乳制品中氰化物的快速检测
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摘要
基于是食品中氰化物衍生化及顶空气相色谱技术,本实验尝试建立了一种适用于乳制品样品中氰化物含量快速检测方法,同时,对检测过程中衍生剂用量、衍生p H值、气相顶空平衡时间和平衡温度等条件进行优化。利用该方法对20种不同品牌乳制品中的氰化物含量进行分析检测,实验结果表明,该方法具有良好的准确度和灵敏度,氰离子在0.001 mg/L～0.1 mg/L范围内线性关系良好,最低检出限为0.00056 mg/L,相对标准偏差值低于10%,回收率在86.9%～91.8%,稳定性好,抗干扰能力强,在实际生产中具有很好的应用价值。
Based on cyanide derivatization and headspace gas chromatography in food,a rapid method for detection of cyanide content in dairy products was established in this experiment.At the same time,the amount of derivatized agent,derived pH value,gas phase headspace equilibrium time and equilibrium temperature were optimized.The method is used to analyze the cyanide content in 20 different brands of dairy products.The experimental results show that the method has good accuracy and sensitivity.The linear relationship of cyanide ions in the range of 0.001 mg/L to 0.1 mg/L is good,the minimum detection limit is 0.00056 mg/L,the standard deviation value is lower than10 %,and the recovery rate is 86.9 %～91.8 % has good stability and strong anti-interference ability.It has very good application value in actual production.
Based on cyanide derivatization and headspace gas chromatography in food,a rapid method for detection of cyanide content in dairy products was established in this experiment.At the same time,the amount of derivatized agent,derived pH value,gas phase headspace equilibrium time and equilibrium temperature were optimized.The method is used to analyze the cyanide content in 20 different brands of dairy products.The experimental results show that the method has good accuracy and sensitivity.The linear relationship of cyanide ions in the range of 0.001 mg/L to 0.1 mg/L is good,the minimum detection limit is 0.00056 mg/L,the standard deviation value is lower than10 %,and the recovery rate is 86.9 %～91.8 % has good stability and strong anti-interference ability.It has very good application value in actual production.
引文
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[16]杜利君,刘晓林,高媛惠,等.顶空气相色谱法快速测定酒中氰化物含量[J].食品研究与开发,2017,38(11):29.
[17]刘芯韵,张坤,易萧.离子色谱法测定蒸馏酒中氰化物含量的方法性能分析[J].食品与发酵科技,2017,53(2):94.
[18]邢丽红,孙伟红,苗钧魁,等.LC-MS/MS内标法测定乳制品中的三聚氰酸[J].食品科学,2011,32(4):143-147.
[19]孟梁,张强.金属配位剂衍生-高效液相色谱法测定粮食及白酒中的游离氰化物[J].分析科学学报,2009,25(5):587-892.
[2]陈历水,丁庆波.我国乳制品发展现状及趋势[J].乳业科学与技术,2012,6(35):37-42.
[3]魏传立,王福林.我国乳品工业的现状与发展研究[J].农机化研究,2010,18(1):24-28.
[4]刘程,李浩林,刘箐,等..乳制品中有害成分检测技术研究进展[J].食品科学,2013,21(34):425-431.
[5]付宝森.影响中国乳制品安全的因素分析及对策研究[J].沈阳大学学报,2011,12(6):10-30.
[6]JEFFREY J ROSENTRETER,YEGOR G TIMOFEYENKO,MOISES MORENO.Safe routine cyanide detection methodology for aqueous solutions of varied pH[J].Microchemical 119(2015)17-21.
[7]SHARIFAH MAZRAH SAYED MOHAMED ZAIN,RAFIZASHAHARUDIN,MUHAMMAD AMIR KAMALUDDIN,et al.Determination of hydrogen cyanide in residential ambient air usingSPME coupled with GC-MS[J].Atmospheric Pollution Research2017,38(17)1-8.
[8]刘震.鲜乳制品中三聚氰胺的高效液相色谱法检测[J].科技创新与应用,2012,56(1):27-32.
[9]吴建平,杨建青.中国奶业原料奶生产组织模式及效率研究[D].北京:中国农业科学院,2009.
[10]XIAO ZHANG YU,YUJUAN LIN,CHUNJIAO LU,ER AL.Microarray-based expression analysis of phytohormone-related genes in rice seedlings during cyanide metabolism[J].Environmental Science and Pollution Research.2018,16(23):13-18.
[11]氰化物的危害和销毁技术研究进展[J].环境科技,2009,1(22):79-81.
[12]XU YIGANG,CUI LICHUN,TIAN CHANGYONG,et al.Amultiplex polymerase chain reaction coupled with high performance liquid chromatography assay for simultaneous detection of six foodborne pathogens[J].Food Control,2012,25(2):778-783.
[13]LI XIANGMEI,LUO PENGJIE,TANG SHUSHENG,et al.Development of an immunochromatographic strip test for rapid detection of melamine in raw milk,milk products and animal feed[J].Journal of Agricultural and Food Chemistry,2011,59(11):6064-6070.
[14]ZABLON K N.Rapid and sensitive detection of human african trypanosomiasis by loop-mediated isothermal amplification combined with a lateral-flow dipstick[J].Diagnostic Microbiology&Infectious Disease,2011,69(2):205-209.
[15]朱晓玲.水中微量氰化物的异烟酸吡唑啉酮分光光度测定法进展[J].北方环境,2011,23(4):140.
[16]杜利君,刘晓林,高媛惠,等.顶空气相色谱法快速测定酒中氰化物含量[J].食品研究与开发,2017,38(11):29.
[17]刘芯韵,张坤,易萧.离子色谱法测定蒸馏酒中氰化物含量的方法性能分析[J].食品与发酵科技,2017,53(2):94.
[18]邢丽红,孙伟红,苗钧魁,等.LC-MS/MS内标法测定乳制品中的三聚氰酸[J].食品科学,2011,32(4):143-147.
[19]孟梁,张强.金属配位剂衍生-高效液相色谱法测定粮食及白酒中的游离氰化物[J].分析科学学报,2009,25(5):587-892.