绿山楂种子内源抑制物成分的初步鉴定
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摘要
为研究绿山楂种子的休眠机制,采用系统溶剂分离法,分离出绿山楂种子的石油醚相、乙醚相、乙酸乙酯相、甲醇相和水相,并利用白菜籽发芽试验对这5个相进行生物活性测定,同时结合气相色谱-质谱(GC-MS)联用仪分析造成绿山楂种子休眠的内源抑制物的成分。结果表明:1)绿山楂种子各萃取相的溶液对白菜种子发芽均有一定的抑制作用,其中抑制作用最强的是乙醚相,其次是甲醇相;2)通过GC-MS联用仪进一步的分离鉴定发现,绿山楂种子中存在的内源抑制物主要以酚类、酯类、有机酸类等为主,同时还包括数种未知物质。
To know the dormancy mechanism of the C.viridis seeds clearly,the method of system solvent separation was used to separate the petroleum ether phase,the ether phase,the ethyl acetate phase,the methanol phase and the water phase of the seeds of the C.viridis.Also the germination of non-dormant cabbage seeds were used to determine the biological activity of these five phases.The components of endogenous inhibitors that caused the dormancy of C.viridis seeds were analyzed by GC-MS.The results showed:1)The solution of extraction phase of the C.viridis seeds had some inhibitory effects on the germination of cabbage seeds.Among them,the strongest inhibitory effect was found in the ether phase,followed by the methanol phase.2)Further separation and identification by GC-MS showed that the endogenous inhibitors presented in C.viridis seeds were mainly composed of phenols,esters,organic acids etc,as well as several unknown substances.
To know the dormancy mechanism of the C.viridis seeds clearly,the method of system solvent separation was used to separate the petroleum ether phase,the ether phase,the ethyl acetate phase,the methanol phase and the water phase of the seeds of the C.viridis.Also the germination of non-dormant cabbage seeds were used to determine the biological activity of these five phases.The components of endogenous inhibitors that caused the dormancy of C.viridis seeds were analyzed by GC-MS.The results showed:1)The solution of extraction phase of the C.viridis seeds had some inhibitory effects on the germination of cabbage seeds.Among them,the strongest inhibitory effect was found in the ether phase,followed by the methanol phase.2)Further separation and identification by GC-MS showed that the endogenous inhibitors presented in C.viridis seeds were mainly composed of phenols,esters,organic acids etc,as well as several unknown substances.
引文
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[23]韩宝瑞,徐凌志.西洋参果实中发芽抑制物质研究[J].安徽农业科学,2010,38(14):7 302-7 304.
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[25]郭廷翘,李暐,郭维明,等.水曲柳种子主要天然发芽抑制物的鉴定及生物化学研究[J].东北林业大学学报,1991,19:358-365.
[26]吴易,司春灿,孙见凡,等.南美蟛蜞菊根系分泌物中2种化学物质的化感潜力[J].西北农业学报,201,23(10):170-178.
[27]杨晓玲,张培玉,郭明军.山楂种子酚类物质含量与休眠的关系[J].园艺学报,1997,24(4):393-394.
[28]何芳,张宁.酚类物质对种子在解除休眠过程中的作用[J].建筑工程技术与设计,2015(9):2 891.
[2]刘雯.浅谈山楂栽培技术及经济价值[J].农业与技术,2015,35(19):93-94.
[3]孔祥海.抑制物质与种子休眠[J].龙岩师专学报,2002,20(6):50-52.
[4]郭江帆,孙佳,魏朔南.秦艽种子内源萌发抑制物的初步研究[J].种子,2013,32(7):39-44.
[5]张艳杰,高捍东,鲁顺保.南方红豆杉种子中发芽抑制物的研究[J].南京林业大学学报(自然科学版),2007,31(4):51-56.
[6]王小平,王九龄.白皮松种子内含物的提取分离及生物测定[J].种子,1998,17(5):19-22,28.
[7]赵敏,王炎,康利.刺五加果实及种子内源萌发抑制物质活性的研究[J].中国中药杂志,1998,26(1):159-168.
[8]杨晓玲,郭守华,张建文,等.山楂种子抑制物质的提取分离及生物测定[J].经济林研究,2008,26(4):63-67.
[9]杨晓玲,郭守华,关学敏,等.山楂种子层积过程中抑制物质的变化[J].经济林研究,2009,27(2):44-48.
[10]朱梅玲,乔进春.花前花期管理对山楂生长发育的影响[J].经济林研究,1996,14(2):22.
[11]苗卫东,扈惠灵,周瑞金,等.野生山楂种子休眠特性及破除方法探讨[J].北方园艺,2011(7):27-29.
[12]程荣臣.山楂单性结实现象及诱导方法的研究[J].经济林研究,1996,14(3):11-13.
[13]何钢,吴若炎,赵思东,等.山楂主要品种果实品质分析比较[J].经济林研究,1996,14(4):36-37.
[14]张培玉,杨晓玲.山楂种子休眠,萌发与内源激素含量的变化[J].河北科技师范学院学报,1999(1):7-10.
[15]李秉真,乌云,田瑞华,等.山楂种子休眠与后熟期间内源激素的变化[J].植物生理学通讯,1998,34(4):254-256.
[16]ISTA(2013).International Rules for Seed Testing.The International Seed Testing Association,Zurich,Switzerland.Chapter,2013,10:2-3.
[17]张艳杰.南方红豆杉种子休眠机理的研究[D].南京林业大学,2007.
[18]黄耀阁,崔树玉,鲁歧,等.西洋参种子抑制物质的初步研究[J].吉林农业大学学报,1994,16(2):9-14.
[19]中华人民共和国国家标准GB2772-1999,《林木种子检验规程》,国家质量监督局.
[20]Riis P,Meiling E,Peetz J.Determination of germination percentage and germination index-collaborative trial and ruggedness testing[J].Journal of the Institute of Brewing,1995,101(3):171-173.
[21]韩宝瑞,黄耀阁,李向高,等.西洋参果肉中的六种发芽抑制物质[J].特产研究,2000(1):13-17.
[22]高向阳,韩帅,马荣坤.郑州地区野生贼小豆资源特性研究初报[J].广东农业科学,2013,40(12):164-166.
[23]韩宝瑞,徐凌志.西洋参果实中发芽抑制物质研究[J].安徽农业科学,2010,38(14):7 302-7 304.
[24]孙晓刚,丁言,郭太君.凤丹胚乳内源抑制物质活性及其成分的GC-MS鉴定[J].西北农林科技大学学报(自然科学版),2016(5).
[25]郭廷翘,李暐,郭维明,等.水曲柳种子主要天然发芽抑制物的鉴定及生物化学研究[J].东北林业大学学报,1991,19:358-365.
[26]吴易,司春灿,孙见凡,等.南美蟛蜞菊根系分泌物中2种化学物质的化感潜力[J].西北农业学报,201,23(10):170-178.
[27]杨晓玲,张培玉,郭明军.山楂种子酚类物质含量与休眠的关系[J].园艺学报,1997,24(4):393-394.
[28]何芳,张宁.酚类物质对种子在解除休眠过程中的作用[J].建筑工程技术与设计,2015(9):2 891.