Px和BUN的影响[J].湖北科技学院学报(医学版),2017,31(3):188-190.
[13]陈松海,刘秋琼,麦颖娟,等.六味地黄汤对运动性疲劳小鼠的作用及血清乳酸、睾酮的影响[J].中药材,2011,34(11):1769-1770.
[14] Anand T,Phani KG,Pandareesh MD,et al. Effect of bacoside extract from Bacopa monniera on physical fatigue induced by forced swimming[J]. Phytotherapy Research,2012,26(4):587-593.
[15]陈志宏,齐聪儒,杨松鹤,等.器官衰老机理和自由基学说[J].承德医学院学报,2003,20(2):143-145.