BAG-1和NF-κB在结直肠癌中的表达及临床意义
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摘要
目的探讨BAG-1、NF-κB在结直肠癌中的表达情况及其临床意义。方法利用免疫组织化学染色方法检测80例结直肠腺癌、40例结直肠腺瘤及40例癌旁正常结直肠黏膜组织中BAG-1与NF-κB蛋白的表达情况,分析BAG-1及NF-κB的表达与患者临床病理特征的关系。结果 BAG-1与NF-κB在正常结直肠黏膜、腺瘤及结直肠癌组织中的表达阳性率均呈逐渐上升趋势,3组之间阳性率比较差异具有统计学意义(P<0.05)。BAG-1与NF-κB蛋白在结直肠癌组的表达阳性率均与肿瘤分化程度、浸润深度以及淋巴结转移情况有关,差异有统计学意义(P<0.05),与患者性别、年龄无关,差异无统计学意义。结直肠癌组织中BAG-1蛋白与NF-κB蛋白表达呈正相关,差异有统计学意义。结论 BAG-1、NF-κB蛋白表达在结直肠癌发生发展中发挥着重要作用,BAG-1、NF-κB蛋白高表达均与结直肠癌恶性程度有关,可作为判断将结直肠癌生物学行为的新的生物学指标。
Objective To investigate the expression and clinical Significance of BAG-1 and NF-κB in Colorectal Carcinoma. Methods The expression of BAG-1 and NF-κB were analyzed by immunohistochemical method in 80 samples of colorectal carcinoma, 40 samples of colorectal adenoma and 40 samples of cancer adjacent colorectal mucosa. The positive expression rates of BAG-1 and NF-κB in different colorectal tissues were compared. The relationship of BAG-1 and NF-κB expression with clinicopathological features of colorectal carcinoma was analyzed. Results The expression rates of BAG-1 and NF-κBin colorectal carcinoma, colorectal adenoma, cancer adjacent colorectal mucosa increased gradually, The expression rates of the three groups was statistically significant(P<0.05). The expression rates of BAG-1 and NF-κB protein in colorectal cancer were correlated with tumor differentiation, depth of invasion and lymph node metastasis(P<0.05), and were not related to gender and age. There was a positive correlation between BAG-1 protein and NF-κB protein expression in colorectal cancer tissues. Conclusion The expression of BAG-1 and NF-κBplays an important role in the development of colorectal cancer. The high expression of BAG-1 and NF-κB protein is related to the malignant degree of colorectal cancer, and can be used as a new biological indicator to judge the biological behavior of colorectal cancer.
Objective To investigate the expression and clinical Significance of BAG-1 and NF-κB in Colorectal Carcinoma. Methods The expression of BAG-1 and NF-κB were analyzed by immunohistochemical method in 80 samples of colorectal carcinoma, 40 samples of colorectal adenoma and 40 samples of cancer adjacent colorectal mucosa. The positive expression rates of BAG-1 and NF-κB in different colorectal tissues were compared. The relationship of BAG-1 and NF-κB expression with clinicopathological features of colorectal carcinoma was analyzed. Results The expression rates of BAG-1 and NF-κBin colorectal carcinoma, colorectal adenoma, cancer adjacent colorectal mucosa increased gradually, The expression rates of the three groups was statistically significant(P<0.05). The expression rates of BAG-1 and NF-κB protein in colorectal cancer were correlated with tumor differentiation, depth of invasion and lymph node metastasis(P<0.05), and were not related to gender and age. There was a positive correlation between BAG-1 protein and NF-κB protein expression in colorectal cancer tissues. Conclusion The expression of BAG-1 and NF-κBplays an important role in the development of colorectal cancer. The high expression of BAG-1 and NF-κB protein is related to the malignant degree of colorectal cancer, and can be used as a new biological indicator to judge the biological behavior of colorectal cancer.
引文
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[10] Southern SL, Collard TJ, Urban BC, et al. BAG-1 interacts with the p50-p50 homodimeric NF-κB complex:implications for colorectal carcinogenesis[J]. Oncogene, 2012,31(22):2761-2172.
[2] Huang W, Liu Z, Zhou G, et al. Magnetic gold nanoparticle-mediated small interference RNA silencing Bag-1gene for colon carcinoma therapy[J]. Oncol Rep, 2016,35(2):978-984.
[3] Kikuchi R, Noguchi T, Takeno S, et al. Nuclear BAG-1 expression reflects malignant potential in colorectal carcinomas[J]. Br J Cancer, 2002,87(10):1136-1139.
[4] Abdullah M, Rani AA, Suddoyo AW, et al. Express of NF-κBand COX2 in colorectal cancer among native Indonesians:the role of inflammation in colorectal carcinogenesis[J]. Acta Med Indones, 2013,45(3):187-192.
[5] Takayama S, Sato T, Krajewski S, et al. Cloning and functional analysis ofBAG-1:a novel bcl-2-binding protein with anti-cell death activity[J]. Cell,1995,80(2):279-284.
[6] Ito Y, Yoshida H, Nakano K, et al. Over-expression of BAG-1 in head and neck squamous cell carcinomas(HNSCC)isassociated with cisplatin-resistance[J].J Transl Med, 2017,15(1):189.
[7] Papadakis E, Robson N, Yeomans A, et al. A combination of trastuzumab and BAG-1 inhibition synergistically targets HER2 positive breast cancer cells[J].Oncotarget, 2016,7(14):18851-18864.
[8] Hayden MS, Ghosh S. Signaling to NF-kappa B[J].Genes Dev, 2004,18:2195-2224.
[9] Xia ZB, Yuan YJ, Zhang QH, et al. Salvianolic Acid B Suppresses Inflammatory Mediator Levels by Downregulating NF-κB in a Rat Model of Rheumatoid Arthritis[J]. Med SciMonit, 2018,24:2524-2532.
[10] Southern SL, Collard TJ, Urban BC, et al. BAG-1 interacts with the p50-p50 homodimeric NF-κB complex:implications for colorectal carcinogenesis[J]. Oncogene, 2012,31(22):2761-2172.