皱盖假芝子实体中1个新的降木脂素类化合物
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摘要
目的对皱盖假芝Amaurodermarude子实体的化学成分进行研究,以期发现新的活性化合物。方法运用硅胶、凝胶、MCI-gel树脂及RP-HPLC等多种色谱技术进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构。结果从皱盖假芝子实体中分离得到了3个化合物,分别鉴定为4-乙氧基-E-3-(3,4-二羟基苯亚甲基)-5-(3,4-二羟基苯基)呋喃-2-酮(1)、七叶内酯(2)、咖啡酸(3)。结论化合物1为新化合物,命名为皱盖假芝素A。
Objective For the purpose of finding new bioactive compounds from natural resources,the phytochemical investigation on the fruiting bodies of Amauroderma rude was carried out.Methods The chemical constituents from A.rude were isolated by various technologies,such as silica gel,Sephadex LH-20,MCI-gel resin,and high performance liquid chromatography.The isolated compounds were elucidated by spectroscopic methods,including extensive 1D,2D NMR,and ESI-MS techniques.Results Three compounds(1—3) including a new norlignan,were isolated from this mushroom.Their structures were identified as 4-ethyoyl-E-3-(3,4-dihydroxybenzylidene)-5-(3,4-dihydroxyphenyl)furan-2-one(1),esculetin(2),and caffeic acid(3).ConclusionCompound 1 is a new compound isolated from this fungus,which is identified as amauroderin A.
Objective For the purpose of finding new bioactive compounds from natural resources,the phytochemical investigation on the fruiting bodies of Amauroderma rude was carried out.Methods The chemical constituents from A.rude were isolated by various technologies,such as silica gel,Sephadex LH-20,MCI-gel resin,and high performance liquid chromatography.The isolated compounds were elucidated by spectroscopic methods,including extensive 1D,2D NMR,and ESI-MS techniques.Results Three compounds(1—3) including a new norlignan,were isolated from this mushroom.Their structures were identified as 4-ethyoyl-E-3-(3,4-dihydroxybenzylidene)-5-(3,4-dihydroxyphenyl)furan-2-one(1),esculetin(2),and caffeic acid(3).ConclusionCompound 1 is a new compound isolated from this fungus,which is identified as amauroderin A.
引文
[1]马海昇,李海珍,姚志伟.血芝液体培养及条件优化的初步研究[J].化学与生物工程,2010,27(3):70-72.
[2]吴兴亮,戴玉成.中国灵芝图鉴[M].北京:科学出版社,2005.
[3]Jiao C,Xie Y Z,Yang X,et al.Anticancer activity of Amauroderma rude[J].PLoS One,2013,8(6):66504-66517.
[4]李向敏.皱盖假芝和灵芝孢子中甾醇类化合物抗肿瘤作用机制研究[D].广州:华南理工大学,2015.
[5]夏险,刘健勇,涂俊铭.血芝深层液体培养研究初探[J].食用菌,2012,34(6):11-12.
[6]黄纪国,韩园园,谢意珍,等.响应面法优化超声波辅助提取皱盖假芝粗多糖工艺[J].食用菌学报,2015,22(2):62-67.
[7]杨丽梅.血芝的液体发酵及多糖提取工艺研究[D].武汉:湖北师范大学,2016.
[8]王谦,靳发彬,张俊刚,等.皱盖假芝深层发酵液多糖提取工艺优化研究[J].河北大学学报:自然科学版,2005,25(4):405-407.
[9]Bader A,De T N,Cotugno R,et al.Phenolic compounds from the roots of Jordanian viper's grass,Scorzonera judaica[J].J Nat Prods,2011,74(6):1421-1426.
[10]崔莹.寸节七化学成分研究[J].中草药,2011,42(8):1498-1501.
[11]Zhou L,Li D,Wang J,et al.Antibacterial phenolic compounds from the spines of Gleditsia sinensis Lam[J].Nat Prod Res,2007,21(4):283-291.
[2]吴兴亮,戴玉成.中国灵芝图鉴[M].北京:科学出版社,2005.
[3]Jiao C,Xie Y Z,Yang X,et al.Anticancer activity of Amauroderma rude[J].PLoS One,2013,8(6):66504-66517.
[4]李向敏.皱盖假芝和灵芝孢子中甾醇类化合物抗肿瘤作用机制研究[D].广州:华南理工大学,2015.
[5]夏险,刘健勇,涂俊铭.血芝深层液体培养研究初探[J].食用菌,2012,34(6):11-12.
[6]黄纪国,韩园园,谢意珍,等.响应面法优化超声波辅助提取皱盖假芝粗多糖工艺[J].食用菌学报,2015,22(2):62-67.
[7]杨丽梅.血芝的液体发酵及多糖提取工艺研究[D].武汉:湖北师范大学,2016.
[8]王谦,靳发彬,张俊刚,等.皱盖假芝深层发酵液多糖提取工艺优化研究[J].河北大学学报:自然科学版,2005,25(4):405-407.
[9]Bader A,De T N,Cotugno R,et al.Phenolic compounds from the roots of Jordanian viper's grass,Scorzonera judaica[J].J Nat Prods,2011,74(6):1421-1426.
[10]崔莹.寸节七化学成分研究[J].中草药,2011,42(8):1498-1501.
[11]Zhou L,Li D,Wang J,et al.Antibacterial phenolic compounds from the spines of Gleditsia sinensis Lam[J].Nat Prod Res,2007,21(4):283-291.