天然低共熔溶剂提取油茶蒲中植物多酚的研究
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摘要
以油茶蒲为原料,对照传统的水和60%乙醇溶液,选择几种天然低共熔溶剂提取油茶蒲中的植物多酚,考察了天然低共熔溶剂对多酚得率的影响。通过单因素试验和响应面试验对天然低共熔溶剂提取油茶蒲中多酚的工艺条件进行了研究和优化。结果发现:天然低共熔溶剂具有比传统溶剂更好的提取效果;油茶蒲中多酚提取的最优条件为提取溶剂柠檬酸-氯化胆碱(摩尔比1∶3)、提取时间37 min、提取温度81℃、液料比42∶1,在最优条件下多酚得率为0. 932 1%。
Several natural deep eutectic solvents were selected as extraction solvents to extract plant polyphenol form Camellia oleifera fruit hull,and the poloyphenol yields of natural eutectic solvents extractions were compared with those by traditional water and 60% ethanol solution.The conditions of extracting polyphenol from Camellia oleifera fruit hull with natural deep eutectic solvent were studied and optimized by single factor experiments and response surface methodology.The results showed that the natural eutectic solvent had better effects than traditional solvent.The optimal extraction conditions were obtained as follows:citric acid-choline chloride(molar ratio 1 ∶ 3) used as extraction solvent,extraction time 37 min,extraction temperature 81 ℃,ratio of liquid to material 42 ∶ 1.Under the optimal conditions,the polyphenol yield was 0.932 1%.
Several natural deep eutectic solvents were selected as extraction solvents to extract plant polyphenol form Camellia oleifera fruit hull,and the poloyphenol yields of natural eutectic solvents extractions were compared with those by traditional water and 60% ethanol solution.The conditions of extracting polyphenol from Camellia oleifera fruit hull with natural deep eutectic solvent were studied and optimized by single factor experiments and response surface methodology.The results showed that the natural eutectic solvent had better effects than traditional solvent.The optimal extraction conditions were obtained as follows:citric acid-choline chloride(molar ratio 1 ∶ 3) used as extraction solvent,extraction time 37 min,extraction temperature 81 ℃,ratio of liquid to material 42 ∶ 1.Under the optimal conditions,the polyphenol yield was 0.932 1%.
引文
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[2]李利敏,沈建福,吴晓琴. 8种油茶蒲提取物中活性物质含量及其抗氧化能力的比较研究[J].中国粮油学报,2013,28(1):41-47.
[3]张力平,张长霞,李俊清,等.植物多酚的研究现状及其发展前景[J].林业科学,2005,41(6):157-162.
[4]冯丽,宋曙辉,赵霖,等.植物多酚种类及其生理功能的研究进展[J].江西农业学报,2007,19(10):105-107.
[5]初乐,赵岩,和法涛,等.植物多酚提取与检测方法的研究进展[J].中国果菜,2014,34(4):62-65.
[6]王贤萍,韩廖龙,段泽敏.植物多酚类物质的络合萃取[J].山西农业科学,2014,42(3):285-288.
[7]ABBOTT A P,BOOTHBY D,CAPPER G,et al. Deep eutectic solvents formed between choline chloride and carboxylic acids:versatile alternatives to ionic liquids[J]. J Am Chem Soc,2004,126(29):9142-9147.
[8]DAI Y T,WITKAMP G J,VERPOORTE R,et al. Tailoring properties of natural deep eutectic solvents with water to facilitate their applications[J]. Food Chem,2015,187:14-19.
[9] CHOI Y H,VAN SPRONSEN J,DAI Y T,et al. Are natural deep eutectic solvents the missing link in understanding cellular metabolism and physiology?[J]. Plant Physiol,2011,156(4):1701-1705.
[10]DAI Y T,VAN SPRONSEN J,WITKAMP G J,et al.Natural deep eutectic solvents as new potential media for green technology[J]. Anal Chim Acta,2013,766:61-68.
[11]WEI Z F,QI X L,LI T T,et al. Application of natural deep eutectic solvents for extraction and determination of phenolics in Cajanus cajan leaves by ultra performance liquid chromatography[J]. Sep Purif Technol,2015,149:237-244.
[12]WEI Z F,WANG X Q,PENG X,et al. Fast and green extraction and separation of main bioactive flavonoids from Radix Scutellariae[J]. Ind Crops Prod,2015,63:175-181.
[13]NAM M W,ZHAO J,LEE M S,et al. Enhanced extraction of bioactive natural products using tailor-made deep eutectic solvents:application to flavonoid extraction from Flos sophorae[J]. Green Chem,2015,17(3):1718-1727.
[14]RADOSEVIC K,CURKO N,SRCEK V G,et al. Natural deep eutectic solvents as beneficial extractants for enhancement of plant extracts bioactivity[J]. LWT-Food Sci Technol,2016,73:45-51.
[15]GARCA A,RODRIGUEZ-JUAN E,RODRIGUEZ-GUTIERREZ G,et al. Extraction of phenolic compounds from virgin olive oil by deep eutectic solvents(DESs)[J].Food Chem,2016,197:554-561.
[16]林玉芳,陈清西,关夏玉,等.橄榄总多酚提取工艺优化研究[J].中国农学通报,2011,27(5):396-400.
[17]王翼,包益桂.合欢花中多酚提取工艺的研究[J].黑龙江畜牧兽医,2015(8):214-216.
[18]马永强,袁诺,葛林,等.甜玉米芯多酚提取工艺研究[J].农产品加工,2015(12):36-38.
[19]孙建.荷叶中多酚类物质的提取、分离及抗氧化活性研究[D].安徽芜湖:安徽工程大学,2016.
[20]姚永志,王子涵,左锦静.乙醇提取花生红衣多酚物质的研究[J].现代食品科技,2006,32(3):51-53.
[21]朱登祥,尉连玲,侯志敏.葡萄籽中多酚类物质提取工艺研究[J].安徽农业科学,2008,36(17):7068-7070.
[22]郭丽萍,朱英莲.香蕉皮中多酚物质的提取[J].食品与药品,2008,10(11):32-34.
[23]李宏.苹果皮渣中苹果多酚的提取工艺优化及其抗脂质氧化活性研究[J].赤峰学院学报,2008,24(5):40-42.