油污土壤植物与真菌联合生物修复的研究
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摘要
利用具有石油烃降解能力的真菌与植物复配,探讨不同植物与真菌联合修复油污土壤的效果。将4种植物(玉米、玉米草、黑麦草、紫花苜蓿)与2种真菌(Fungal sp.、Stachybotrys sp.)在5种浓度油污土壤(0.1%,0.5%,1%,1.5%,2.0%)中进行单接种及双接种种植试验,以7d为单位测定石油烃降解率,筛选出对各浓度油污土壤修复效果最佳的复配方案。结果表明,玉米、玉米草和黑麦草单接种处理原油降解效果较好,且在油污浓度为1%时,3种植物均达到最高的降解效率。当油污浓度高于1.5%时,紫花苜蓿混合接种处理对油污的降解率明显升高,最大可达到87%。紫花苜蓿在混合接种条件下更适合高浓度油污土壤的生物修复,推广价值更大。
The petroleum degradation of compound with four plants and two petroleum degraded fungus was discussed.Single or double of two fungus(Fungal sp.,Stachybotrys sp.) were respectively inoculated with four plants(corn,teosinte,ryegrass,alfalfa) into five oil concentrations(0.1%,0.5%,1%,1.5%,2.0%) soil.Aimed to select the best combination,the degradation rate of petroleum of each compound was assayed every 7 days.The results showed that the degradation rates of single inoculation with corn,teosinte and ryegrass were all higher than double inoculation.The three plants with two fungus expressed the best effect in oil contaminated soil of 1%.While the degradation rates of double inoculation with alfalfa reached the highest of 87% when the concentration was higher than 1.5%,and decreased with increasing of the oil concentration.The mixed-inoculation of alfalfa and two petroleum degraded fungus was more suitable for higher concentration of oil pollution.It had great values to promote for soil bioremediation.
The petroleum degradation of compound with four plants and two petroleum degraded fungus was discussed.Single or double of two fungus(Fungal sp.,Stachybotrys sp.) were respectively inoculated with four plants(corn,teosinte,ryegrass,alfalfa) into five oil concentrations(0.1%,0.5%,1%,1.5%,2.0%) soil.Aimed to select the best combination,the degradation rate of petroleum of each compound was assayed every 7 days.The results showed that the degradation rates of single inoculation with corn,teosinte and ryegrass were all higher than double inoculation.The three plants with two fungus expressed the best effect in oil contaminated soil of 1%.While the degradation rates of double inoculation with alfalfa reached the highest of 87% when the concentration was higher than 1.5%,and decreased with increasing of the oil concentration.The mixed-inoculation of alfalfa and two petroleum degraded fungus was more suitable for higher concentration of oil pollution.It had great values to promote for soil bioremediation.
引文
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[11]杨秀梅,陈保冬,朱永官,等.丛枝菌根真菌(Glomus in-traradices)对铜污染土壤上玉米生长的影响[J].生态学报,2008,29(3):1052-1056.
[12]赵淑清,田春杰,何兴元.固氮植物的菌根研究[J].应用生态学报,2000,11(2):306-310.
[13]吴良鸿,玛孜亚.浅谈紫花苜蓿栽培管理技术[J].新疆畜牧业,2010,11(6):46-48.
[14]汪洪钢,吴观以,李慧荃.VA菌根真菌与根瘤菌对离体绿豆根器官的侵染[J].微生物学通报,1990,17(4):193-195.
[15]Fitter A H,Garbaye J.Interaction between mycorrhizal fungi and other soil or ganisms[J].Plant and Soil,1994,159:123-132.
[16]罗启仕,张锡辉,王慧,等.生物修复中有机污染物的生物可利用性[J].生态环境,2004,13(1):85-89.
[2]李政,赵朝成,张云波,等.16种EPA-PAHs复合污染土壤的菌群修复[J].中国石油大学学报:自然科学版,2012,36(31):175-176.
[3]张天彬,杨国义,万洪富,等.东莞市土壤中多环芳烃的含量、代表物及其来源[J].土壤,2005,37(3):265-271.
[4]陈春云,岳珂,陈振明,等.微生物降解多环芳烃的研究进展[J],微生物学杂志,2007,27(6):100-101.
[5]杨发忠,颜阳,张泽志,等.多环芳烃研究进展[J].云南化工,2005,32(2):44-46.
[6]胡长庆,鲍玉婷,徐伟,等.丝状石油降解真菌的筛选及其降解特性研究[J].安徽农业科学,2010,38(4):1664-1666.
[7]胡长庆,徐伟,鲍玉婷,等.丝状真菌的混合菌群降解原油的条件与效果[J].安徽农业科学,2010,38(5):2562-2565.
[8]田林双.微生物降解多环芳烃研究[J].畜牧与饲料科学,2009,30(10):90-91.
[9]Vecchioli G I,Del Panno M T,Painceira M T.Use of se-lected autochthonous soil bacteria to enhance degradation of hydrocarbons in soil[J].Environmental Pollution,1990,67(3):249-258.
[10]林凤翱,冯志权,李洪,等.海洋丝状真菌降解原油研究—沙砾中油污去除室内模拟实验[J].海洋学报,1997,19(6):68-76.
[11]杨秀梅,陈保冬,朱永官,等.丛枝菌根真菌(Glomus in-traradices)对铜污染土壤上玉米生长的影响[J].生态学报,2008,29(3):1052-1056.
[12]赵淑清,田春杰,何兴元.固氮植物的菌根研究[J].应用生态学报,2000,11(2):306-310.
[13]吴良鸿,玛孜亚.浅谈紫花苜蓿栽培管理技术[J].新疆畜牧业,2010,11(6):46-48.
[14]汪洪钢,吴观以,李慧荃.VA菌根真菌与根瘤菌对离体绿豆根器官的侵染[J].微生物学通报,1990,17(4):193-195.
[15]Fitter A H,Garbaye J.Interaction between mycorrhizal fungi and other soil or ganisms[J].Plant and Soil,1994,159:123-132.
[16]罗启仕,张锡辉,王慧,等.生物修复中有机污染物的生物可利用性[J].生态环境,2004,13(1):85-89.