基于细胞氧化应激模型的南瓜多糖与人参皂苷联合抗氧化作用研究
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摘要
研究在南瓜多糖与人参皂苷联合作用下对过氧化氢损伤后的小鼠肝细胞的抗氧化作用。取小鼠活体肝细胞进行H_2O_2诱导,成功建立细胞氧化应激损伤模型。将细胞分为正常组、模型组、不同浓度下的南瓜多糖组、人参皂苷组及联合组,考察各组分在细胞内的抗氧化活性。试验结果表明,经分离纯化的南瓜多糖具有抗氧化活性,南瓜多糖与人参皂苷Rg1联合使用时,细胞活力明显增强,在丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)及过氧化氢酶(catalase,CAT)指标上具有联合增效作用,抗氧化效果更加明显,缓解了氧化损伤带来的影响。
The antioxidant effect of pumpkin polysaccharide and ginsenoside on the liver cells of mice after hydrogen peroxide injury was studied. In vivo mouse hepatocytes were taken for H_2 O_2 induction,and the cell oxidative stress injury model was successfully established. The cells were divided into normal group,model group,pumpkin polysaccharide group at different concentrations,ginsenoside group and combination group to investigate the antioxidant activities of each component in the cells. Experimental results showed that the separation and purification of pumpkin polysaccharide had antioxidant activity,pumpkin polysaccharide and ginseng saponin Rg1 joint, when using cell vitality enhanced obviously, than a single component in malondialdehyde(MDA),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD)and catalase(CAT)index on the joint synergy,antioxidant effect was more obvious,and it mitigated the effects of oxidative damage.
The antioxidant effect of pumpkin polysaccharide and ginsenoside on the liver cells of mice after hydrogen peroxide injury was studied. In vivo mouse hepatocytes were taken for H_2 O_2 induction,and the cell oxidative stress injury model was successfully established. The cells were divided into normal group,model group,pumpkin polysaccharide group at different concentrations,ginsenoside group and combination group to investigate the antioxidant activities of each component in the cells. Experimental results showed that the separation and purification of pumpkin polysaccharide had antioxidant activity,pumpkin polysaccharide and ginseng saponin Rg1 joint, when using cell vitality enhanced obviously, than a single component in malondialdehyde(MDA),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD)and catalase(CAT)index on the joint synergy,antioxidant effect was more obvious,and it mitigated the effects of oxidative damage.
引文
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[10]段小群,焦杨,张士军,等.玉郎伞多糖对过氧化氢诱导大鼠原代肝细胞损伤的保护作用[J].时珍国医国药,2007(7):1592-1593
[11]Han D,Matsumaru K,Rettori D,et al.Usnic acid-induced necrosisof cultured mouse hepatocytes:inhibition of mitochondrial function andoxidative stress[J].Biochemical Pharmacology,2004,67(3):439-440
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[13]Lee HS,Won NH,Kim KH,et al.Antioxidant effects of aqueous extract of Terminalia chebula in vivo and in vitro[J].Biological and Pharmaceutical Bulletin,2005,28(9):1639-1640
[2]苏丹.红外干燥对南瓜品质的影响研究[C]//中国机械工程学会包装与食品工程分会2010年学术年会论文集.上海:中国机械工程学会包装与食品工程分会,2010:6
[3]Chen X,Zhang J,Fang Y et al.Ginsenoside Rg1 delays tert-butyl hy droperoxide-induced premature senescence in human WI-38diploid fibroblast cells[J].Gerontol A Biol Sci Med Sci,2008;63(3):253-264
[4]Sun J,Song X,Hu S.Ginsenoside Rg1 and aluminum hydroxide synergistically promote immune responses to ovalbumin in BALB/c mice[J].ClinVaccine Immunol,2008;15(2):303-307
[5]刘紫萱.三种植物活性成分联合改善2型糖尿病小鼠氧化应激效果及机制研究[D].天津:天津农学院,2017:33-35
[6]SAW C L L,YANG A Y Q,CHENG D C,et al.Pharmacodynamics of Ginsenosides:Antioxidant Activities,Activation of Nrf2,and Potential Synergistic Effects of Combinations[J].Chem Res Toxicol,2012,25(8):1574-1580.
[7]齐晓龙,赵芹,张亚男,等.过氧化氢诱导产蛋鸡原代肝细胞氧化应激模型的建立[J].中国畜牧杂志,2014,49(11):49-52
[8]ZHAO X C,ZHANG L,YU H X,et al.Curcumin protects mouse neuroblastoma Neuro-2A cells against hydrogen-peroxide-inducedoxidativestress[J].Food Chemistry,2011,129(2):387-394
[9]李凤玉,贾胜男,马爽,等.人参皂苷Rg1抑制C2C12细胞MuRF-1/Atrogin-1表达研究[J].长春师范大学学报,2017,36(2):65-70
[10]段小群,焦杨,张士军,等.玉郎伞多糖对过氧化氢诱导大鼠原代肝细胞损伤的保护作用[J].时珍国医国药,2007(7):1592-1593
[11]Han D,Matsumaru K,Rettori D,et al.Usnic acid-induced necrosisof cultured mouse hepatocytes:inhibition of mitochondrial function andoxidative stress[J].Biochemical Pharmacology,2004,67(3):439-440
[12]Shishido S,Koga H,Harada M,et al.Hydrogen peroxide overproduction in megamitochondria of troglitazone-treated human hepatocytes[J].Hepatology,2003,37(1):136-138
[13]Lee HS,Won NH,Kim KH,et al.Antioxidant effects of aqueous extract of Terminalia chebula in vivo and in vitro[J].Biological and Pharmaceutical Bulletin,2005,28(9):1639-1640