野艾蒿精油化学分离各组分对小菜蛾的杀虫活性
|
摘要
通过化学分离的方法从野艾蒿精油中获得碱性、强酸性、弱酸性、中性和醛酮类5个组分,为了明确不同组分的杀虫活性,测定了5个组分对小菜蛾3龄幼虫的触杀、熏蒸、胃毒活性及对小菜蛾成虫的驱避活性。试验结果表明:中性组分对小菜蛾表现出明显的触杀、熏蒸、胃毒和驱避作用,在处理后10 h,触杀和熏蒸处理的校正死亡率分别达到100%和97. 62%;强酸性和弱酸性组分的杀虫效果不明显;碱性组分对小菜蛾未表现出触杀、熏蒸和胃毒活性;醛酮类组分对小菜蛾未表现出触杀和熏蒸作用。总的来看,野艾蒿精油中性组分对小菜蛾的杀虫活性最好。
In this study,five components of alkalinity,strong acidic,weak acidic and neutral components,and aldehydes and ketones were obtained from essential oil of Artemisia lavandulaefolia by chemical separation. To determine the insecticidal activity of different components of A. lavandulaefolia essential oil,contact,fumigation,stomach poisoning and repellent activity of the five components against Plutella xylostella were tested. The results showed that the neutral component showed obvious contact,fumigation,stomach poisoning and repellent effects on P. xylostella,and the corrected mortality rates of contact and fumigation reached 100% and 97. 62%,respectively,after 10 h treatment. The strong acidic and weak acidic components had no obvious insecticidal effect. Alkalinity did not show contact,fumigation and stomach poisoning activity against P. xylostella. Aldehydes and ketones had no contact and fumigation activity. In general,the neutral component of A. lavandulaefolia essential oil had the best insecticidal activity against P. xylostella.
In this study,five components of alkalinity,strong acidic,weak acidic and neutral components,and aldehydes and ketones were obtained from essential oil of Artemisia lavandulaefolia by chemical separation. To determine the insecticidal activity of different components of A. lavandulaefolia essential oil,contact,fumigation,stomach poisoning and repellent activity of the five components against Plutella xylostella were tested. The results showed that the neutral component showed obvious contact,fumigation,stomach poisoning and repellent effects on P. xylostella,and the corrected mortality rates of contact and fumigation reached 100% and 97. 62%,respectively,after 10 h treatment. The strong acidic and weak acidic components had no obvious insecticidal effect. Alkalinity did not show contact,fumigation and stomach poisoning activity against P. xylostella. Aldehydes and ketones had no contact and fumigation activity. In general,the neutral component of A. lavandulaefolia essential oil had the best insecticidal activity against P. xylostella.
引文
[1]林有润.中国植物志[M].北京:科学出版社,1991:87-117.
[2]吉林省中医中药研究所.长白山植物药志[M].长春:吉林人民出版社,1982:1122.
[3]新疆植物志编辑委员会.新疆植物志(第五卷)[M].乌鲁木齐:新疆科技卫生出版社,1999:146.
[4]朱亚民.内蒙古植物药志(第三卷)[M].呼和浩特:内蒙古人民出版社,1989:175.
[5]张璐敏,吕学维,邵邻相,等.野艾蒿挥发油对He La癌细胞形态与结构的影响[J].广西植物,2014,34(3):393-397.
[6] Jeong D C,Youn H K,Ji Y K. Essential oil and 1,8-Cineole from Artemisia lavandulaefolia induces apoptosis in KB Cells via mitoehondrial stressand caspase activation[J]. Food Sci Biotechnol,2010,19(1):185-191.
[7]王登奎,吴刚,程向晖,等.野艾蒿中氨基酸、维生素、微量元素的含量分析[J].中成药,2006,28(11):1658-1660.
[8]沈霞,张绝红,袁慧慧,等.响应面分析法优化艾叶粗多糖提取工艺的研究[J].中成药,2010,32(1):48-51.
[9]谢强敏,卞如镰,杨秋火,等.艾叶油的呼吸系统药理研究I-支气管扩张、镇咳和祛痰作用[J].中国现代应用药学杂志,1999,16(4):16-19.
[10]江贵波,曾任森,陈少雄,等.中药野艾挥发性物质的抗菌活性与成分鉴定[J].沈阳农业大学学报,2008,39(4):495-498.
[11]韩小冰,谢孝坤,乔润喜.五种蒿属植物对舞毒蛾的毒力测定[J].中国林副特产,2011(6):30-35.
[12]莫茂军,吴洪基,韩诗畴,等.16种植物乙醇提取物对桔全爪螨的生物活性测定[J].环境昆虫学报,2008,30(1):42-47.
[13]袁海滨.野艾蒿(Artemisia lavandulaefolia D C.)精油的生物活性、作用机制及安全性研究[D].长春:东北师范大学,2010.
[14]袁海滨,李玉双,赵欣阳,等.3种植物精油对绿豆象成虫的熏蒸及驱避活性[J].吉林农业大学学报,2017,39(1):28-31.
[15]袁海滨,刘莹,丁玉骁,等.野艾蒿精油对双斑萤叶甲成虫的熏蒸活性及体内酶活力的影响[J].吉林农业大学学报,2014,36(1):30-35.
[16]赵欣阳,黄星,葛思妍,等.小菜蛾触角感器的扫描电镜观察[J].吉林农业大学学报,2018,40(1):58-62.
[17] Attique M,Khaliq A,Sayyed A. Could resistance to insecticides in Plutella xylostella(Lep.,Plutellidae)be overcome by insecticide mixtures[J]. Journal of Applied Entomology,2006,130(2):122-127.
[18] Furlong M J,Wright D J,Dosdall L M. Diamondback moth ecology and management:problems,progress,and prospects[J]. Annual Review of Entomology,2013,58:517-541.
[19] Talekar N,Shelton A. Biology,ecology,and management of the diamondback moth[J]. Annual Review of Entomology,1993,38:275-301.
[20]袁海滨,王晶,赵欣阳,等.黄花蒿精油对绿豆象成虫体内酶活力的影响[J].吉林农业大学学报,2016,38(4):416-419.
[21]袁海滨,杨洁,陈骧,等.黄花蒿精油对双斑萤叶甲的熏蒸活性及体内几种酶活力的影响[J].吉林农业大学学报,2013,35(1):30-35.
[22]孙中武.植物化学[M].哈尔滨:东北林业大学出版社,2000:209-220.
[23] Jilani G,Saxen R C. Repellent and feeding detefrent effects of turmeric oil,neem oil,and a neem-based insecticide against lesser grain borer(Coleoptera:Bostrychidae)[J]. Journal of Economic Entomology,1990,83(2):629-634.
[24]高红明,王兆龙,张彪,等.植物提取液对菜青虫的杀虫活性研究[J].江苏农业研究,1999,20(4):32-34.
[25]郭达伟.假臭草精油对小菜蛾的生物活性及其作用机理研究[D].北京:中国农业科学院,2008.
[26]孙红霞,占志雄,吴珍泉,等.种植物精油对小菜蛾幼虫的触杀作用及两种酶活性的影响[J].福建农业学报,2008,23(3):274-276.
[27]赵晓燕,侯有明.龙柏精油对小菜蛾的生物活性[J].昆虫知识,2006,43(1):57-60.
[28]纪明山,范艳菊,祁之秋,等.30种中药材提取物对小菜蛾生物活性测定[J].江苏农业科学,2010(2):115-116.
[29]庄世宏.花椒精油提取及其生物活性测定研究[D].杨凌:西北农林科技大学,2002.
[30]唐为芳,苏丽,李兰青,等.柠檬桉精油对小菜蛾幼虫生物活性的研究[J].中国园艺文摘,2010(4):175-176.
[31]覃伟权,张茂新,凌冰,等.3种热带杂草挥发油干扰小菜蛾行为的研究[J].华南农业大学学报,2004,25(4):39-42.
[32] Liu Z L,Chu S S,Liu Q R. Chemical composition and insecticidal activity against Sitophilus zeamais of the essential oils of Artemisia capillaris and Artemisia mongolica[J]. Molecules,2010,15(4):2600-2608.
[33]邓治邦,刘群,杨智蕴,等.野艾蒿挥发油化学成分的研究[J].东北师大学报(自然科学版),1987(3):73-74.
[34] Cha J D,Jeong M R,Choi H J,et al. Chemical composition and antimicrobial activity of the essential oil of Artemisia lavandulaefolia[J]. Planta Med,2005,71:575-577.
[35]王晓琴,周成江,张娜,等.野艾蒿化学成分研究[J].中药材,2011,34(2):234.
[36]马麟,韦炳华,胡黎.野艾蒿的化学成分研究[J].广州中医药大学学报,2012,29(4):450-453.
[2]吉林省中医中药研究所.长白山植物药志[M].长春:吉林人民出版社,1982:1122.
[3]新疆植物志编辑委员会.新疆植物志(第五卷)[M].乌鲁木齐:新疆科技卫生出版社,1999:146.
[4]朱亚民.内蒙古植物药志(第三卷)[M].呼和浩特:内蒙古人民出版社,1989:175.
[5]张璐敏,吕学维,邵邻相,等.野艾蒿挥发油对He La癌细胞形态与结构的影响[J].广西植物,2014,34(3):393-397.
[6] Jeong D C,Youn H K,Ji Y K. Essential oil and 1,8-Cineole from Artemisia lavandulaefolia induces apoptosis in KB Cells via mitoehondrial stressand caspase activation[J]. Food Sci Biotechnol,2010,19(1):185-191.
[7]王登奎,吴刚,程向晖,等.野艾蒿中氨基酸、维生素、微量元素的含量分析[J].中成药,2006,28(11):1658-1660.
[8]沈霞,张绝红,袁慧慧,等.响应面分析法优化艾叶粗多糖提取工艺的研究[J].中成药,2010,32(1):48-51.
[9]谢强敏,卞如镰,杨秋火,等.艾叶油的呼吸系统药理研究I-支气管扩张、镇咳和祛痰作用[J].中国现代应用药学杂志,1999,16(4):16-19.
[10]江贵波,曾任森,陈少雄,等.中药野艾挥发性物质的抗菌活性与成分鉴定[J].沈阳农业大学学报,2008,39(4):495-498.
[11]韩小冰,谢孝坤,乔润喜.五种蒿属植物对舞毒蛾的毒力测定[J].中国林副特产,2011(6):30-35.
[12]莫茂军,吴洪基,韩诗畴,等.16种植物乙醇提取物对桔全爪螨的生物活性测定[J].环境昆虫学报,2008,30(1):42-47.
[13]袁海滨.野艾蒿(Artemisia lavandulaefolia D C.)精油的生物活性、作用机制及安全性研究[D].长春:东北师范大学,2010.
[14]袁海滨,李玉双,赵欣阳,等.3种植物精油对绿豆象成虫的熏蒸及驱避活性[J].吉林农业大学学报,2017,39(1):28-31.
[15]袁海滨,刘莹,丁玉骁,等.野艾蒿精油对双斑萤叶甲成虫的熏蒸活性及体内酶活力的影响[J].吉林农业大学学报,2014,36(1):30-35.
[16]赵欣阳,黄星,葛思妍,等.小菜蛾触角感器的扫描电镜观察[J].吉林农业大学学报,2018,40(1):58-62.
[17] Attique M,Khaliq A,Sayyed A. Could resistance to insecticides in Plutella xylostella(Lep.,Plutellidae)be overcome by insecticide mixtures[J]. Journal of Applied Entomology,2006,130(2):122-127.
[18] Furlong M J,Wright D J,Dosdall L M. Diamondback moth ecology and management:problems,progress,and prospects[J]. Annual Review of Entomology,2013,58:517-541.
[19] Talekar N,Shelton A. Biology,ecology,and management of the diamondback moth[J]. Annual Review of Entomology,1993,38:275-301.
[20]袁海滨,王晶,赵欣阳,等.黄花蒿精油对绿豆象成虫体内酶活力的影响[J].吉林农业大学学报,2016,38(4):416-419.
[21]袁海滨,杨洁,陈骧,等.黄花蒿精油对双斑萤叶甲的熏蒸活性及体内几种酶活力的影响[J].吉林农业大学学报,2013,35(1):30-35.
[22]孙中武.植物化学[M].哈尔滨:东北林业大学出版社,2000:209-220.
[23] Jilani G,Saxen R C. Repellent and feeding detefrent effects of turmeric oil,neem oil,and a neem-based insecticide against lesser grain borer(Coleoptera:Bostrychidae)[J]. Journal of Economic Entomology,1990,83(2):629-634.
[24]高红明,王兆龙,张彪,等.植物提取液对菜青虫的杀虫活性研究[J].江苏农业研究,1999,20(4):32-34.
[25]郭达伟.假臭草精油对小菜蛾的生物活性及其作用机理研究[D].北京:中国农业科学院,2008.
[26]孙红霞,占志雄,吴珍泉,等.种植物精油对小菜蛾幼虫的触杀作用及两种酶活性的影响[J].福建农业学报,2008,23(3):274-276.
[27]赵晓燕,侯有明.龙柏精油对小菜蛾的生物活性[J].昆虫知识,2006,43(1):57-60.
[28]纪明山,范艳菊,祁之秋,等.30种中药材提取物对小菜蛾生物活性测定[J].江苏农业科学,2010(2):115-116.
[29]庄世宏.花椒精油提取及其生物活性测定研究[D].杨凌:西北农林科技大学,2002.
[30]唐为芳,苏丽,李兰青,等.柠檬桉精油对小菜蛾幼虫生物活性的研究[J].中国园艺文摘,2010(4):175-176.
[31]覃伟权,张茂新,凌冰,等.3种热带杂草挥发油干扰小菜蛾行为的研究[J].华南农业大学学报,2004,25(4):39-42.
[32] Liu Z L,Chu S S,Liu Q R. Chemical composition and insecticidal activity against Sitophilus zeamais of the essential oils of Artemisia capillaris and Artemisia mongolica[J]. Molecules,2010,15(4):2600-2608.
[33]邓治邦,刘群,杨智蕴,等.野艾蒿挥发油化学成分的研究[J].东北师大学报(自然科学版),1987(3):73-74.
[34] Cha J D,Jeong M R,Choi H J,et al. Chemical composition and antimicrobial activity of the essential oil of Artemisia lavandulaefolia[J]. Planta Med,2005,71:575-577.
[35]王晓琴,周成江,张娜,等.野艾蒿化学成分研究[J].中药材,2011,34(2):234.
[36]马麟,韦炳华,胡黎.野艾蒿的化学成分研究[J].广州中医药大学学报,2012,29(4):450-453.