基于市售侧柏炭饮片质量问题探讨其“炒炭存性”的质控标准
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摘要
目的:对市售侧柏炭质量进行考察,探讨侧柏叶"炒炭存性"的控制标准。方法:采用2015年版《中国药典》(一部)中方法对市售侧柏炭饮片的性状、水分、醇溶性浸出物含量进行检测,同时进行槲皮素的薄层色谱法(TLC)鉴别和有效成分槲皮苷、槲皮素的含量测定。结果:12批市售不同产地侧柏炭饮片中有9批炒炭程度较2015年版《中国药典》的标准重。建议对2015年版《中国药典》中TLC鉴别的展开条件进行修订,以避免展开剂分层;炒炭程度不同对侧柏炭所含槲皮苷和槲皮素含量的影响各异;同时检出槲皮苷和槲皮素可作为侧柏炭质量控制指标之一。结论:市售侧柏炭饮片大多存在炒炭程度过重的质量问题;建立的侧柏叶"炒炭存性"质控标准可用于有效保障侧柏炭饮片的质量。
Objective: To investigate the quality of Platycladi Cacumen carbonisata pieces in the market,then to explore control standard of "carbonizing retains characteristics"of Platycladi Cacumen. Method: The properties,water content and alcohol extract content of commercial Platycladi Cacumen carbonisata pieces were determined by the method in the 2015 edition of Chinese Pharmacopoeia. Thin layer chromatography( TLC)identification of quercetin and contents of quercetin and quercitin were determined. Result: The carbonizing degree of 9 batches of Platycladi Cacumen carbonisata pieces were heavier than which is specified in the 2015 edition of Chinese Pharmacopoeia. The conditions of TLC identification in the 2015 edition of Chinese Pharmacopoeia should be revised to avoid stratification; the carbonizing degree could affect the contents of quercetin and quercitin in Platycladi Cacumen carbonisata pieces; the simultaneous detection of quercetin and quercitin can be used as one of the quality control indexes of this carbonisata pieces. Conclusion: Most of commercial Platycladi Cacumen carbonisata pieces have the quality problem of excessive carbonizing degree,the established quality control standard of "carbonizing retains characteristics"can be used to effectively ensure the quality of Platycladi Cacumen carbonisata pieces.
Objective: To investigate the quality of Platycladi Cacumen carbonisata pieces in the market,then to explore control standard of "carbonizing retains characteristics"of Platycladi Cacumen. Method: The properties,water content and alcohol extract content of commercial Platycladi Cacumen carbonisata pieces were determined by the method in the 2015 edition of Chinese Pharmacopoeia. Thin layer chromatography( TLC)identification of quercetin and contents of quercetin and quercitin were determined. Result: The carbonizing degree of 9 batches of Platycladi Cacumen carbonisata pieces were heavier than which is specified in the 2015 edition of Chinese Pharmacopoeia. The conditions of TLC identification in the 2015 edition of Chinese Pharmacopoeia should be revised to avoid stratification; the carbonizing degree could affect the contents of quercetin and quercitin in Platycladi Cacumen carbonisata pieces; the simultaneous detection of quercetin and quercitin can be used as one of the quality control indexes of this carbonisata pieces. Conclusion: Most of commercial Platycladi Cacumen carbonisata pieces have the quality problem of excessive carbonizing degree,the established quality control standard of "carbonizing retains characteristics"can be used to effectively ensure the quality of Platycladi Cacumen carbonisata pieces.
引文
[1]孙立靖,谢英渤.色质联用分析炮制工艺对侧柏叶挥发油的影响[J].山东师范大学学报:自然科学版,2001,16(3):357-360.
[2]罗世恒,陈世忠,王月茹,等. HPLC法测定侧柏叶中杨梅苷的含量[J].陕西中医学院学报,2010,33(3):85-86.
[3]陈学松,张涛.侧柏叶不同炮制品中槲皮苷含量的比较[J].中国中药杂志,2006,31(10):847-849.
[4]曾祥丽,丁安伟,单鸣秋.侧柏叶炮制前后鞣质的含量测定[J].中国中医药信息杂志,2008,15(4):45-46.
[5]吴怀恩,甄汉深,韦志英,等. RP-HPLC法同时测定侧柏叶炭中槲皮素和山柰素的含量[J].中国药房,2009,20(12):934-935.
[6]江波,孙立立,杨书斌,等. HPLC测定侧柏炭中槲皮苷和槲皮素的含量[J].中成药,2005,27(7):792-794.
[7]谭晓亮,李瑞海,贾天柱.侧柏叶炮制前后成分的对比[J].实用药物与临床,2015,18(11):1359-1362.
[8]万军,周霞,吴纯洁.中国药典中的炭药质量标准浅析[J].中国医院药学杂志,2007,27(11):1635-1636.
[9]黄樱华,黄月纯,魏刚,等.正交试验法筛选侧柏叶总黄酮的提取工艺[J].中国实验方剂学杂志,2009,15(11):34-37.
[10]孙立立,杨书斌,江波,等.炮制对侧柏叶化学成分的影响[J].中成药,2006,28(6):821-823.
[2]罗世恒,陈世忠,王月茹,等. HPLC法测定侧柏叶中杨梅苷的含量[J].陕西中医学院学报,2010,33(3):85-86.
[3]陈学松,张涛.侧柏叶不同炮制品中槲皮苷含量的比较[J].中国中药杂志,2006,31(10):847-849.
[4]曾祥丽,丁安伟,单鸣秋.侧柏叶炮制前后鞣质的含量测定[J].中国中医药信息杂志,2008,15(4):45-46.
[5]吴怀恩,甄汉深,韦志英,等. RP-HPLC法同时测定侧柏叶炭中槲皮素和山柰素的含量[J].中国药房,2009,20(12):934-935.
[6]江波,孙立立,杨书斌,等. HPLC测定侧柏炭中槲皮苷和槲皮素的含量[J].中成药,2005,27(7):792-794.
[7]谭晓亮,李瑞海,贾天柱.侧柏叶炮制前后成分的对比[J].实用药物与临床,2015,18(11):1359-1362.
[8]万军,周霞,吴纯洁.中国药典中的炭药质量标准浅析[J].中国医院药学杂志,2007,27(11):1635-1636.
[9]黄樱华,黄月纯,魏刚,等.正交试验法筛选侧柏叶总黄酮的提取工艺[J].中国实验方剂学杂志,2009,15(11):34-37.
[10]孙立立,杨书斌,江波,等.炮制对侧柏叶化学成分的影响[J].中成药,2006,28(6):821-823.