鼠李糖脂、Fe~(3+)及Mg~(2+)对蜡质芽孢杆菌DL-1降解邻二氯苯的复合影响
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摘要
以蜡质芽孢杆菌DL-1降解邻二氯苯,研究不同浓度的鼠李糖脂、Fe~(3+)、Mg~(2+)对邻二氯苯生物降解效果的影响。通过响应面优化法获得最适加入量,并考察添加鼠李糖脂、Fe~(3+)、Mg~(2+)的生物滴滤塔中邻二氯苯废气的处理效果。实验结果表明:鼠李糖脂、Fe~(3+)、Mg~(2+)的最适加入量分别为120,4.0,2.0 mg/L;在最适加入量下培养48 h后,邻二氯苯降解菌DL-1能够保持菌体完整,培养72 h后邻二氯苯去除率可达98.3%;在鼠李糖脂、Fe~(3+)、Mg~(2+)加入量为最适加入量、空床停留时间为60 s、进气中邻二氯苯质量浓度为1 000 mg/m3时,生物滴滤塔对邻二氯苯的去除率达到90.0%。
o-Dichlorobenzeneas was degraded by a strain of Bacillus cereus DL-1.The effects of rhamnolipid,Fe~(3+)and Mg~(2+) with different concentration on the bio-degradation were studied.Their optimum conditions were obtained by using response surface optimization method,and the treatment effect of o-dichlorobenzene-containing waste gas in a biotrickling filter with rhamnolipid,Fe~(3+) and Mg~(2+) addition were investigated.The experimental results show that:The optimum dosage of rhamnolipid,Fe~(3+),and Mg~(2+) is 120,4.0,2.0 mg/L respectively;Under the optimum dosages,the Bacillus cereus DL-1 strain can keep intact after cultured for 48 h,and the o-dichlorobenzene removal rate is 98.3%after cultured for 72 h;When rhamnolipid,Fe~(3+),and Mg~(2+) are added with the optimum dosage,the empty bed residence time is 60 s and the mass concentration of o-dichlorobenzene in the biotrickling filter inlet is 1 000 mg/m~3,the o-dichlorobenzene removal rate is 90.0%.
o-Dichlorobenzeneas was degraded by a strain of Bacillus cereus DL-1.The effects of rhamnolipid,Fe~(3+)and Mg~(2+) with different concentration on the bio-degradation were studied.Their optimum conditions were obtained by using response surface optimization method,and the treatment effect of o-dichlorobenzene-containing waste gas in a biotrickling filter with rhamnolipid,Fe~(3+) and Mg~(2+) addition were investigated.The experimental results show that:The optimum dosage of rhamnolipid,Fe~(3+),and Mg~(2+) is 120,4.0,2.0 mg/L respectively;Under the optimum dosages,the Bacillus cereus DL-1 strain can keep intact after cultured for 48 h,and the o-dichlorobenzene removal rate is 98.3%after cultured for 72 h;When rhamnolipid,Fe~(3+),and Mg~(2+) are added with the optimum dosage,the empty bed residence time is 60 s and the mass concentration of o-dichlorobenzene in the biotrickling filter inlet is 1 000 mg/m~3,the o-dichlorobenzene removal rate is 90.0%.
引文
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[2]Mc Loughlin E,Rhodes A H,Owen S M,et al.Biogenic volatile organic compounds as a potential stimulator for organic contaminant degradation by soil microorganisms[J].Environ Pollut,2009,157(1):86-94.
[3]Peng Xianjia,Li Yanhui,Luan Zhaokun,et al.Adsorption of 1,2-dichlorobenzene from water to carbon nanotubes[J].Chem Phys Lett,2003,376(1):154-158.
[4]Zhu Runye,Kennes C,Cheng Zhuowei,et al.Styrene removal in a biotrickling filter and a combined UV-biotrickling filter:Steady and transient-state performance and microbial analysis[J].Chem Eng J,2015,275:168-178.
[5]徐校良,王志良,吴海锁,等.生物滴滤塔处理甲苯废气[J].化工环保,2013,33(3):193-197.
[6]Zhang Zhixiang,Jiang Zheng,Shangguan Wenfeng.Low-temperature catalysis for VOCs removal in technology and application:A state-of-the-art review[J].Catal Today,2016,264:270-278.
[7]姜萍萍,郭楚玲,党志,等.鼠李糖脂与疏水底物及其降解菌的相互作用[J].环境科学,2011,7(32):2144-2150.
[8]孙红启.铁载体和铁离子对细菌生长过程的影响[D].济南:山东大学,2008.
[9]谢治民,李建华,王海洋,等.Fe-厌氧微生物体系降解2,4,6-三氯酚[J].化工环保,2009,29(02):105-108.
[10]王璐.表面活性剂Tween-20及Zn(Ⅱ)强化生物滴滤器处理有机废气的性能研究[D].长沙:湖南大学,2014.
[11]Wang Lu,Yang Chunping,Cheng Yan,et al.Effects of surfactant and Zn(Ⅱ)at various concentrations on microbial activity and ethylbenzene removal in biotricking filter[J].Chemosphere,2013,93:2909-2913.
[12]Su Chengyuan,Li Weiguang,Chen Menglin,et al.Effect of iron-manganese-sepiolite as heterogeneous Fenton-like catalyst on the performance and microbial community of anaerobic granular sludge treatment system[J].Bioresour Technol,2016,200:1065-1072.
[13]陈延君,王红旗,王然,等.鼠李糖脂对微生物降解正十六烷以及细胞表面性质的影响[J].环境科学,2007,32(9):2144-2150.
[14]Liu Chunjing,Liu Jia,Li Jian,et al.Removal of H2S by co-immobilized bacteria and fungi biocatalysts in a bio-trickling filter[J].Process Saf Environ Prot,2013,91:145-152.
[15]杨百忍,王丽萍,牛仙,等.生物滴滤塔处理氯苯废气的工艺性能[J].化工环保,2014,34(3):201-205.
[16]Zhang Jingying,Li Lin,Liu Junxin.Thermophilic biofilter for SO2 removal:Performance and microbial characteristics[J].Bioresour Technol,2015,180:106-111.
[17]刘慧慧,杨春生,丁成.一株1,2-二氯苯降解菌的分离鉴定及其降解特性[J].环境工程学报,2011,9(5):2151-2155.
[18]中国环境保护部.HJ 621-2011水质氯苯类化合物的测定[S].北京:中国环境科学出版社,2011.
[19]黄廷林,周娜,张海涵,等.3株贫营养好氧反硝化细菌的分离鉴定及反硝化特性[J].环境工程学报,2014,8(12):5507-5513.
[20]Zhao Z,Selvam A,Wong W C.Effects of rhamnolipids on cell surface hydrophobicity of PAH degrading bacteria and the biodegradation of phenanthrene[J].Bioresour Technol,2012,111:328-335.