食醋灌胃对小鼠扭体模型的镇痛作用
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摘要
目的:观察食醋灌胃疗法对小鼠扭体模型的镇痛作用。方法:24只昆明种小鼠随机分为三组:对照组、稀释食醋灌胃组和原醋灌胃组(n=8),给予各组小鼠灌胃相应药物(0.15ml/10g),每天1次,连续3天。于第3天给药30min后用使用醋酸扭体法测定各组小鼠扭体次数。结果:食醋灌胃疗法具有较强的镇痛作用,而且一定范围内存在剂量依赖性关系。与生理盐水组相比,稀释食醋灌胃组平均扭体次数减少43.7%,原食醋灌胃组平均扭体次数减少达85.0%,其中有50.0%的小鼠完全无扭体反应(P<0.01)。与稀释食醋灌胃组相比,原醋灌胃组扭体次数亦有统计学意义(P<0.01)。结论:食醋灌胃疗法对醋酸所致小鼠扭体模型有较强的镇痛作用。
Objective:To observe the analgesic effect of vinegar on writhing model in mice by intragastric administration. Methods:24 Kunming mice were randomly divided into 3 groups: control saline group, diluted vinegar group, vinegar groups(n=8 each).Each group of mice were givern corresponding drugs by intragastric administration, 0.15mL/10g, once a day for three days, then the times of body writhe were measured 30minites later by torsion body method. Results:Vinegar lavage therapy has strong analgesic effect and the drug also had time-dose dependency in a certain range. Compared with saline group,the average number of twisting of diluted vinegar group is reduced by 43.7% and the average number of twisting of vinegar group is reduced by 85.0%(P<0.01),50% of the mice having no writhing response. Compared with diluted vinegar group,the times of body writhe of vinegar group were also statistically significant(P<0.01). Conclusion:Inegar gavage therapy has strong analgesic effect on mice writhing model.
Objective:To observe the analgesic effect of vinegar on writhing model in mice by intragastric administration. Methods:24 Kunming mice were randomly divided into 3 groups: control saline group, diluted vinegar group, vinegar groups(n=8 each).Each group of mice were givern corresponding drugs by intragastric administration, 0.15mL/10g, once a day for three days, then the times of body writhe were measured 30minites later by torsion body method. Results:Vinegar lavage therapy has strong analgesic effect and the drug also had time-dose dependency in a certain range. Compared with saline group,the average number of twisting of diluted vinegar group is reduced by 43.7% and the average number of twisting of vinegar group is reduced by 85.0%(P<0.01),50% of the mice having no writhing response. Compared with diluted vinegar group,the times of body writhe of vinegar group were also statistically significant(P<0.01). Conclusion:Inegar gavage therapy has strong analgesic effect on mice writhing model.
引文
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[2]汪巍,陈映辉,王丽娜,等.柴胡与醋柴胡疏肝解郁作用比较研究[J].中成药,2014,03:617-619.
[3]信亚伟,孙惜时,谈甜甜,等.食醋的营养价值和保健功能作用研究进展[J].中国调味品,2015,02:124-127.
[4]刘珂.浅谈我国食醋的功能及发展趋势[J].中国调味品,2010,06:32-34+39.
[5]米醋浸脚治足跟痛法[J].健康生活,2008,05:14.
[6]巫朝霞,陶莉莉,陈小平,等.姜醋食疗防治产后病的临床研究[J].中外医疗,2014,04:16-18.
[7]戴体俊,喻田,麻醉药理学第三版[M].北京:人民卫生出版社,2015,102-103.
[8]刘和莉,李月玲,薛永志.不同温度和醋酸浓度对小鼠扭体疼痛模型的影响[J].包头医学院学报,2006,22(2):137-138.
[9]刘真,马晓丽,夏甜甜,孙海基.尼古丁对小鼠镇痛作用的研究[J].时珍国医国药,2013,02:279-280.
[10]胡洋洋,曲韵智,杜洪儒.活血止痛滴丸不同提取工艺的药效对比研究[J].黑龙江医药,2015,28(04):757-760.