不同功率532nm激光诱导棕色挪威大鼠脉络膜新生血管模型的实验研究
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摘要
目的采用532 nm激光的不同功率诱导棕色挪威大鼠脉络膜新生血管模型,以探求成模率最高的激光功率。方法将45只挪威棕色大鼠随机分为3个实验组,每组15只,激光功率依次为400 mW、300 mW、200 mW,光斑直径100μm,曝光时间100 ms,每眼光凝10～12个点。对各实验组大鼠激光造模后的第1周、2周、3周、4周行眼底彩照、荧光素血管造影和光学相干断层扫描,对实验数据进行统计。结果眼底彩照可见激光诱导后的视网膜因玻璃膜的破坏呈现水肿状态;荧光血管造影显示功率200 mW的激光与300 mW、400 mW的激光诱导后第1～4周的渗漏率对比,差异有统计学意义(P<0.05);功率为300 mW和400 mW在第1、3周时,其渗漏率差异无统计学意义(P>0.05),第2、4周对比渗漏率有统计学意义(P<0.05);功率为200 mW的激光诱导第1、2周的渗漏率差异无统计学意义(P>0.05),而第1、3周和第2、3周的渗漏率差异有统计学意义(P<0.05),功率为300 mW和400 mW激光诱导第1、2、3周的渗漏率两两对比差异有统计学意义(P<0.05),3组功率在第3、4周的渗漏率对比,差异均无统计学意义(P>0.05)。光学相干断层扫描显示视网膜破坏后结果紊乱,然后诱发脉络膜新生血管形成,最终趋于稳定,部分视网膜结构萎缩变薄。结论 200 mW、300 mW、400 mW的532 nm激光均可以诱导挪威棕色大鼠形成脉络膜新生血管模型,其中300 mW的成模率最高,脉络膜新生血管于1周后开始形成,第3周达到高峰,第4周趋于稳定。
Objective To induce the choroidal neovascularization model of Brown Norwegian rats with different power of 532 nm laser,we would explore the highest fluorescein leakage rate by the best laser power. Method Forty-five Norwegian rats were randomly divided into three experimental groups: 15 rats in each group. The laser power were 400 mW, 300 mW, 200 mW, the spot diameter was 100 μm, the exposure time was 100 ms, and each eye was lasered 10～12 points. The experimental datas were collected from the 1 st～4 th week after lasering Brown Norway. The experimental datas were statistically analyzed by fundus fluorescein angiography and optical coherence tomography. Result The retina of the fundus showed that the retina had edema due to the destruction of the vitreous membrane after fluorescence induction. FFA showed that the the leakage rate of the laser power of 200 mW compared with 300 mW and 400 mW at the 1 st to 4 th was statistically significant(P<0.05). Compared 300 mW with 400 mW laser power,the leakage rate at the 1 st and 3 rd week was no statistically significant(P>0.05), and the leakage rate at the 2 nd and 4 th weeks was statistically significant(P<0.05). There was no significant difference in the leakage rate between the 1 st and 2 nd week of 200 mW laser power(P>0.05).But there was significant difference between the 1 st and 3 rd week of 200 mW laser power(P<0.05). It was significant difference between the 2 nd and 3 rd week of 200 mW laser power(P<0.05). The leakage rates induced by 300 mW and 400 mW laser power were statistically significant at the 1 st, 2 nd and 3 rd weeks(P<0.05). The leakage rates of the three groups were no statistical significance at the 3 rd and 4 th weeks(P>0.05). Optical coherence tomography showed retinal was disordered, then retinal begained to form choroidal neovascularization.The choroidal neovascularization was ultimately stabilized,and part of the retinal structure atrophy thinning. Conclusion 200 mW, 300 mW and 400 mW laser power of 532 nm laser can induce the formation of choroidal neovascularization model by Brown Norway. The formation rate of 300 mW laser power is the highest.The choroidal neovascularization begins to occur after the 1 st week, reaching the peak in the 3 rd week, and the choroidal neovascularization tends to be stable at the 4 rth week.
Objective To induce the choroidal neovascularization model of Brown Norwegian rats with different power of 532 nm laser,we would explore the highest fluorescein leakage rate by the best laser power. Method Forty-five Norwegian rats were randomly divided into three experimental groups: 15 rats in each group. The laser power were 400 mW, 300 mW, 200 mW, the spot diameter was 100 μm, the exposure time was 100 ms, and each eye was lasered 10～12 points. The experimental datas were collected from the 1 st～4 th week after lasering Brown Norway. The experimental datas were statistically analyzed by fundus fluorescein angiography and optical coherence tomography. Result The retina of the fundus showed that the retina had edema due to the destruction of the vitreous membrane after fluorescence induction. FFA showed that the the leakage rate of the laser power of 200 mW compared with 300 mW and 400 mW at the 1 st to 4 th was statistically significant(P<0.05). Compared 300 mW with 400 mW laser power,the leakage rate at the 1 st and 3 rd week was no statistically significant(P>0.05), and the leakage rate at the 2 nd and 4 th weeks was statistically significant(P<0.05). There was no significant difference in the leakage rate between the 1 st and 2 nd week of 200 mW laser power(P>0.05).But there was significant difference between the 1 st and 3 rd week of 200 mW laser power(P<0.05). It was significant difference between the 2 nd and 3 rd week of 200 mW laser power(P<0.05). The leakage rates induced by 300 mW and 400 mW laser power were statistically significant at the 1 st, 2 nd and 3 rd weeks(P<0.05). The leakage rates of the three groups were no statistical significance at the 3 rd and 4 th weeks(P>0.05). Optical coherence tomography showed retinal was disordered, then retinal begained to form choroidal neovascularization.The choroidal neovascularization was ultimately stabilized,and part of the retinal structure atrophy thinning. Conclusion 200 mW, 300 mW and 400 mW laser power of 532 nm laser can induce the formation of choroidal neovascularization model by Brown Norway. The formation rate of 300 mW laser power is the highest.The choroidal neovascularization begins to occur after the 1 st week, reaching the peak in the 3 rd week, and the choroidal neovascularization tends to be stable at the 4 rth week.
引文
[1] Campa C,Costagliola C, Incorvaia C, et al. Inflammatory Mediators and Angiogenic Factors in Choroidal Neovascularization: Pathogenetic Interactions and Therapeutic Implications, Mediators of Inflammation, vol. 2010, Article ID 546826, 14 pages, 2010. doi:10.1155/2010/546826.
[2] 唐坤. MIL60治疗激光诱导BN大鼠脉络膜新生血管的实验研究[D].北京:中国人民解放军医学院,2014.
[3] 张舒阳,刘颖,陈珂,等. Nd:YAG激光诱导棕色挪威大鼠脉络膜新生血管模型的建立与评价[J]. 眼科新进展,2016,04:318-322.
[4] 任秉仪,刘安,郑永征,等. 532nm激光诱导棕色挪威大鼠脉络膜新生血管模型的研究与评价[J]. 上海医学,2013,04:358-360+269.
[5] Li Q, Timmers A M, Hunter K, et al.Noninvasive imaging by optical coherence tomography to monitor retinal degenera-tion in the mouse[J].Invest Ophthalmol Vis Sci, 2001, 42(1):2981-2989.
[6] Fukuchi T, Takahashi K, Shou K, et al.Optical coherence tomography (OCT) findings in normal retina and laser-in-duced choroidal neovascularization in rats[J].Graefes ArchClin Exp Ophthalmol, 2001, 239(1):41-46.
[7] Kim S G, Lee S C, Seong Y S, et al.Choroidal neovascular-ization characteristics and its size in optical coherence to-mography[ J].Yonsei Med J, 2003, 44(5):821-827.
[8] Olson J L, Courtney R J,Mandava N. Intravitreal infliximab and choroidal neovascularization in an animal model[J], Arch Ophthalmol, 2007,125(9): 1221-1224.
[9] 赵世红,何守志.気激光诱导的大鼠脉络膜新生血管模型研宄[J].中华眼科杂志,2003,39(5):298-302.
[10] 史雪辉,何守志,赵世红等.色素上皮衍生因子在大鼠脉络膜新生血管中的表达及意义[J].中华眼科杂志,2004, 40(6):404-408.
[11] Fu Y,Ponce M L, Thill M,et al. Angiogenesis inhibition and choroidal neovascularization suppression by sustained delivery of an integrin antagonist EMD478761[J]. Invest Ophthalmol Vis Sci,2007,48(11): 5184-5190.
[12] 李学晶,唐由之,王慧娟,等. 脉络膜新生血管动物模型的建立与评估[J]. 国际眼科杂志,2009,2:246-249.
[13] 王鑫. 实验性脉络膜新生血管中Rap1及相关因子表达的研究[D].石家庄:河北医科大学,2015.
[2] 唐坤. MIL60治疗激光诱导BN大鼠脉络膜新生血管的实验研究[D].北京:中国人民解放军医学院,2014.
[3] 张舒阳,刘颖,陈珂,等. Nd:YAG激光诱导棕色挪威大鼠脉络膜新生血管模型的建立与评价[J]. 眼科新进展,2016,04:318-322.
[4] 任秉仪,刘安,郑永征,等. 532nm激光诱导棕色挪威大鼠脉络膜新生血管模型的研究与评价[J]. 上海医学,2013,04:358-360+269.
[5] Li Q, Timmers A M, Hunter K, et al.Noninvasive imaging by optical coherence tomography to monitor retinal degenera-tion in the mouse[J].Invest Ophthalmol Vis Sci, 2001, 42(1):2981-2989.
[6] Fukuchi T, Takahashi K, Shou K, et al.Optical coherence tomography (OCT) findings in normal retina and laser-in-duced choroidal neovascularization in rats[J].Graefes ArchClin Exp Ophthalmol, 2001, 239(1):41-46.
[7] Kim S G, Lee S C, Seong Y S, et al.Choroidal neovascular-ization characteristics and its size in optical coherence to-mography[ J].Yonsei Med J, 2003, 44(5):821-827.
[8] Olson J L, Courtney R J,Mandava N. Intravitreal infliximab and choroidal neovascularization in an animal model[J], Arch Ophthalmol, 2007,125(9): 1221-1224.
[9] 赵世红,何守志.気激光诱导的大鼠脉络膜新生血管模型研宄[J].中华眼科杂志,2003,39(5):298-302.
[10] 史雪辉,何守志,赵世红等.色素上皮衍生因子在大鼠脉络膜新生血管中的表达及意义[J].中华眼科杂志,2004, 40(6):404-408.
[11] Fu Y,Ponce M L, Thill M,et al. Angiogenesis inhibition and choroidal neovascularization suppression by sustained delivery of an integrin antagonist EMD478761[J]. Invest Ophthalmol Vis Sci,2007,48(11): 5184-5190.
[12] 李学晶,唐由之,王慧娟,等. 脉络膜新生血管动物模型的建立与评估[J]. 国际眼科杂志,2009,2:246-249.
[13] 王鑫. 实验性脉络膜新生血管中Rap1及相关因子表达的研究[D].石家庄:河北医科大学,2015.