不同干燥方式及清炒对荷叶中荷叶碱和槲皮素含量的影响
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摘要
目的:研究不同干燥方式及清炒对荷叶中荷叶碱和槲皮素含量的影响。方法:以荷叶碱和槲皮素含量为指标,对烘干、晒干、阴干及其清炒荷叶样品用高效液相色谱法进行测定,比较荷叶碱和槲皮素含量。结果:不同干燥方式及清炒后荷叶碱含量由高到低依次为:清炒烘干荷叶、清炒晒干荷叶、烘干荷叶、晒干荷叶、清炒阴干荷叶、阴干荷叶;槲皮素含量由高到低依次为:清炒烘干荷叶、清炒晒干荷叶、清炒阴干荷叶、烘干荷叶、晒干荷叶、阴干荷叶。烘干荷叶中的荷叶碱含量是晒干荷叶中含量的1. 23倍,是阴干荷叶中含量的2. 43倍,经清炒后烘干荷叶、晒干荷叶、阴干荷叶中的荷叶碱含量分别增加46. 70%、45. 84%、78. 83%;烘干荷叶与晒干荷叶中的槲皮素含量相近,是阴干荷叶中含量的2. 91倍,经清炒后烘干荷叶、晒干荷叶、阴干荷叶中的槲皮素含量分别增加876. 87%,862. 76%,701. 31%。结论:3种干燥方式及清炒对荷叶中荷叶碱和槲皮素含量有显著影响。
Objective: To study the effects of different drying methods and stir-frying on the contents of nuciferine and quercetin in lotus leaves. Methods: Taking the contents of nuciferine and quercetin as the indices,the contents of nuciferine and quercetin in dried,sun-dried,shade-dried and stir-fried samples of lotus leaves were determined by HPLC and the results were compared. Results: The content order of nuciferine in the samples with different drying methods and stir-frying was stir-fried dried lotus leaves > stir-fried sun-dried lotus leaves > dried lotus leaves > sun-dried lotus leaves > stir-fried shade-dried lotus leaves >shade-dried lotus leaves;the content order of quercetin was stir-fried dried lotus leaves > stir-fried sun-dried lotus leaves >stir-fried shade-dried lotus leaves > dried lotus leaves > sun-dried lotus leaves > shade-dried lotus leaves. The content of nuciferine in dried lotus leaves was 1. 23 times as that in sun-dried lotus leaves,and 2. 43 times as that in shade-dried lotus leaves. After stir-frying,the content of nuciferine in dried lotus leaves,sun-dried lotus leaves and shade-dried lotus leaves was increased by 46. 70%,45. 84% and 78. 83%,respectively;the content of quercetin in dried lotus leaves and sun-dried lotus leaves was similar,which was 2. 91 times as that in shade-dried lotus leaves. After stir-frying,the content of quercetin in dried lotus leaves,sun-dried lotus leaves and shade-dried lotus leaves was increased by 876. 87%,862. 76% and 701. 31%,respectively.Conclusion: Three drying methods and stir-frying have significant effects on the contents of nuciferine and quercetin in lotus leaves.
Objective: To study the effects of different drying methods and stir-frying on the contents of nuciferine and quercetin in lotus leaves. Methods: Taking the contents of nuciferine and quercetin as the indices,the contents of nuciferine and quercetin in dried,sun-dried,shade-dried and stir-fried samples of lotus leaves were determined by HPLC and the results were compared. Results: The content order of nuciferine in the samples with different drying methods and stir-frying was stir-fried dried lotus leaves > stir-fried sun-dried lotus leaves > dried lotus leaves > sun-dried lotus leaves > stir-fried shade-dried lotus leaves >shade-dried lotus leaves;the content order of quercetin was stir-fried dried lotus leaves > stir-fried sun-dried lotus leaves >stir-fried shade-dried lotus leaves > dried lotus leaves > sun-dried lotus leaves > shade-dried lotus leaves. The content of nuciferine in dried lotus leaves was 1. 23 times as that in sun-dried lotus leaves,and 2. 43 times as that in shade-dried lotus leaves. After stir-frying,the content of nuciferine in dried lotus leaves,sun-dried lotus leaves and shade-dried lotus leaves was increased by 46. 70%,45. 84% and 78. 83%,respectively;the content of quercetin in dried lotus leaves and sun-dried lotus leaves was similar,which was 2. 91 times as that in shade-dried lotus leaves. After stir-frying,the content of quercetin in dried lotus leaves,sun-dried lotus leaves and shade-dried lotus leaves was increased by 876. 87%,862. 76% and 701. 31%,respectively.Conclusion: Three drying methods and stir-frying have significant effects on the contents of nuciferine and quercetin in lotus leaves.
引文
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7李坤,王明芳,薛非非,等.荷叶炒炭过程中金丝桃苷、异槲皮苷与槲皮素含量变化规律研究[J].世界科学技术:中医药现代化,2016,18(1):112-117
2戴谆,宋金春,杨小青.荷叶碱的药理作用研究进展[J].中国药师,2016,19(5):988-991
3陈曦,戚进.荷叶中黄酮和生物碱的研究进展[J].中国实验方剂学杂志,2015,21(18):211-214
4张静怡,徐先顺,徐珽,等.HPLC法测定荷叶中荷叶碱[J].现代预防医学,2015,42(8):1479-1482
5赵艳波,施晓光.高效液相色谱法测定荷叶中总黄酮的含量[J].中国医院药学杂志,2010,30(20):1795-1796
6董春永,张学兰,李慧芬.炮制对荷叶中荷叶碱和槲皮素含量的影响[J].中成药,2010,32(6):973-976
7李坤,王明芳,薛非非,等.荷叶炒炭过程中金丝桃苷、异槲皮苷与槲皮素含量变化规律研究[J].世界科学技术:中医药现代化,2016,18(1):112-117