多种外加碳源对厌氧氨氧化耦合反硝化的影响
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摘要
厌氧氨氧化反应功能菌世代时间长,生长条件苛刻,反应过程和脱氮效果在有机物存在的条件下极易受到影响。因此,本文在快速启动ANAMMOX反应的方法基础上,通过连续添加8种有机碳源进行批次试验,探究有机物ANAMMOX反应的影响。结果表明,不同有机物对厌氧氨氧化系统的促进作用不同,氨氧化速率从高到低依次为乙酸钠、丙酸钠、蔗糖、乳糖、淀粉、戊酸和己酸。当浓度达到300mg/L时,乙酸钠反硝化性能优于丙酸钠。以硝态氮为底物时,大分子有机物(戊酸和己酸)对提高反硝化脱氮性能帮助很小。
The anaerobic ammoxidation functional bacteria have a long generation,the growth conditions are harsh,and the reaction process and nitrogen removal efficiency are easy to be affected under the condition of the presence of organic matter.Therefore,based on the method of rapid start-up of ANAMMOX reaction,the effect of organic ANAMMOX reaction was investigated by batch test with 8 organic carbon sources.The results showed that the effects of different organic compounds on anammoxidation system were different.The order of ammonia oxidation rate was sodium acetate,sodium propionate,sucrose,lactose,starch,valproic acid and hexanoic acid from high to low.When the concentration reached 300 mg/l,the denitrification performance of sodium acetate was better than that of sodium propionate.When nitrate nitrogen is used as substrate,macromolecular organics(pentanoic acid and hexanoic acid)are of little help in improving denitrification performance.
The anaerobic ammoxidation functional bacteria have a long generation,the growth conditions are harsh,and the reaction process and nitrogen removal efficiency are easy to be affected under the condition of the presence of organic matter.Therefore,based on the method of rapid start-up of ANAMMOX reaction,the effect of organic ANAMMOX reaction was investigated by batch test with 8 organic carbon sources.The results showed that the effects of different organic compounds on anammoxidation system were different.The order of ammonia oxidation rate was sodium acetate,sodium propionate,sucrose,lactose,starch,valproic acid and hexanoic acid from high to low.When the concentration reached 300 mg/l,the denitrification performance of sodium acetate was better than that of sodium propionate.When nitrate nitrogen is used as substrate,macromolecular organics(pentanoic acid and hexanoic acid)are of little help in improving denitrification performance.
引文
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[7]喻徐良,汪瑶琪,陈重军,et al.ABR厌氧氨氧化反硝化协同脱氮除碳研究[J].工业安全与环保,2017,43(4):68-72.
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[9]刘金苓,钟玉鸣,谢志儒,et al.厌氧氨氧化微生物在有机碳源条件下的代谢特性[J].环境科学学报,2009,29(10):2041-2047.
[10]杨洋,左剑恶,沈平,等温度、pH值和有机物对厌氧氨氧化污泥活性的影响[J].环境科学,2006,27(4):691-695.
[2]废水生物处理新技术[M].2004.
[3]Kuypers M M,Lavik G,Woebken D,et al.Massive nitrogen loss from the Benguela upwelling system through anaerobic ammonium oxidation[J].Proc Natl Acad Sci U S A,2005,102(18):6478-6483.
[4]Phyllis L,Gaute L,Jensen M M,et al.Revising the nitrogen cycle in the Peruvian oxygen minimum zone[J].Proceedings of the National Academy of Sciences of the United States of America,2009,106(12):4752-4757.
[5]Ying Z,Xiao-Hong R,Camp H J M,Op Den,et al.Diversity and abundance of aerobic and anaerobic ammonium-oxidizing bacteria in freshwater sediments of the Xinyi River(China)[J].Environmental Microbiology,2010,9(9):2375-2382.
[6]汪瑶琪,喻徐良,陈重军,et al.有机物对厌氧氨氧化菌活性影响研究进展[J].化学通报,2017,80(2):173-178.
[7]喻徐良,汪瑶琪,陈重军,et al.ABR厌氧氨氧化反硝化协同脱氮除碳研究[J].工业安全与环保,2017,43(4):68-72.
[8]操沈彬,王淑莹,吴程程,杜睿,马斌,彭永臻.有机物对厌氧氨氧化系统的冲击影响[J].中国环境科学,2013,33(12):2164-2169.
[9]刘金苓,钟玉鸣,谢志儒,et al.厌氧氨氧化微生物在有机碳源条件下的代谢特性[J].环境科学学报,2009,29(10):2041-2047.
[10]杨洋,左剑恶,沈平,等温度、pH值和有机物对厌氧氨氧化污泥活性的影响[J].环境科学,2006,27(4):691-695.