UF-1000i尿液沉渣分析仪在尿液管型检测中的应用分析
|
摘要
目的分析UF-1000i尿沉渣分析仪检测尿液管型存在的假阳性、假阴性因素,提出相应的解决方案。方法随机选取本院2018年1～4月门诊、住院尿液标本2130例分别用干化学分析法、UF-1000i分析仪法和人工显微镜法进行尿液有形成分检测,并收集有关管型的数据。以人工显微镜法判定管型检测真阳性、真阴性标准。结果 UF-1000i尿液分析仪法和显微镜镜检法的阳性率分别是11.60%、4.51%,两者比较差异有统计学意义(P <0.05)。UF-1000i分析仪法检测管型的假阳性率为7.09%,假阴性率为0.28%。引起UF-1000i假阳性的因素有黏液丝,各种结晶、细菌成团,白细胞、上皮细胞成团,分别占比例为60.93%、23.84%、15.23%。引起UF-1000i假阴性的因素为管型短小或溶解,或者标本的电导率低出现漏诊及管型内颗粒无法被荧光染料染色造成仪器漏检。结论UF-1000i尿沉渣分析仪检测尿液管型需结合显微镜镜检法,并进一步优化仪器性能以提高结果的准确性。
Objective To analyze the false-positive and false-negative factors of urine castdetection by UF-1000 i urine sediment analyzer, and propose corresponding solutions. Methods A total of 2130 urine samples from outpatient and inpatient patients in our hospital from January to April 2018 were randomly selected. Dry chemical analysis, UF-1000 i analyzer and artificial microscopy were used to detect the urine formed elements, and the data of urine cast were collected. Artificial microscopy was used to determine the true-positive and truenegative criteria for urine cast test. Results The positive rates of UF-1000 i urine analyzer and microscopic examination were 11.60% and 4.51% respectively, the difference was statistically significant(P < 0.05). The false positive rate and false negative rate of UF-1000 i analyzer were 7.09% and 0.28% respectively. Mucous filaments, various crystals, bacterial clusters, white blood cells and epithelial cells were the main factors caused false positive of UF-1000 i, accounted for 60.93%, 23.84% and 15.23% respectively. The causes of UF-1000 i false negative were the cast was short or dissolved, or the conductivity of the specimen was low, and the missing specimens and the particles in the cast cannot be stained by fluorescent dyes. Conclusion The UF-1000 i urine sediment analyzer should be combined with microscopy to detect the urine cast, and further optimize the performance of the instrument to improve the accuracy of the results.
Objective To analyze the false-positive and false-negative factors of urine castdetection by UF-1000 i urine sediment analyzer, and propose corresponding solutions. Methods A total of 2130 urine samples from outpatient and inpatient patients in our hospital from January to April 2018 were randomly selected. Dry chemical analysis, UF-1000 i analyzer and artificial microscopy were used to detect the urine formed elements, and the data of urine cast were collected. Artificial microscopy was used to determine the true-positive and truenegative criteria for urine cast test. Results The positive rates of UF-1000 i urine analyzer and microscopic examination were 11.60% and 4.51% respectively, the difference was statistically significant(P < 0.05). The false positive rate and false negative rate of UF-1000 i analyzer were 7.09% and 0.28% respectively. Mucous filaments, various crystals, bacterial clusters, white blood cells and epithelial cells were the main factors caused false positive of UF-1000 i, accounted for 60.93%, 23.84% and 15.23% respectively. The causes of UF-1000 i false negative were the cast was short or dissolved, or the conductivity of the specimen was low, and the missing specimens and the particles in the cast cannot be stained by fluorescent dyes. Conclusion The UF-1000 i urine sediment analyzer should be combined with microscopy to detect the urine cast, and further optimize the performance of the instrument to improve the accuracy of the results.
引文
[1]纪凤卿,滕菁,林丽云,等.UF-1000i检测尿液红细胞的应用分析[J].中国医药科学,2017,7(21):124-126,145.
[2]尚红,王毓三,申子瑜.全国临床检验操作规程[M].第4版.北京:人民卫生出版社,2015:171.
[3]秦建军.UF-1000i尿有形成分分析仪检测尿管型的评价[J].哈尔滨医药,2013,33(3):213.
[4]王桂英.LX-3000型全自动尿液分析仪检测阴性标本与尿沉渣镜检结果对比分析[J].青海医药杂志,2015,58(5):45-46.
[5]方雯丹,甘志忠,黄晓荣,等.全自动尿沉渣分析仪Sysmex UF-1000i的性能评价[J].检验医学与临床,2015,12(6):770-772.
[6]叶晓慧.Sysmex UF-1000全自动尿沉渣分析仪与显微镜检测尿液中管型的对比分析[J].中国卫生标准管理,2017,8(4):114-115.
[7]鲍蕴文,孙龙乔子.IQ-200.全自动尿沉渣分析仪镜检复检规则制订与应用[J].检验医学与临床,2013,10(21):2865-2867.
[8]邱峰,陆秀芬,刘燕.联合应用尿液干化学法与尿沉渣镜检法进行白细胞检验的价值分析[J].中国医药科学,2018,8(5):149-151.
[9]蒋平平,李冬,杨凡.pH值和尿蛋白水平对尿液分析仪管型检出率的影响[J].中国卫生标准管理,2017,8(17):96-97.
[10]叶晓慧.Sysmex UF-1000全自动尿沉渣分析仪与显微镜检测尿液中管型的对比分析[J].中国卫生标准管理,2017,8(4):114-115.
[11]张晓峰.IQ200尿沉渣分析仪检测肾小管管型与手工镜检符合率对检测结果的影响[J].中国继续医学教育,2016,8(25):44-45.
[12]Manoni F1,Gessoni G,Alessio MG,et al.Gender’s equality in evaluation of urine particles:Results of a multicenter study of the Italian Urinalysis Group[J].Clin Chim Acta,2014,427:1-5.
[13]马海琴.试带法尿蛋白阴性而镜下管型异常的探讨[J].现代预防医学,2015,42(12):2236-2237.
[14]代瑛,张喆,刘昕阳,等.联合应用尿液干化学法及尿沉渣仪检测尿液管型分析[J].国际检验医学杂志,2014,35(5):611-612.
[15]Zhao F,Jin Y,Chen X,et al.Clinical application of UF-1000i in combination with AX-4280 for the screening test ability of urinary formed elements[J].J Clin Pathol,2013,66(3):229-231.
[16]Jiang T,Chen P,Ouyang J,et al.Urine particles analysis:Performance evaluation of Sysmex UF-1000i and comparison among urine flow cytometer,dipstick,and visual microscopic examination[J].Scand J Clin Lab Invest,2011,71(1):30-37.
[2]尚红,王毓三,申子瑜.全国临床检验操作规程[M].第4版.北京:人民卫生出版社,2015:171.
[3]秦建军.UF-1000i尿有形成分分析仪检测尿管型的评价[J].哈尔滨医药,2013,33(3):213.
[4]王桂英.LX-3000型全自动尿液分析仪检测阴性标本与尿沉渣镜检结果对比分析[J].青海医药杂志,2015,58(5):45-46.
[5]方雯丹,甘志忠,黄晓荣,等.全自动尿沉渣分析仪Sysmex UF-1000i的性能评价[J].检验医学与临床,2015,12(6):770-772.
[6]叶晓慧.Sysmex UF-1000全自动尿沉渣分析仪与显微镜检测尿液中管型的对比分析[J].中国卫生标准管理,2017,8(4):114-115.
[7]鲍蕴文,孙龙乔子.IQ-200.全自动尿沉渣分析仪镜检复检规则制订与应用[J].检验医学与临床,2013,10(21):2865-2867.
[8]邱峰,陆秀芬,刘燕.联合应用尿液干化学法与尿沉渣镜检法进行白细胞检验的价值分析[J].中国医药科学,2018,8(5):149-151.
[9]蒋平平,李冬,杨凡.pH值和尿蛋白水平对尿液分析仪管型检出率的影响[J].中国卫生标准管理,2017,8(17):96-97.
[10]叶晓慧.Sysmex UF-1000全自动尿沉渣分析仪与显微镜检测尿液中管型的对比分析[J].中国卫生标准管理,2017,8(4):114-115.
[11]张晓峰.IQ200尿沉渣分析仪检测肾小管管型与手工镜检符合率对检测结果的影响[J].中国继续医学教育,2016,8(25):44-45.
[12]Manoni F1,Gessoni G,Alessio MG,et al.Gender’s equality in evaluation of urine particles:Results of a multicenter study of the Italian Urinalysis Group[J].Clin Chim Acta,2014,427:1-5.
[13]马海琴.试带法尿蛋白阴性而镜下管型异常的探讨[J].现代预防医学,2015,42(12):2236-2237.
[14]代瑛,张喆,刘昕阳,等.联合应用尿液干化学法及尿沉渣仪检测尿液管型分析[J].国际检验医学杂志,2014,35(5):611-612.
[15]Zhao F,Jin Y,Chen X,et al.Clinical application of UF-1000i in combination with AX-4280 for the screening test ability of urinary formed elements[J].J Clin Pathol,2013,66(3):229-231.
[16]Jiang T,Chen P,Ouyang J,et al.Urine particles analysis:Performance evaluation of Sysmex UF-1000i and comparison among urine flow cytometer,dipstick,and visual microscopic examination[J].Scand J Clin Lab Invest,2011,71(1):30-37.