全自动尿液分析仪复检规则的建立
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摘要
目的制定由迪瑞H-800尿干化学分析仪和IRIS IQ-200尿有形成分分析仪组成的全自动尿液分析工作站的复检规则,并验证该规则在临床中的应用效果。方法使用该工作站对1715份标本进行检测,同时对每份标本进行尿沉渣人工显微镜检测。通过对两种方法检测结果的分析,制定本实验室的尿液分析工作站复检规则,并用300份标本进行验证。结果通过对1715份标本的检测,以人工镜检结果为标准,该尿液分析工作站检测结果的假阴性率为1.22%,假阳性率为2.97%,复检率为33.41%。应用制订的复检规则对临床标本验证结果的假阴性率为1.33%,假阳性率为1.66%,复检率为35.33%。结论本实验室制定并验证了全自动尿液分析工作站复检规则,其假阴性率、假阳性率、复检率均能满足要求,该复检规则可以应用于临床工作中。
Objective To establish a re-examination rule for the automatic urine analysis workstation consisting of Derry H-800 urine dry chemical analyzer and IRIS IQ-200 urine forming analyzer, and to verify the application of this rule in the clinic. Methods A total of 1715 specimens were tested by using this workstation. At the same time, each specimen was examined by urine sediments microscopic examination. Through the analysis of the test results of two methods, re-examination rules of the laboratory′ s urine analysis workstation were developed and validated with 300 specimens. Results The results of the microscopic examination were taken as standards based on the examination of1715 specimens. The false negative rate of the test results of urine analysis workstation was 1.22%, the false positive rate was 2.97%, and the re-examination rate was 33.41%. The results of the clinical specimen validation by the application of the re-examination rules were as follows: the false negative rate was 1.33%, the false positive rate was 1.66%and the re-examination rate was 35.33%. Conclusion The laboratory has developed and validated the re-examination rules for automated urine analysis workstation. The false negative rate, false positive rate and re-examination rate all can meet the requirements. This re-examination rule can be applied in clinical practice.
Objective To establish a re-examination rule for the automatic urine analysis workstation consisting of Derry H-800 urine dry chemical analyzer and IRIS IQ-200 urine forming analyzer, and to verify the application of this rule in the clinic. Methods A total of 1715 specimens were tested by using this workstation. At the same time, each specimen was examined by urine sediments microscopic examination. Through the analysis of the test results of two methods, re-examination rules of the laboratory′ s urine analysis workstation were developed and validated with 300 specimens. Results The results of the microscopic examination were taken as standards based on the examination of1715 specimens. The false negative rate of the test results of urine analysis workstation was 1.22%, the false positive rate was 2.97%, and the re-examination rate was 33.41%. The results of the clinical specimen validation by the application of the re-examination rules were as follows: the false negative rate was 1.33%, the false positive rate was 1.66%and the re-examination rate was 35.33%. Conclusion The laboratory has developed and validated the re-examination rules for automated urine analysis workstation. The false negative rate, false positive rate and re-examination rate all can meet the requirements. This re-examination rule can be applied in clinical practice.
引文
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[9]梁红英,关植芝,杨丽红,等.建立尿液流水线尿沉渣分析仪检查结果复检规则的探讨[J].国际检验医学杂志,2016,37(13):1842-1843.
[10]梁肖云,黄镇华,张素娟,等妇女及儿童尿液分析复检规则的建立与应用[J].国际检验医学杂志,2016,37(6):797-799.
[11]张霞,李代碧,李倩.两种仪器在尿液分析中的综合应用[J].国际检验医学杂志2016,37(17):2485-2486.
[12]吕纯莉,肖代敏,朱紹玲,等.URIT-1500尿干化学分析仪与AVE-764B尿有形成分分析仪联合尿液检测复检规则的制定和应用[J].检验医学,2016,31(1):45-48.
[13]刘万超,陈龙梅,杨振华,等.爱威尿液分析流水线镜检规则制订及验证[J].检验医学,2017,32(1):22-25.
[14]陈彩虹,曹利蕉,许庆丰,等.SYSMEX全自动尿液分析系统有形成分显微镜复检规则制定与验证[J].浙江医学教育,2016,15(4):52-54.
[15]赖利华,杨春燕,傅双双.常规尿液分析复检规则再评估、修订及验证[J].重庆医学,2016,45(19):2689-2692.
[2]刘成玉,罗春丽.临床检验基础[M].第5版.北京:人民卫生出版社,2012:166-167.
[3]杨晓波,王振楠.IQ 200尿沉渣分析仪中结果审核的必要性[J].中国医药导报,2011,8(6):153-154.
[4]丛玉隆.尿液有形成分检查及镜检筛选标准的制定[J].中华检验医学杂志,2011,34(6):481-482.
[5]王银锋,李锋,李想,等.尿常规复检规则的建立与验证[J].宁夏医学杂志,2014,36(5):429-431.
[6]鲍蕴文,孙龙乔子.IQ-200全自动尿沉渣分析仪镜检复检规则制订与应用[J].检验医学与临床,2013,10(21):2865-2867.
[7]吴鹏,谢世英,黄茜.迪瑞尿沉渣流水线用于尿液有形成分镜检筛选标准的研究[J].检验医学与临床,2015,12(24):3660-3663.
[8]马俊龙,丛玉隆,陆玉静,等.尿干化学与流式细胞术联合用于尿液有形成分镜检筛选的研究与应用[J].中华检验医学杂志,2011,34(6):494-500.
[9]梁红英,关植芝,杨丽红,等.建立尿液流水线尿沉渣分析仪检查结果复检规则的探讨[J].国际检验医学杂志,2016,37(13):1842-1843.
[10]梁肖云,黄镇华,张素娟,等妇女及儿童尿液分析复检规则的建立与应用[J].国际检验医学杂志,2016,37(6):797-799.
[11]张霞,李代碧,李倩.两种仪器在尿液分析中的综合应用[J].国际检验医学杂志2016,37(17):2485-2486.
[12]吕纯莉,肖代敏,朱紹玲,等.URIT-1500尿干化学分析仪与AVE-764B尿有形成分分析仪联合尿液检测复检规则的制定和应用[J].检验医学,2016,31(1):45-48.
[13]刘万超,陈龙梅,杨振华,等.爱威尿液分析流水线镜检规则制订及验证[J].检验医学,2017,32(1):22-25.
[14]陈彩虹,曹利蕉,许庆丰,等.SYSMEX全自动尿液分析系统有形成分显微镜复检规则制定与验证[J].浙江医学教育,2016,15(4):52-54.
[15]赖利华,杨春燕,傅双双.常规尿液分析复检规则再评估、修订及验证[J].重庆医学,2016,45(19):2689-2692.