黄酒对小鼠抗疲劳能力和衰老小鼠免疫器官的影响
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摘要
目的:研究黄酒对正常小鼠抗疲劳能力和D-半乳糖所致亚急性衰老小鼠免疫器官的影响。方法:抗疲劳能力实验中,小鼠分别灌胃给予双蒸水(0.25 m L/d)、高(0.4 m L/d)、中(0.25 m L/d)、低(0.15 m L/d)剂量的黄酒(乙醇体积分数为8%)15 d,小鼠游泳后检测肝糖原(hepatic glycogen,HG)、尿素氮(blood urea nitrogen,BUN)和血乳酸(blood lactic acid,BLA)含量的变化。在黄酒对亚急性衰老小鼠免疫器官影响的实验中,采用腹腔注射D-半乳糖建立亚急性衰老小鼠模型,分别灌胃双蒸水、酒精和不同剂量的黄酒,每天1次,连续灌胃56 d,测定小鼠胸腺指数、脾脏指数和亚急性衰老模型建立前后的体质量增值。结果:黄酒能显著提高小鼠体内HG的含量(P<0.01);增加运动后小鼠BLA增长率(P<0.05)和BUN的廓清率(P<0.05);黄酒能抑制衰老小鼠胸腺(P<0.01)和脾脏(P<0.05)质量的减少,有助于衰老小鼠的体质量增长(P<0.05)。结论:黄酒能提高小鼠的抗疲劳能力,增强衰老小鼠的免疫能力。
Objective: The aim of this study was to evaluate the anti-fatigue effect of Chinese rice wine(CRW) in normal mice and its influence on immune organ indexes of D-galactose-induced aging mice. Methods: Mice were randomly divided into four groups: control, low-dose CRW, middle-dose CRW and high-dose CRW groups, which were given by gavage 0.25 m L of distilled water, and 0.15, 0.25 and 0.4 m L of CRW for 15 consecutive days, respectively for exploring the antifatigue effect of CRW. Then, changes in hepatic glycogen, blood urea nitrogen(BUN) and blood lactic acid(BLA) contents in mice after forced swimming were measured. A mouse model of sub-acute aging was established by intraperitoneal injection of D-galactose and the aging mice were respectively given distilled water, aqueous ethanol and CRW once a day by intragastric administration for 56 days. Finally, the mice were sacrificed, for the measurement of thymus index, spleen index and body weight gain. Results: CRW could significantly increase hepatic glycogen level(P < 0.01) and enhance postexercise increase in BLA level(P < 0.05) and the clearance rate of BUN level(P < 0.05). In addition, CRW significantly inhibited the decrease in thymus(P < 0.01) and spleen(P < 0.05) weights and increased body weight(P < 0.05). Conclusion: CRW can exert an anti-fatigue effect and enhance immune function in aging mice.
Objective: The aim of this study was to evaluate the anti-fatigue effect of Chinese rice wine(CRW) in normal mice and its influence on immune organ indexes of D-galactose-induced aging mice. Methods: Mice were randomly divided into four groups: control, low-dose CRW, middle-dose CRW and high-dose CRW groups, which were given by gavage 0.25 m L of distilled water, and 0.15, 0.25 and 0.4 m L of CRW for 15 consecutive days, respectively for exploring the antifatigue effect of CRW. Then, changes in hepatic glycogen, blood urea nitrogen(BUN) and blood lactic acid(BLA) contents in mice after forced swimming were measured. A mouse model of sub-acute aging was established by intraperitoneal injection of D-galactose and the aging mice were respectively given distilled water, aqueous ethanol and CRW once a day by intragastric administration for 56 days. Finally, the mice were sacrificed, for the measurement of thymus index, spleen index and body weight gain. Results: CRW could significantly increase hepatic glycogen level(P < 0.01) and enhance postexercise increase in BLA level(P < 0.05) and the clearance rate of BUN level(P < 0.05). In addition, CRW significantly inhibited the decrease in thymus(P < 0.01) and spleen(P < 0.05) weights and increased body weight(P < 0.05). Conclusion: CRW can exert an anti-fatigue effect and enhance immune function in aging mice.
引文
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[2]王家林,张颖,于秦峰.黄酒中生物活性成分的探讨[J].酿酒科技,2014(7):47-50.
[3]黄玥,厉曙光,孙宇立,等.黄酒对D-半乳糖衰老小鼠抗氧化能力的影响[J].营养学报,2013,35(3):304-306.
[4]马良.黄酒对大鼠肠道微生物的影响[J].中华医院感染学杂志,2013(17):4107-4108;4124.
[5]倪赞,寿春波,史锋,等.黄酒对实验性铅中毒的拮抗作用研究[J].中国食品学报,2007,7(2):38-41.DOI:10.3969/j.issn.1009-7848.2007.02.008.
[6]谢广发,朱成钢,胡志明,等.绍兴黄酒对大鼠学习记忆力的影响[J].酿酒科技,2007(5):139-141.DOI:10.3969/j.issn.1001-9286.2007.05.036.
[7]沈赤,毛健,陈永泉,等.黄酒多糖对免疫缺陷小鼠血清免疫相关因子的影响[J].食品科学,2015,36(5):158-162.DOI:10.7506/spkx1002-6630-201505030.
[8]SHEN C,MAO J,CHEN Y Q,et al.Extraction optimization of polysaccharides from Chinese rice wine from the Shaoxing region and evaluation of its immunity activities[J].Journal of the Science of Food&Agriculture,2014,95(10):1991-1996.DOI:10.1002/jsfa.6909.
[9]白晨,王淑珍,周晓望,等.鹿茸血酒抗疲劳活性实验研究[J].食品科学,2008,29(11):575-578.DOI:10.3321/j.issn:1002-6630.2008.11.134.
[10]王亚东,王海玉,李立,等.五加皮酒抗疲劳作用的实验研究[J].中国卫生检验杂志,2009(6):1404-1405.
[11]刘敬科,赵巍,付会期,等.小米黄酒酿造工艺的研究[J].粮食与饲料工业,2009(12):22-23.
[12]ZHOU Y,DONG Y,XU Q G,et al.Mussel oligopeptides ameliorate cognition?deficit?and?attenuate?brain?senescence?in?D-galactose-induced aging mice[J].Food&Chemical Toxicology,2013,59(8):412-420.DOI:10.1016/j.fct.2013.06.009.
[13]王洪涛,尹花仙,金海珠,等.海参肽对小鼠抗疲劳作用的研究[J].食品与机械,2007,23(3):89-91.DOI:10.3969/j.issn.1003-5788.2007.03.025.
[14]郑哲君,李晓莉,王朔.抗疲劳功能食品的研究进展[J].食品科技,2006,31(2):4-7.DOI:10.3969/j.issn.1005-9989.2006.02.002.
[15]宋伟峰,苏俊芳,罗淑媛.荔枝多糖抗疲劳作用的实验研究[J].中药材,2012,35(9):1485-1487.
[16]NI W H,GAO T T,WANG H L,et al.Anti-fatigue activity of polysaccharides from the fruits of four Tibetan plateau indigenous medicinal plants[J].Journal of Ethnopharmacology,2013,150(2):529-535.DOI:10.1016/j.jep.2013.08.055.
[17]WANG J,LI S S,FAN Y Y,et al.Anti-fatigue activity of the watersoluble polysaccharides isolated from Panax ginseng C.A.Meyer.[J].Journal of Ethnopharmacology,2010,130(2):421-423.DOI:10.1016/j.jep.2010.05.027.
[18]王曦,许晓燕,郑林用,等.乳酸菌发酵黄芪的抗疲劳保健功能研究[J].时珍国医国药,2011,22(6):1422-1424.
[19]陈成,殷子建,徐速.浅析黄酒的历史及营养价值[J].酿酒,2002,29(1):55-56.DOI:10.3969/j.issn.1002-8110.2002.01.028.
[20]张睿,赵玉红,王忠政.鹿茸水提物对小鼠抗疲劳功能的影响[J].食品工业科技,2011,32(4):365-367.
[21]覃辉艳,赵鹏,杨俊峰,等.肉苁蓉茶对小鼠抗疲劳和耐缺氧能力的影响[J].现代预防医学,2011,38(12):2362-2364.
[22]任瑞琴,陈丹,程清,等.芙蓉李总多酚提取物对D-半乳糖所致亚急性衰老小鼠的抗氧化作用[J].中国现代应用药学,2014,31(1):5-9.
[23]FAN L P,DING S D,AI L Z,et al.Antitumor and immunomodulatory activity of water-soluble polysaccharide from Inonotus obliquus[J].Carbohydrate Polymers,2012,90(2):870-874.DOI:10.1016/j.carbpol.2012.06.013.
[24]WANG M,MENG X Y,YANG R L,et al.Cordyceps militaris polysaccharides can enhance the immunity and antioxidation activity in immunosuppressed mice[J].Carbohydrate Polymers,2012,89(2):461-466.DOI:10.1016/j.carbpol.2012.03.029.
[25]YANG T H,JIA M,ZHOU S Y,et al.Antivirus and immune enhancement activities of sulfated polysaccharide from Angelica sinensis.[J].International Journal of Biological Macromolecules,2012,50(3):768-772.DOI:10.1016/j.ijbiomac.2011.11.027.
[26]徐智,吴国明,钱桂生,等.大鼠衰老模型的初步建立[J].第三军医大学学报,2003,25(4):312-315.DOI:10.3321/j.issn:1000-5404.2003.04.010.
[27]余资江,应大君,董世武,等.半乳糖急性致衰老动物模型剂量的探讨[J].解剖学杂志,2005,28(4):422-424;445.DOI:10.3969/j.issn.1001-1633.2005.04.019.