不同产地及生长年限曼地亚红豆杉中10-脱乙酰基巴卡亭Ⅲ、三尖杉宁碱、紫杉醇量的比较分析
|
摘要
目的分析和比较5个不同产地、不同生长年限曼地亚红豆杉中3种紫杉烷类活性成分量的差异。方法利用甲醇和三氯甲烷混合液超声浸提后再用甲醇溶解得到样品溶液;通过高效液相色谱法测定和比较10-脱乙酰基巴卡亭Ⅲ(10-DAB Ⅲ)、三尖杉宁碱和紫杉醇量及3者总量。结果不同产地、不同生长年限的曼地亚红豆杉中3种紫杉烷类及其总量间存在极显著性差异(P<0.01);10-DAB Ⅲ、三尖杉宁碱、紫杉醇和3种紫杉烷类总量相对较高的3个产地及生长年限分别依次为HK4>SM3>SM2、ZL3>HK4>ZL4、ZL3>SM2>HK4、HK4>ZL3>ZL6。除10-DAB Ⅲ与三尖杉宁碱量间不存在显著的相关性外,其余各成分间均存在极显著的正相关关系(P<0.01);不同产地及生长年限间紫杉醇与3种紫杉烷类总量间呈现出同升同降的趋势;聚类分析表明福建福州产区紫杉醇量具有一定的特殊性,而河南开封3种紫杉烷类总量有别于其他产区。结论产地和生长年限对曼地亚红豆杉中3种紫杉烷类量的变化具有明显的影响,可以通过对生物合成途径进行调控,继而达到获得更多紫杉醇原料药的目的。
Objective To analysis and compare the content differences of three effective components of taxanes of Taxus madia Rehd.from five different habitats and growth years by High Performance Liquid Chromatography(HPLC).Methods The mixed liquor of CH3 OH and CHCl3 was used for the ultrasonic extraction of 10-deacetyl-bacratin Ⅲ(10-DAB Ⅲ),cephalomannine and taxol before being dissolved by CH3 OH to obtain sample solution.By HPLC,the contents of three effective components were performed and compared.Results There were extremely significantly differences(P < 0.01) among the content of three effective components of taxanes of Taxus madia Rehd.from different habitats and growth years.Three different habitats and growth years of 10-DAB Ⅲ,cephalomannine,taxol,and taxanes that have relative higher total content were HK4 > SM3 > SM2,ZL3 > HK4 > ZL4,ZL3 > SM2 > HK4,HK4 > ZL3 > ZL6,respectively.Except for the contents of 10-DAB Ⅲ and cephalomannine,there were significant positive correlations among the contents of other components(P < 0.01).The contents of taxol would rise or decline when the total contents of three kinds of taxanes rised or declined among different habitats and growth years.The clustering analysis results showed that the contents of taxol have certain peculiarity.In Fuzhou of Fujian,while the total contents of three kinds of taxanes were different from others in Kaifen of Henan.Conclusion Habitants and growth years have a significant impact on the contents change of three effective components of taxanes from Taxus madia Rehd..Therefore,regulation and control of biosynthetic pathway by choosing habitat conditions and harvest years should be considered,in order to obtain more crude drug of taxol in T.madia.
Objective To analysis and compare the content differences of three effective components of taxanes of Taxus madia Rehd.from five different habitats and growth years by High Performance Liquid Chromatography(HPLC).Methods The mixed liquor of CH3 OH and CHCl3 was used for the ultrasonic extraction of 10-deacetyl-bacratin Ⅲ(10-DAB Ⅲ),cephalomannine and taxol before being dissolved by CH3 OH to obtain sample solution.By HPLC,the contents of three effective components were performed and compared.Results There were extremely significantly differences(P < 0.01) among the content of three effective components of taxanes of Taxus madia Rehd.from different habitats and growth years.Three different habitats and growth years of 10-DAB Ⅲ,cephalomannine,taxol,and taxanes that have relative higher total content were HK4 > SM3 > SM2,ZL3 > HK4 > ZL4,ZL3 > SM2 > HK4,HK4 > ZL3 > ZL6,respectively.Except for the contents of 10-DAB Ⅲ and cephalomannine,there were significant positive correlations among the contents of other components(P < 0.01).The contents of taxol would rise or decline when the total contents of three kinds of taxanes rised or declined among different habitats and growth years.The clustering analysis results showed that the contents of taxol have certain peculiarity.In Fuzhou of Fujian,while the total contents of three kinds of taxanes were different from others in Kaifen of Henan.Conclusion Habitants and growth years have a significant impact on the contents change of three effective components of taxanes from Taxus madia Rehd..Therefore,regulation and control of biosynthetic pathway by choosing habitat conditions and harvest years should be considered,in order to obtain more crude drug of taxol in T.madia.
引文
[1]程立超,谢孝坤,韩小冰,等.红豆杉属植物中紫杉烷类物质含量影响因素综述[J].中国林副特产,2013,126(5):103-106.
[2]彭涛,林军,胡海峰,等.生物合成紫杉醇的研究进展[J].世界临床药物,2015,36(3):197-203.
[3]常醉,周志强,夏春梅,等.天然东北红豆杉枝中紫杉醇和三尖杉宁碱含量变化特征[J].北京林业大学学报,2012,34(2):71-77.
[4]Onrubia M,Cusido R M,Ramirez K,et al.Bioprocessing of plant in vitro systems for the mass production of pharmaceutically important metabolites:paclitaxel and its derivative[J].Curr Med Chem,2013,20:880-891.
[5]冯巍,谈锋,谢峻.曼地亚红豆杉研究进展[J].中草药,2007,38(10):1589-1593.
[6]董艳,孙利鑫,尹翠,等.曼地亚红豆杉栽培管理及应用的研究进展[J].安徽农业科学,2015,43(29):14-16.
[7]黄玺,李远.不同采集时间曼地亚红豆杉中紫杉醇含量分析[J].现代医药卫生,2013,29(12):1806-1808.
[8]胡蕾,梅忠,李真.不同诱导子对曼地亚红豆杉紫杉醇含量的影响[J].现代农业科技,2012(5):12-13.
[9]刘柿良,杨秀利,马明东,等.曼地亚红豆杉(Taxusmedia cv“Hicksii”)栽培基质中适宜的锰、锌、铜浓度及采收时期研究[J].植物营养与肥料学报,2015,21(2):439-448.
[10]陈立国,刘程惠,何克江,等.HPLC法对5种红豆杉属植物中紫杉烷类化合物成分的分析[J].分析实验室,2007,26:58-61.
[11]王春丽,梁宗锁.外源刺激对植物次生代谢的调节及其信号转导途径研究进展[J].西北植物学报,2009,29(5):867-873.
[12]段传人,王伯初,徐世荣.环境应力对植物次生代谢产物形成的作用[J].重庆大学学报,2003,26(10):67-71.
[13]宋秋烨.南方红豆杉与引种德国曼地亚红豆杉茎、叶中紫杉醇和10-脱乙酰巴卡亭III含量的比较[J].西北药学杂志,2014,29(2):129-132.
[14]高银祥,杨逢建,张玉红,等.南方红豆杉枝叶中6种紫杉烷类化合物含量季节变化[J].植物研究,2014,34(2):266-270.
[15]樊如强,傅宏征,金学英,等.刺五加有效成分提取工艺考察及不同产地刺五加中有效成分量的比较[J].中草药,2014,45(2):3051-3054.
[16]唐慧勤,龙颖,董威,等.产地、生长期、商品规格等不同因素对罗汉果止咳作用的影响[J].中草药,2015,46(20):260-264.
[17]朱慧芳,郑文龙,王玉亮,等.三种红豆杉属植物中紫杉烷类化合物含量的检测与分析[J].上海交通大学学报:农业科学版,2010,28(1):9-13.
[18]王达明,周云,张裕农,等.不同海拔高度及植被类型的云南红豆杉林木枝叶紫杉烷含量分异性研究[J].西部林业科学,2008,37(4):1-7.
[19]柏培磊,孙启武,蒋继宏,等.不同产地南方红豆杉枝叶的紫杉醇含量及其指纹图谱的比较分析[J].植物资源与环境学报,2012,21(1):64-69.
[20]高明波,阮成江,李贺,等.紫杉醇和紫杉烷的生物合成途径研究进展[J].中国药学杂志,2010,45(24):1900-1903.
[2]彭涛,林军,胡海峰,等.生物合成紫杉醇的研究进展[J].世界临床药物,2015,36(3):197-203.
[3]常醉,周志强,夏春梅,等.天然东北红豆杉枝中紫杉醇和三尖杉宁碱含量变化特征[J].北京林业大学学报,2012,34(2):71-77.
[4]Onrubia M,Cusido R M,Ramirez K,et al.Bioprocessing of plant in vitro systems for the mass production of pharmaceutically important metabolites:paclitaxel and its derivative[J].Curr Med Chem,2013,20:880-891.
[5]冯巍,谈锋,谢峻.曼地亚红豆杉研究进展[J].中草药,2007,38(10):1589-1593.
[6]董艳,孙利鑫,尹翠,等.曼地亚红豆杉栽培管理及应用的研究进展[J].安徽农业科学,2015,43(29):14-16.
[7]黄玺,李远.不同采集时间曼地亚红豆杉中紫杉醇含量分析[J].现代医药卫生,2013,29(12):1806-1808.
[8]胡蕾,梅忠,李真.不同诱导子对曼地亚红豆杉紫杉醇含量的影响[J].现代农业科技,2012(5):12-13.
[9]刘柿良,杨秀利,马明东,等.曼地亚红豆杉(Taxusmedia cv“Hicksii”)栽培基质中适宜的锰、锌、铜浓度及采收时期研究[J].植物营养与肥料学报,2015,21(2):439-448.
[10]陈立国,刘程惠,何克江,等.HPLC法对5种红豆杉属植物中紫杉烷类化合物成分的分析[J].分析实验室,2007,26:58-61.
[11]王春丽,梁宗锁.外源刺激对植物次生代谢的调节及其信号转导途径研究进展[J].西北植物学报,2009,29(5):867-873.
[12]段传人,王伯初,徐世荣.环境应力对植物次生代谢产物形成的作用[J].重庆大学学报,2003,26(10):67-71.
[13]宋秋烨.南方红豆杉与引种德国曼地亚红豆杉茎、叶中紫杉醇和10-脱乙酰巴卡亭III含量的比较[J].西北药学杂志,2014,29(2):129-132.
[14]高银祥,杨逢建,张玉红,等.南方红豆杉枝叶中6种紫杉烷类化合物含量季节变化[J].植物研究,2014,34(2):266-270.
[15]樊如强,傅宏征,金学英,等.刺五加有效成分提取工艺考察及不同产地刺五加中有效成分量的比较[J].中草药,2014,45(2):3051-3054.
[16]唐慧勤,龙颖,董威,等.产地、生长期、商品规格等不同因素对罗汉果止咳作用的影响[J].中草药,2015,46(20):260-264.
[17]朱慧芳,郑文龙,王玉亮,等.三种红豆杉属植物中紫杉烷类化合物含量的检测与分析[J].上海交通大学学报:农业科学版,2010,28(1):9-13.
[18]王达明,周云,张裕农,等.不同海拔高度及植被类型的云南红豆杉林木枝叶紫杉烷含量分异性研究[J].西部林业科学,2008,37(4):1-7.
[19]柏培磊,孙启武,蒋继宏,等.不同产地南方红豆杉枝叶的紫杉醇含量及其指纹图谱的比较分析[J].植物资源与环境学报,2012,21(1):64-69.
[20]高明波,阮成江,李贺,等.紫杉醇和紫杉烷的生物合成途径研究进展[J].中国药学杂志,2010,45(24):1900-1903.