常州地区健康成人心肌酶谱干化学法参考区间调查
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摘要
目的建立常州地区健康成人天门冬氨酸氨基转移酶(AST)、血清乳酸脱氢酶(LDH)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)干化学法参考区间。方法通过随机抽样选取符合标准的1 305例健康成人作为研究对象,采集静脉血,采用美国强生公司VITROS 5.1FS干式化学分析仪检测血清AST、LDH、CK和CK-MB水平,并按年龄、性别进行分类统计。结果 AST参考区间:18~50岁男16~39 U/L,女13~34U/L;>50~79岁18~39U/L。LDH参考区间:18~60岁294~581U/L;>60~79岁345~638U/L。CK参考区间:18~79岁男54~189U/L,女40~171U/L。CK-MB参考区间:18~60岁男5~24U/L,女3~22U/L;>60~79岁10~24U/L。结论初步建立了常州地区健康成人心肌酶谱干化学法参考区间,为临床诊疗提供了相应的实验室参考依据。
Objective To establish the reference intervals of aspartate aminotransferase(AST),1 actate dehydrogenase(LDH),creatine kinase(CK)and creatine kinase MB(CK-MB)based on dry chemical system in healthy adults of Changzhou.Methods A total of 1 305 healthy adults who met the criteria were enrolled as the research object,and venous blood was collected.Serum AST,LDH,CK and CK MB levels were detected by VITROS 5.1 FS dry chemical analyzer of Johnson &Johnson Company of the United States,and classified according to age and gender.Results The reference interval of serum AST in 18-50 years old was 16-39 U/L(male),in 18-50 years old was 13-34 U/L(female),in>50-79 years old was 18-39 U/L.The reference intervals of LDH in 18-60 years old was 294-581 U/L,in>60-79 years old was 345-638.The reference intervals of CK in 18-79 years old was 54-189 U/L(male),in 18-79 years old was 40-171 U/L(female).The reference interval of CK-MB in 18-60 years old was 5-24 U/L(male),in 18-60 years old was 3-22 U/L(female),in>60-79 years old was 10-24 U/L.Conclusion The reference intervals of myocardial enzymes based on dry chemical system in healthy adults of Changzhou are preliminarily established,so as to provide basis for clinical diagnosis.
Objective To establish the reference intervals of aspartate aminotransferase(AST),1 actate dehydrogenase(LDH),creatine kinase(CK)and creatine kinase MB(CK-MB)based on dry chemical system in healthy adults of Changzhou.Methods A total of 1 305 healthy adults who met the criteria were enrolled as the research object,and venous blood was collected.Serum AST,LDH,CK and CK MB levels were detected by VITROS 5.1 FS dry chemical analyzer of Johnson &Johnson Company of the United States,and classified according to age and gender.Results The reference interval of serum AST in 18-50 years old was 16-39 U/L(male),in 18-50 years old was 13-34 U/L(female),in>50-79 years old was 18-39 U/L.The reference intervals of LDH in 18-60 years old was 294-581 U/L,in>60-79 years old was 345-638.The reference intervals of CK in 18-79 years old was 54-189 U/L(male),in 18-79 years old was 40-171 U/L(female).The reference interval of CK-MB in 18-60 years old was 5-24 U/L(male),in 18-60 years old was 3-22 U/L(female),in>60-79 years old was 10-24 U/L.Conclusion The reference intervals of myocardial enzymes based on dry chemical system in healthy adults of Changzhou are preliminarily established,so as to provide basis for clinical diagnosis.
引文
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[10]胡孝彬,逯心敏,于晓,等.健康人群心肌标志物参考区间调查[J].检验医学,2013,28(7):573-576.
[11]ZANINOTTO M,MION M M,NOVELLO E,et al.Creatine-kinase MB mass:age and sex-associated reference limits in two different platforms that use the same method[J].Clinica Chimica Acta,2009,401(1/2):162-164.
[12]ZHANG G M,XIA Y J,GUO X X,et al.Reference intervals of total bilirubin,ALT,AST,and creatinine in healthy elderly Chinese[J].Med Sci Monitor,2014,20(10):1778-1782.
[2]张广慧,邓琳,费爱华,等.增强型心肌肌钙蛋白I检测在急性冠状动脉综合征诊断中的应用[J].检验医学,2012,27(5):345-348.
[3] MOAMMAR M Q,ALI M I,MAHMOOD N A,et al.Cardiac troponinⅠlevels and alveolar-arterial oxygen gradient in patients with community-acquired pneumonia[J].Heart Lung Circ,2010,19(2):90-92.
[4]程玥,徐思琪,刘春华,等.杭州市健康人群血清中36项理化指标的参考区间调查[J].科技通报,2014,30(7):32-36.
[5] SHIMIZU Y,ICHIHARA K,ASIA-PACIFIC F B.Sources of variation analysis and derivation of reference intervals for ALP,LDH,and amylase isozymes using sera from the asian multicenter study on reference values[J].Clin Chim Acta,2015,446(6):64-72.
[6]曾洁,闫颖,张传宝,等.北京地区汉族人群ALT、AST、GGT和LDH的参考区间研究[J].中华检验医学杂志,2011,34(12):1073-1077.
[7] GEORGE M D,MCGILL N K,BAKER J F.Creatine kinase in the US population impact of demographics,comorbidities,and body composition on the normal range[J].Medicine,2016,95(33):33-36.
[8] STRUNZ C M,ARAKI L M,NOGUEURA A A,et al.Gender differences in serum CK-MB mass levels in healthy brazilian subjects[J].Braz J Med Biol Res,2011,44(3):236-239.
[9] APPLE F S,JESSE R L,NEWBY L K,et al.National academy of clinical biochemistry and IFCC committee for standardization of markers of cardiac damage laboratory medicine practice guidelines:analytical issues for biochemical markers of acute coronary syndromes[J].Clin Chem,2007,53(4):547-551.
[10]胡孝彬,逯心敏,于晓,等.健康人群心肌标志物参考区间调查[J].检验医学,2013,28(7):573-576.
[11]ZANINOTTO M,MION M M,NOVELLO E,et al.Creatine-kinase MB mass:age and sex-associated reference limits in two different platforms that use the same method[J].Clinica Chimica Acta,2009,401(1/2):162-164.
[12]ZHANG G M,XIA Y J,GUO X X,et al.Reference intervals of total bilirubin,ALT,AST,and creatinine in healthy elderly Chinese[J].Med Sci Monitor,2014,20(10):1778-1782.